Assessment of serological response of young and adult sheep to conjunctival vaccination with Rev-1 vaccine by fluorescence polarization assay (FPA) and other serological tests for B. melitensis

Stournara, Athanasia; Minas, Anastasios; Bourtzi-Chatzopoulou, E.; Stack, J.; Koptopoulos, G.; Petridou, Evanthia; Sarris, K.

doi:10.1016/j.vetmic.2006.08.004

Cited by 17 publications

(20 citation statements)

References 14 publications

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“…The percentage of positive animals detected by iELISA and mRBT decreased rapidly from 6 weeks post-vaccination to the end of study. Similar performance of iELISA and mRBT in our study is in agreement with results of the study carried out by Stournara et al 9 However, in the latter study 72.6% and 84% of animals remained positive in iELISA and mRBT, respectively 91 days after immunization, but in our study only 16 ence may be due to age and physiologic status of animals when vaccinated or breed variation in antibody responses. 7,18 Evaluation of serological responses during the study period using SAT revealed antibody titers fell from the peak reached at week two toward the end of study.…”

Section: Discussionsupporting

confidence: 93%

“…A similar result has been observed by Stournara et al 9 which was attributed to the higher affinity of the conjugate used in the assay to immunoglobulin G (IgG). It has also been reported that more than 70 percent of non-vaccinated ewes were detected as negative by iELISA 14 days after challenge with the virulent strain during pregnancy.…”

Section: Discussionsupporting

confidence: 83%

“…6,8,10,16 In our experiment, all animals were detected as positive using mRBT two weeks after immunization. Stournara et al 9 also reported a hundred percent positive results in mRBT of non-pregnant ewes 21 days post-vaccination. In another study by Zundel et al, 16 all ewes vaccinated at mid-pregnancy using the same dose as ours were positive in RBT 2 weeks following vaccination.…”

Section: Discussionmentioning

confidence: 92%

“…It has also been reported that more than 70 percent of non-vaccinated ewes were detected as negative by iELISA 14 days after challenge with the virulent strain during pregnancy. 17 Because the ewes used in our study were from a brucellosis-free flock without previous exposure to the pathogen, and according to the explanation provided by Stournara et al, 9 negative iELISA results of these naive ewes two weeks after vaccine inoculation suggest antibody responses may be mainly of IgM class at this time. The percentage of positive animals detected by iELISA and mRBT decreased rapidly from 6 weeks post-vaccination to the end of study.…”

Section: Discussionmentioning

confidence: 94%

“…[6][7][8] One problem with vaccination of adult animals is antibody responses induced by the vaccine which may last for a long time and cause sero-positivity of vaccinated animals in routine serological tests interfering with detection of infected ones. 4,9,10 This makes simultaneous implementation of vaccination and testand-slaughter impossible since vaccinated animals are falsely diagnosed as infected. 4 Moreover, vaccine-induced abortion and vaccine strain excretion in milk and vaginal discharges may occur.…”

Section: Introductionmentioning

confidence: 99%

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Innocuousness of conjunctival vaccination with Brucella melitensis strain Rev.1 in pregnant Iranian fat-tailed ewes

et al. 2015

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Brucella melitensis strain Rev.1 is the most effective vaccine against brucellosis in sheep and goats. In Iran, mass vaccination is carried out all over the country in which adult animals are immunized by subcutaneous injection of reduced doses of the vaccine. However, due to antibody responses elicited by vaccination, concomitant implementation of test-andslaughter is impossible. To overcome the problem, vaccination through conjunctival route is recommended. In this study, serological responses of six pregnant Iranian fat-tailed ewes to conjunctival vaccination with standard doses of the vaccine were evaluated using modified Rose Bengal test, serum agglutination test and indirect ELISA. Besides, vaccine strain excretion in milk and vaginal discharges was also examined by microbiological culture of milk and vaginal swab samples taken one day post-parturition. Animals were vaccinated during the second half of gestation. As the results, antibody titers of five (83.3%) ewes decreased to the levels not detectable by the tests within three months after vaccination. No vaccine-induced abortions occurred and vaccinated ewes delivered healthy lambs 50.33±15.56 (mean ± standard deviation) days post-vaccination. Vaccine strain was not isolated from milk and vaginal swab samples. Generally, our study shows full doses of B. melitensis strain Rev.1 can be used conjunctively to vaccinate pregnant Iranian sheep during late pregnancy without abortifacient effects, prolonged antibody responses and vaccine strain excretion in milk and vaginal discharges. Nevertheless, further studies are required to determine safety and immunogenicity of the vaccine in field conditions.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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Innocuousness of conjunctival vaccination with Brucella melitensis strain Rev.1 in pregnant Iranian fat-tailed ewes

et al. 2015

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The effects of the conjunctival Brucella vaccine on some biochemical parameters in sheep

Çiftçi

Yiğit

Çiftci

2018

Trop Anim Health Prod

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The genus Brucella causes significant economic losses due to infertility, abortion, stillbirth or weak calves, and neonatal mortality in livestock. Brucellosis is still a zoonosis of public health importance worldwide. In the past, vaccination was administered subcutaneously and nowadays, the conjunctival vaccine is administered. There is no definite information about the changes of the biochemical parameters and antibody response after conjunctival vaccination. In this study, the investigation of the changes in the levels of some biochemical parameters due to the conjunctival vaccination for brucellosis was aimed. Thirty sheep were used as an animal material. The vaccine was done single dose against Brucella melitensis and the blood was drawled from Vena jugularis during 4 months. Antibody levels were determined by serum tube agglutination test. Alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), glucose, total protein, and albumin levels were measured using commercial autoanalyzer in sera. The antibody titers (p < 0.001) increased significantly at first month compared to the pre-vaccination, but at the second month began to fall. There was no statistically significant changes in glucose, AST, ALT activity after vaccination (p > 0.005). The significant amount of total protein and ALP decreased after vaccination (p < 0.005). LDH levels and total protein levels were significantly increased (p < 0.005). In conclusion, conjunctival vaccine was considered to be used as a safe to protect the sheep from brucellosis and the results of the study may be used to improve the efficiency of brucellosis eradication programs within livestock management.

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Molecular detection of Brucella spp. in ruminant herds in Greece

Katsiolis

Papanikolaou

Stournara³

et al. 2022

Trop Anim Health Prod

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Assessment of serological response of young and adult sheep to conjunctival vaccination with Rev-1 vaccine by fluorescence polarization assay (FPA) and other serological tests for B. melitensis

Cited by 17 publications

References 14 publications

Innocuousness of conjunctival vaccination with Brucella melitensis strain Rev.1 in pregnant Iranian fat-tailed ewes

Innocuousness of conjunctival vaccination with Brucella melitensis strain Rev.1 in pregnant Iranian fat-tailed ewes

The effects of the conjunctival Brucella vaccine on some biochemical parameters in sheep

Molecular detection of Brucella spp. in ruminant herds in Greece

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