Assessment of seminal mast cells in infertile men with varicocele after surgical repair

Mostafa, Rashad M.; Abol-Magd, R.; Younis, Soha; Dessouki, Omar; Azab, M. M.; Mostafa, Taymour

doi:10.1111/and.12625

Cited by 8 publications

(5 citation statements)

References 33 publications

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“…Lately, Mostafa et al . () reported that in infertile patients with Vx ( n = 45), there was an increased number of MCs in their semen that was significantly decreased after Vx repair.…”

Section: Discussionmentioning

confidence: 99%

Effect of varicocelectomy and/or mast cells stabilizer on sperm DNA fragmentation in infertile patients with varicocele

et al. 2017

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This study aimed to assess the effect of varicocelectomy and/or mast cells (MCs) stabilizer on sperm DNA fragmentation in infertile men with varicocele (Vx). Overall, 120 infertile patients were randomized to three equal treatment arms; patients that underwent varicocelectomy, patients on 1 mg ketotifen twice daily for three months, and patients that underwent varicocelectomy followed with 1 mg ketotifen twice daily for three months. These patients were subjected to history taking, clinical examination, semen analysis, and estimation of sperm DNA fragmentation index (DFI). After 3 months, all investigated groups showed significant improvement regarding the mean total sperm count, sperm concentration, total sperm motility, and sperm normal forms percentage compared with the pre-treatment data. As well, the mean sperm DFI was significantly improved compared with the pre-treatment data; in men that underwent varicocelectomy (34.6% vs. 28.3%), in men on MC stabilizer only (33.4% vs. 27.8%), and in men that underwent varicocelectomy followed by MC stabilizer (34.3% vs. 25.1%). Sperm DFI improvement percentages showed the highest improvement in men that underwent varicocelectomy followed with MC stabilizer compared with the other two groups (26.8% vs. 18.2%, 16.8%). Sperm DFI improvement percentages showed significant increases in the infertile patients with Vx grade III compared to Vx grade II in all investigated groups. It is concluded that in infertile men associated with Vx and high sperm DFI, surgical repair followed with MCs stabilizer significantly improve sperm DFI compared with either surgical repair or MCs stabilizer alone.

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“…Lately, Mostafa et al . () reported that in infertile patients with Vx ( n = 45), there was an increased number of MCs in their semen that was significantly decreased after Vx repair.…”

Section: Discussionmentioning

confidence: 99%

Effect of varicocelectomy and/or mast cells stabilizer on sperm DNA fragmentation in infertile patients with varicocele

et al. 2017

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“…A higher number of mast cells in the interstitium and lamina propria has been associated with different causes of infertility, including varicocele 18 . In a 2017 study by Mostafa et al, a significant reduction in the number of seminal mast cells was found after varicocelectomy 19 . As mast cells and their products play a role in the pathogenesis of asthenozoospermia, it suggests a possible target for the medical treatment of infertile men.…”

Section: Discussionmentioning

confidence: 99%

“… 18 In a 2017 study by Mostafa et al, a significant reduction in the number of seminal mast cells was found after varicocelectomy. 19 As mast cells and their products play a role in the pathogenesis of asthenozoospermia, it suggests a possible target for the medical treatment of infertile men. Therefore, drugs that could block mast cell mediator release may have a beneficial effect on sperm development.…”

Section: Discussionmentioning

confidence: 99%

Semen's parameters after varicocelectomy surgery with mast cell stabilizers treatment in infertile varicocele patients: Randomized clinical trial study

Akhavan Rezayat,

Soleimanian,

Kamandi

et al. 2023

Health Science Reports

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Background and Aims: Varicocele is one of the most common causes of male infertility in which testicular function is progressively damaged. This study aims to investigate the effect of varicocelectomy and mast cell stabilizers on reducing sperm DNA fragmentation in infertile patients with varicocele.Methods: In this randomized clinical trial, after obtaining ethical approval and informed consent, infertile patients with varicocele were randomly assigned to three groups: varicocelectomy, 1 mg ketotifen, and varicocelectomy plus with 1 mg ketotifen for 3-month follow-up. Semen analysis and estimation of DNA fragmentation index (DFI) were obtained from all people in the studied time periods.Results: In this study, 420 infertile patients with varicocele were included with an average age of 31.6 years. The results showed that sperm morphology, sperm motility, sperm count, and sperm DFI had significant differences after the intervention (p < 0.05). Also, the results of semen analysis for each group showed that there was a significant improvement in sperm morphology, motility, count, and DFI index of infertile people with varicocele after the intervention (p < 0.05). But the difference was statistically higher in the varicocelectomy plus with 1 mg ketotifen group than in the other groups (p < 0.01). Conclusion:The evidence and results of this study showed that the use of varicocelectomy plus ketotifen in infertile people with varicocele was more effective in improving Semen parameters than the use of mast cell stabilizers or varicocelectomy alone.

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“…Cincik et al reported the negative effects of seminal MCs on sperm concentration, motility, and morphology after studying 400 semen samples and staining MCs by toluidine blue‐pyronine. In varicocele, seminal MCs increasing correlates with the grade laterality of the disease but post‐varicocelectomy decreases their number in a negative correlation with sperm parameters . Tryptase after being released from MCs in the seminal fluid may reduce sperm parameters mainly motility in a dose‐ and time‐dependent manner through acting on PAR‐2 via ERK1/2 pathway …”

Section: Male Reproduction System Residing Mast Cellsmentioning

confidence: 99%

Significance of mast cells in spermatogenesis, implantation, pregnancy, and abortion: Cross talk and molecular mechanisms

Komi

Shafaghat

Haidl

2020

American J Rep Immunol

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Both subsets of MCs including MCTC (tryptase‐positive, chymase‐positive) and MCT (tryptase‐positive, chymase‐negative) are present in the testis and epididymis. Increased number of MCs, higher levels of MC‐released tryptase in testis and seminal plasma of males with fertility problems, and promoting sperm motility in individuals with oligozoospermia after using MC blockers provide evidence that MCs may play a role in male infertility/subfertility disturbances. MC‐released tryptase and histamine contribute to the fibrosis and may disrupt spermatogenesis. MCs not only influence the process of spermatogenesis but also have effects on the function of other testis‐residing cells. MC‐derived histamine may influence the steroidogenesis of Leydig cells by acting through H1R and H2R receptors. Additionally, the interaction between MC‐released ATP and P2X receptors expressed on the peritubular cells may induce the production of the pro‐inflammatory mediators by peritubular cells. Further investigations showed that MCs may be involved in the pathology of female infertility during implantation, pregnancy, and abortion. In the uterus, MCT subtype is abundant in myometrium and adjacent basal layer while MCTC subtype is distributed in all layers. MCs in response to hormones mainly estradiol and progesterone become activated and release a wide range of mediators including histamine, VEGF, proteases, and metalloproteinases (MMPs) that have a role in different stages of pregnancy. An increasing influx of MCs to the cervix during the pregnancy occurs that helps to the physiologic cervical ripening. While MMPs degrade the extracellular matrix (ECM), VEGF modulates neovascularization and histamine influences the embryo implantation. MC‐derived histamine may have a positive effect during implantation due to its participation in tissue remodeling. MC proteases including tryptase and chymase activate the precursors of MMP2 and MMP9 to mediate ECM degradation during the physiologic menstrual cycle. There is a line of evidence that MCs have a role in abortion by releasing TNF‐α.

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Assessment of seminal mast cells in infertile men with varicocele after surgical repair

Cited by 8 publications

References 33 publications

Effect of varicocelectomy and/or mast cells stabilizer on sperm DNA fragmentation in infertile patients with varicocele

Effect of varicocelectomy and/or mast cells stabilizer on sperm DNA fragmentation in infertile patients with varicocele

Semen's parameters after varicocelectomy surgery with mast cell stabilizers treatment in infertile varicocele patients: Randomized clinical trial study

Significance of mast cells in spermatogenesis, implantation, pregnancy, and abortion: Cross talk and molecular mechanisms

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