Assessment of semen quality and anti‐oxidative enzyme activity between bovine sex‐sorted and non‐sex‐sorted frozen–thawed semen

Guo, Yanmin; Khan, Rajwali; Faisal, Muhammad; Zhang, Fan; Zhao, Fuwei; Ge, Shihao; Chu, Hanping; Zhongfeng, Wei; Zhao, X.-L.; Ayari-Akkari, Amel; Yassin, Habab M.

doi:10.1111/rda.14333

Cited by 4 publications

(6 citation statements)

References 20 publications

(36 reference statements)

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“…For instance, the commercially employed method (flow cytometry) for sorting semen kills the sperm cells during selection (27). Other studies have reported a noticeable decrease in sperm quality following sexing semen (45,46).…”

Section: Discussionmentioning

confidence: 93%

“…Despite the low conception rate following the use of sexed semen in goats, sheep, and cattle, the method is accurate (39,40,44). This finding, therefore, necessitates more innovation studies to come up with a sexing technology that will protect sperm cell injuries during sorting, ehich was supported by the evidence from Silva et al's (45) study in frozen-thawed goats' sperm cells and Guo et al's (46) study in frozen-thawed cattle's sperm cells. However, the type of insemination used in goats, sheep, and cattle also affects the conception rate following the use of sexed semen (12,35,37,41,59).…”

Section: Discussionmentioning

confidence: 98%

“…Cryopreservation also alters the distribution of proteins, such as glutathione peroxidase, glutathione reductase, and superoxide dismutase, that are necessary to scavenge ROS ( 60 ). A recent study by Guo et al ( 46 ) observed a massive reduction of natural antioxidants in sexed semen ( Table 6 ). This finding implies that poor conception rate and embryo development following the use of sexed semen can be caused by lower antioxidant defense against ROS.…”

Section: Limitations Associated With Sexed Semenmentioning

confidence: 90%

“…Flow cytometry selects male and female sperm cells and discards dead and abnormal sperm cells during the process ( 34 ). This process leads to the hypothesis that the sex-sorted semen has lower sperm quality in comparison to the non-sex-sorted semen ( 46 ), which is because the speed, pressure, laser light, electrical charging, processes involved in sorting semen deviation, and changes in the medium jointly lead to defects that can damage sperm cells ( 31 , 47 ).…”

Section: Prospects Of Sex-sorted Semen In Conservation Programsmentioning

confidence: 99%

“…According to Pintus et al ( 81 ), oxidative stress occurs in the sperm cell when the amount of reactive oxygen species overcomes the level of antioxidants. Guo et al ( 46 ) observed a relatively low antioxidant enzyme (GSH; SOD; CAT; and GSH-Px) and the rate of fertilization activities in the sex-sorted semen. In this study, it was concluded that these less enzymatic activities observed in the sex-sorted semen were caused by the complex processes of sorting semen.…”

Section: Different Ways To Improve Sex-sorted Semen Quality and Ferti...mentioning

confidence: 99%

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A systematic review on the prospects of X- and Y-sexed semen in ruminant livestock: implications for conservation, a South African perspective

Ngcobo,

Nedambale,

Sithole

et al. 2024

Front. Vet. Sci.

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South Africa is home to numerous indigenous and locally developed sheep (Nguni Pedi, Zulu, and Namaqua Afrikaner, Afrino, Africander, Bezuidenhout Africander, Damara, Dorper, Döhne Merino, Meat Master, South African Merino, South African Mutton Merino, Van Rooy, and Dorper), goat (SA veld, Tankwa, Imbuzi, Bantu, Boer, and Savanna) and cattle (Afrigus, Afrikaner, Bolowana, Bonsmara, Bovelder, Drakensberger, South African Angus, South African Dairy Swiss, South African Friesland, South African Red, and Veld Master) animals. These breeds require less veterinary service, feed, management efforts, provide income to rural and or poor owners. However, most of them are under extinction risks and some with unknown status hence, require immediate conservation intervention. To allow faster genetic progress on the endangered animals, it is important to generate productive animals while reducing wastages and this can be achieved through sex-sorted semen. Therefore, this systematic review is aimed to evaluate the prospects of X and Y-sexed semen in ruminant livestock and some solutions that can be used to address poor sex-sorted semen and its fertility. This review was incorporated through gathering and assessing relevant articles and through the data from the DAD-IS database. The keywords that were used to search articles online were pre-gender selection, indigenous ecotypes, fertility, flow cytometry, artificial insemination, conservation, and improving sexed semen. Following a careful review of all articles, PRISMA guidelines were used to find the articles that are suitable to address the aim of this review. Sex-sorted semen is a recently introduced technology gaining more attention from researchers particularly, in the conservation programs. Preselection of semen based on the sex chromosomes (X- and or Y-bearing chromosomes) is of paramount importance to obtain desired sex of the offspring and avoid animal wastage as much as possible. However, diverse factors can affect quality of semen of different animal species especially after sex-sorting. Flow cytometry is a common method used to select male and female sperm cells and discard dead and abnormal sperm cells during the process. Thus, sperm sexing is a good advanced reproductive technology (ART) however, it is associated with the production of oxidative stress (OS) and DNA fragmentation (SDF). These findings, therefore, necessitates more innovation studies to come up with a sexing technology that will protect sperm cell injuries during sorting in frozen-thawed.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 98%

Section: Limitations Associated With Sexed Semenmentioning

confidence: 90%

Section: Prospects Of Sex-sorted Semen In Conservation Programsmentioning

confidence: 99%

Section: Different Ways To Improve Sex-sorted Semen Quality and Ferti...mentioning

confidence: 99%

See 3 more Smart Citations

A systematic review on the prospects of X- and Y-sexed semen in ruminant livestock: implications for conservation, a South African perspective

Ngcobo,

Nedambale,

Sithole

et al. 2024

Front. Vet. Sci.

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Development of a Low-Cost Livestock Sorting Information Management System Leveraging Deep Learning, AI, and IoT Technologies

Shwetabhand,

Ambhaikar

2024

BIO Web Conf.

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The implementation of effective livestock management methods is crucial to optimize agricultural operations. However, conventional livestock sorting and data management approaches encounter several obstacles regarding precision, labor requirements, and financial implications. The process exhibits inefficiency, increased labor costs, and an elevated risk of zoonotic infections. Housing livestock in extensive groups might intensify the transmission of diseases and complicate the surveillance and management of diseased animals. This study attempted to develop a Low-Cost Livestock Sorting Information Management System (LC-LSIMS) using a dataset enriched with crucial metrics and curated images collected over 24 months with the Internet of Things (IoT) and Artificial Intelligence (AI). The design of edge-cloud computing facilitates the redistribution of computational resources, leading to enhanced computational speed. The LC-LSIMS would have a predictive module to assist agricultural practitioners in safeguarding their crops during flood occurrences. This module will empower farmers to proactively anticipate natural phenomena, including floods, during intense rainfall. LC-LSIMS presents a multi-level design plan that facilitates attaining the specified goals. The findings obtained from the execution of the implemented system demonstrate a sorting accuracy of 91.47%, computational speed of 27.42 frames per second (fps), labor cost reduction of 50.84%, production efficiency improvement of 29.59%, and an average reduction in data input errors of 37.59%.

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The Difference in Subpopulation Structures in Sex-Sorted and Non-Sorted Semen by Discriminant Analysis of Bull Sperm Motility Data Collected by a Computer-Assisted Sperm Analysis System

et al. 2023

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We examined the motility characteristics of sperm from sex-sorted bull semen as a model for low-quality (expected low conception rate) sperm compared to that of sperm from non-sorted semen (expected average conception rate) using cluster analysis followed by discriminant analysis. The results indicated that sex-sorted semen contained sperm with hyperactivation-like motility as the main subpopulation immediately after thawing, and this subpopulation decreased after 2 h of incubation. The main subpopulation in the non-sorted semen had progressive motility that was maintained during incubation. A conventional comparison of the mean values of kinematic parameters could not distinguish the samples. In conclusion, discriminant analysis using data from a cluster analysis of motile sperm may accurately describe differences in the structures of sperm motility subpopulations between low and normal fertility semen.

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Assessment of semen quality and anti‐oxidative enzyme activity between bovine sex‐sorted and non‐sex‐sorted frozen–thawed semen

Cited by 4 publications

References 20 publications

A systematic review on the prospects of X- and Y-sexed semen in ruminant livestock: implications for conservation, a South African perspective

A systematic review on the prospects of X- and Y-sexed semen in ruminant livestock: implications for conservation, a South African perspective

Development of a Low-Cost Livestock Sorting Information Management System Leveraging Deep Learning, AI, and IoT Technologies

The Difference in Subpopulation Structures in Sex-Sorted and Non-Sorted Semen by Discriminant Analysis of Bull Sperm Motility Data Collected by a Computer-Assisted Sperm Analysis System

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