“…This result is true and reasonable as the adjacent area of Site 6 and 7 is A6 that discharged untreated sewage directly to those sites [45, 46], that have the worst water quality with very high BOD, nutrients (NO3-N and PO4-P), NH3-N, EC, and TDS with very low dissolved oxygen. Untreated sewage has high BOD, nutrients (NO3-N and PO4-P), NH3-N, EC, and TDS with very low or almost zero dissolved oxygen [47]. However, the combination (A2, A3, & A6) in Site 4 to 5 and the combination (A2, A3, A5, & A6) in Site 5 to 6 also have urban settlement (A6), which contributed insignificantly because urban settlement covered only a smaller land cover, is far from the river, and sewage outfall is absent in Site 4 and lower in Site 5.…”