Assessment of SARS-CoV-2 neutralizing antibody titers in breastmilk from convalescent and vaccinated mothers

Bäuerl, Christine; Zulaica, Joao; Rusu, Luciana; Moreno, Alicia Rodríguez; Pérez-Cano, Francisco J.; Lerín, Carles; Mena‐Tudela, Desirée; Aguilar-Camprubí, Laia; Parra-Llorca, Anna; Martínez‐Costa, Cecilia; Geller, Ron; Collado, María Carmen

doi:10.1016/j.isci.2023.106802

Cited by 2 publications

(1 citation statement)

References 38 publications

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“… 32 , 33 , 34 In addition, we did see a significant increase in RBD-blocking activity after infection, suggesting again that milk antibodies have the capacity to neutralize the SARS-CoV-2 virus. 14 , 21 , 35 , 36 …”

Section: Discussionmentioning

confidence: 99%

Milk antibody response after 3rd COVID-19 vaccine and SARS-CoV-2 infection and implications for infant protection

Golan,

Ilala,

et al. 2023

iScience

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Section: Discussionmentioning

confidence: 99%

Milk antibody response after 3rd COVID-19 vaccine and SARS-CoV-2 infection and implications for infant protection

Golan,

Ilala,

et al. 2023

iScience

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SARS-CoV-2 Antibody Profiles in Maternal Serum and Breast Milk Following mRNA COVID-19 Vaccination: A Longitudinal Prospective Observational Cohort Study

Hsiao,

DiMaggio,

Perez

et al. 2023

Vaccines

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COVID-19 vaccination during pregnancy protects infants against symptomatic COVID-19. Vaccination of lactating mothers may offer additional protection, but our understanding of immune responses in breast milk is limited. We, therefore, performed a single-center prospective cohort study of lactating mothers who received a COVID-19 mRNA primary vaccine series to evaluate the durability, breadth, and neutralizing capacity of the antibody responses in breast milk. Spike IgG- and IgA-binding antibodies of ancestral SARS-CoV-2 in serum and breast milk were quantified over 9 months using Meso Scale Discovery (MSD) V-PLEX assays, and ancestral titers were compared to four variants of concern (Alpha, Beta, Delta, Gamma) at a single time point. Neutralizing antibodies against ancestral SARS-CoV-2 and Omicron BA.4/5 were compared before and after vaccination using a pseudovirus-neutralization assay. Eleven lactating mothers received either Pfizer BNT162b2 (7/11) or Moderna mRNA-1273 (4/11) vaccine primary series. IgG and IgA titers increased in serum and breast milk following each dose, peaking 1–4 weeks after series completion. Titers remained significantly elevated for 7–9 months, except for in breast milk IgA which returned to baseline within 1 month. Furthermore, binding antibodies against all included variants were detected in breast milk collected 1–3 weeks after series completion. However, while vaccination induced a strong neutralizing response against ancestral SARS-CoV-2 in serum and more modest response in breast milk, it did not induce neutralizing antibodies against Omicron BA.4/5 in either specimen type. This study demonstrates that maternal COVID-19 mRNA vaccination may enhance immune protection for infants through breast milk via increased IgG- and IgA-binding-and-neutralizing antibodies; although, variant-specific boosters may be required to optimize immune protection.

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Assessment of SARS-CoV-2 neutralizing antibody titers in breastmilk from convalescent and vaccinated mothers

Cited by 2 publications

References 38 publications

Milk antibody response after 3rd COVID-19 vaccine and SARS-CoV-2 infection and implications for infant protection

Milk antibody response after 3rd COVID-19 vaccine and SARS-CoV-2 infection and implications for infant protection

SARS-CoV-2 Antibody Profiles in Maternal Serum and Breast Milk Following mRNA COVID-19 Vaccination: A Longitudinal Prospective Observational Cohort Study

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