Assessment of Salt Tolerance in Transgenic Potato Carrying <i>AtNHX1</i> Gene

Wang, Li; Zhang, Junlian; Wang, Di; Jin-wen, Zhang; Cui, Yansen; Liu, Yuhui; Yang, Hong-Chang; Binyu,

doi:10.2135/cropsci2013.03.0179

Cited by 13 publications

(6 citation statements)

References 22 publications

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“…The PCA method has been used to analyze huge amounts of data and excavate valuable information, and the analytic result provided help for screening tolerant germplasm resources in crop improving programs (Fleta-Soriano and Munne-Bosch, 2016;Mahajan et al, 2013;Noreen and Ashraf, 2009;Ullah et al, 2017;Wang et al, 2013). A comprehensive system that included morphological, physiological, and biochemical components should be established for the precise assessment of stress tolerance in crops.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Variation of Drought Tolerance in Five Actinidia Species

Zhong¹,

Li²,

Bai³

et al. 2018

J. Amer. Soc. Hort. Sci.

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To select resistant germplasm resources and understand the growth and physiological responses of kiwifruit (Actinidia sp.) to drought stress, five species, Actinidia macrosperma (Acma), Actinidia longicarpa (Aclo), Actinidia deliciosa (Acde), Actinidia hemsleyana (Ache), and Actinidia valvata (Acva), were assessed under tissue culture conditions. Rootless seedlings of five species were cultured in a medium containing polyethylene glycol [PEG (formula weight 8000)] to induce drought stress (0%, 5%, 10%, 15%, and 20%). After a 30-day culture, three growth indices [fresh weight (FW), plant height (PLH), and leaf number (LN)] and six physiological indices were determined, and the drought damage index (DDI) was determined. The DDIs of five species increased, and three growth indices decreased with increasing PEG concentrations. The following changes were observed under 20% PEG treatment conditions: superoxide dismutase (SOD) activities increased significantly in Acma, Aclo, and Ache specimens; peroxidase (POX) activities remained stable in Acde, Ache, and Acva specimens; and catalase (CAT) activities increased sharply in Acma and Acva. Furthermore, the results indicated that soluble sugar (SS) content increased slightly in Acma, Aclo, Acde, and Ache but it decreased in Acva specimens. Proline (PRO) content increased significantly in Acma and Acva, and malondialdehyde (MDA) contents tended to increase under drought stress in all five species. Principal component analysis (PCA) results indicated that the order of drought tolerance in the five genotypes examined in this study under tissue culture conditions was as follows: Acma > Acva > Acde > Aclo > Ache. Therefore, we concluded that Acma and Acva are more resilient germplasm resources that represent promising kiwifruit-breeding materials. Furthermore, tolerance to drought stress in these species should be further investigated under orchard conditions.

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Section: Discussionmentioning

confidence: 99%

In Vitro Variation of Drought Tolerance in Five Actinidia Species

Zhong¹,

Li²,

Bai³

et al. 2018

J. Amer. Soc. Hort. Sci.

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“…Detailed methodology was described previously (Wang et al, 2013). In brief, an evaluation index was developed using the following three steps:

Data standardization, where a subordinator function was formed to standardize the measured raw data of each parameter as follows: $\begin{matrix} eqnarrayright center left \\ μ false(X_{i j} false) = \frac{false(X i j - X min false)}{false(X max - X min false)} \end{matrix}$ $\begin{matrix} eqnarrayright center left \\ μ false(X_{i j} false) = false(\frac{X max - X i j}{X max - X min} false), \end{matrix}$ where, Xij is the value of the j th variable (daily gain of plant height, leaf area expansion rate, stem thickness expansion rate, plant tissue water contents, tuber yield per plant, and rate of plant survival to drought treatment.)

…”

Section: Methodsmentioning

confidence: 99%

“…To quantify the degree of drought tolerance, we employed an integrated evaluation index where a cluster analysis was combined with standard deviation coefficient allocation weighted method. Detailed methodology was described previously (Wang et al, 2013 ). In brief, an evaluation index was developed using the following three steps: Data standardization, where a subordinator function was formed to standardize the measured raw data of each parameter as follows:

where, Xij is the value of the j th variable (daily gain of plant height, leaf area expansion rate, stem thickness expansion rate, plant tissue water contents, tuber yield per plant, and rate of plant survival to drought treatment.)…”

Section: Methodsmentioning

confidence: 99%

Roles of Plasmalemma Aquaporin Gene StPIP1 in Enhancing Drought Tolerance in Potato

et al. 2017

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Survival and mortality of plants in response to severe drought may be related to carbon starvation, but little is known about how plasma membrane intrinsic proteins may help alleviate the drought-induced damage. Here, we determined the roles of plasmalemma aquaporin gene in improving plant water status, maintaining carbon accumulation, and thereby enhancing drought tolerance. Seven StPIP1 transformed potato (Solanum tuberosum L.) lines (namely T1, T2…T7) were compared with non-transgenic control plant at molecule and whole-plant levels. The relative expression of StPIP1 gene was found in leaves, stems and roots, with the most abundant expression being in the roots. The transgenic lines T6 and T7 had the highest StPIP1 expression, averaging 7.2 times that of the control and the greatest differences occurred 48 h after mannitol osmotic stress treatment. Using an evaluation index to quantifying the degree of drought tolerance, we found that the StPIP1 transgenic lines T6 and T7 had the highest drought tolerance, averaging 8.5 times that of the control. Measured at 30 days in drought stress treatment, the control plant decreased net photosynthetic rate by 33 and 56%, respectively, under moderate and severe stresses; also decreased stomatal conductance by 39 and 65%; and lowered transpiration rate by 49 and 69%, compared to the no-stress treatment, whereas the transgenic lines T6 and T7 maintained a relatively stable level with slight decreases in these properties. The constitutive overexpression of StPIP1 in potato improved plant water use efficiency and increased nonstructural carbohydrate concentration, which helped alleviate carbon starvation and minimized the loss of biomass and tuber yield due to drought stress. We conclude that the expression of StPIPs improves overall water relations in the plant and helps maintain photosynthesis and stomatal conductance; these help minimize carbon starvation and enhance the whole plant tolerance to drought stress.

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“…Although some factors such as CAT activity in pea varieties, root proline content in Plantago maritima, leaf MDA level and SOD activity in soybean, and shoot dry weight in wheat were used to distinguish the salt tolerance (Noreen and Ashraf 2009;Zhang et al 2011;Sharbatkhari et al 2013;Sleimi et al 2015), the limitations of using a single evaluation indicator had been discussed (Mwadzingeni et al 2016). PCA was a comprehensive evaluation method that used widely to assess different resistant germplasm resources (Wang et al 2013). In this study, the salt tolerance of four kiwifruit genotypes were assessed by PCA, and Acva-3 had the strongest tolerance to salt stress.…”

Section: Discussionmentioning

confidence: 99%

Growth and physiological responses of four kiwifruit genotypes to salt stress and resistance evaluation

Zhong

Chen

et al. 2019

Journal of Integrative Agriculture

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In this study, four genotypes (Acva-1, Acva-2, Acva-3 and ZM-2) of Actinidia germplasm resources were grown in different salt concentrations (0, 0.4, 0.8 and 1.2 g L-1 NaCl). The growth, physiological and biochemical indicators were measured, and a graded scale was developed as the salt damage index (SDI) according to different damage symptoms in leaves. The results showed SDI increased gradually, and average number and length of new shoot decreased significantly. Three antioxidant enzymes (superoxide dismutase, peroxidase and catalase) and two osmotic adjustment substances (soluble sugar and proline) showed different changes in old and new leaves of four genotypes. SPAD values exhibited a decreased trend in the whole except in the new leaf of Acva-2. Malonaldehyde contents increased and root activity decreased with the increasing salt concentrations. Principal component analysis was used to assess the salt tolerance, and the results showed Acva-3, from Actinidia valvata Dunn., had the strongest tolerance to salt, and could be a potential resistant resource to the salt-tolerance dedicated rootstock breeding of kiwifruit.

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Assessment of Salt Tolerance in Transgenic Potato Carrying AtNHX1 Gene

Cited by 13 publications

References 22 publications

In Vitro Variation of Drought Tolerance in Five Actinidia Species

In Vitro Variation of Drought Tolerance in Five Actinidia Species

Roles of Plasmalemma Aquaporin Gene StPIP1 in Enhancing Drought Tolerance in Potato

Growth and physiological responses of four kiwifruit genotypes to salt stress and resistance evaluation

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