Assessment of Salivary Pellicle on the Surface of Dental Alloys, in vivo

Abstract

Objectives: The molecular composition of the salivary pellicle adsorbed on the surface of dental alloys is determined by the chemical structure of the alloy. The purpose of this study is to compare the composition of salivary pellicle on two dental alloys, chrome cobalt (Co-Cr) and nickel chromium (Ni-Cr) surfaces.Materials and Methods: Protein profile of 2 hour salivary pellicle on customized Co-Cr and Ni-Cr palatal plates were kept in the mouth of healthy volunteers for two hours. Pellicle was used to compare the parotid and the whole saliva using SDS-PAGE (sodiumdodecylsulphatepoyacrylamide gel electrophoresis) and Western blot analysis.Results: Only a limited number of salivary proteins was observed in both Co-Cr and Ni-Cr pellicle. In addition, there were differences in proteins observed Co-Cr pellicle vs. Ni-Cr pellicle. Conclusion:The result of this study suggest there is selectivity in salivary protein adsorption to Co-Cr and Ni-Cr dental prostheses.