Assessment of Safety of Inhaled PMF Isolated from Camel Urine with Potential Activity against COVID-19

Abstract

PMF is a mixture of compounds extracted from lyophilized camel urine. It has many therapeutic effects including anticancer/antiviral activities. It has also a good safety profile. The aim of this study is to assess PMF safety in mice lung tissue after repeated inhalation. Adult male mice (N=15) were used. Clean surgical pads were used as cage bedding to avoid inhalation of wooden bedding. PMF aqueous solution, low and high doses were provided by whole-body inhalation exposure system, once daily for 3 successive days. At the end of the experiment, blood samples were collected, animals were sacrificed, and both lungs were taken for immunological and histology investigations. PMF inhalation in low and high doses showed normal levels of pro-inflammatory cytokines (TNF alpha and IL6) as well as normal antioxidants (GSH, SOD and catalase) in lung tissues. Whereas PMF inhalation in high doses led to an increasing level of MDA. The histological assessment also showed low or mild changes in both alveoli and bronchioles which are more obvious in the case of a high PMF dose. In conclusion, PMF inhalation either in high or low doses does not likely to induce an immunological inflammatory response in lung tissue. However, high doses may cause some histological changes. meta-analysis', Tropical medicine and infectious disease, 5: 180. Ali, A, F Khorshid, H Abu-Araki, and AM Osman. 2011. 'Tumor lung cancer model for assessing anti-neoplastic effect of PMF in rodents: histopathological study', Trends in Applied Sciences Research, 6: 1214.