Assessment of saccharide fractionation by ultrafiltration and nanofiltration

Abstract

This paper addresses the investigation of the fractionation of saccharide mixtures and saccharide mixtures with calcium using ultrafiltration (UF) and nanofiltration (NF). A set of cellulose acetate membranes covered a wide range of molecular weight cut-off (MWCO) ranging from 250 to 46,000 Da and the total feed concentration of saccharides mixtures varied from 1550 to 4700 ppm with the ratio of the two saccharides-solutes (glucose to raffinose) being kept constant at the value of 1.8. The evolution pattern of the saccharide concentration ratio in the UF/NF permeate streams displayed a dependence on the membrane MWCO, on the total sugar concentration and on the presence of calcium ions. For the highest total sugar content, the membranes with MWCO from 2000 to 7000 Da showed saccharide fractionation capability that was enhanced in the presence of calcium. The Steric Pore Flow Model was used to predict individual solute permeation behaviours and to assess the deviations to steric hindered transport of the solutes in multi-component saccharide solutions. (C)

Tailoring the enzymatic synthesis and nanofiltration fractionation of galacto-oligosaccharides

“…A gap between lactose and GOS-2 observed rejections was observed, enforcing the investigation done by [22] during saccharides fractionation assessment. These authors verified that molecular retention mechanism is not solely based on molecular weight sieving effect of the solvent in binary aqueous solutions.…”

“…Cellulose acetate (CA) membranes were prepared by phase inversion method and further subjected to an annealing treatment [22] for 11 min. The tangential flow filtrations were performed in a cell unit described previously [23] with a slit-type (20 cm × 3 cm × 0.2 cm) feed channel and an effective membrane area of 60 cm 2 .…”

“…Recent studies on the fractionation of saccharides by NF and ultrafiltration (UF) membranes [22], based on similar system parameters, have pointed out possible solvent/solute(s) interactions in the feed solutions that may have a crucial influence on the separation outcome. The authors evidenced a range of membranes MWCO (2000-7000 Da) for best effectiveness of sugars fractionation, although at low total concentration of sugars.…”

Continuous Membrane Bioreactor (CMBR) to Produce Nanoparticles from Milk Components

“…According to Gosling et al, (2010), up to 88% of the di-and oligosaccharides were recovered from a commercial GOS mixture using a nanofiltration membrane with only 19% of the monosaccharides remaining in the retentate stream (Goulas et al, 2003). Catarino et al, (2008) investigated the fractionation of saccharide mixtures with calcium using ultrafiltration and nanofiltration processes and reported that saccharide fractionation was enhanced in the presence of calcium. Feng et al, (2009) reported the separation of sugar solutions in total recycle mode operations using nanofiltration membranes and the process resulted in oligosaccharides yield of 70% and purity of 54%.…”

Physicochemical characterization of saccharides powder obtained from yacon roots (Smallanthus sonchifolius) by membrane technology

“…Other nutrients are reported to be low, except for potassium (Lachman et al 2004). FOS extraction from yacon roots may be performed by membrane technology, a potential feasible strategy for industrial manufacture of oligosaccharides (Goulas et al 2002;Kuhn et al 2010) that have been used successfully for their purification (Minhalma et al 2006;Nabarlatz et al 2007;Sjöman et al 2007;Catarino et al 2008). Commercializing FOS in the powder form has the advantage of facilitating manipulation, transport, storage and consumption.…”