Assessment of resistance to root-lesion and root-knot nematodes in Australian hybrid clones of sugarcane and its wild relatives

Cytogenetic and agronomic characterization of intergeneric hybrids between Saccharum spp. hybrid and Erianthus arundinaceus

“…Identification of genuine intergeneric hybrids based on 5S rDNA 3 is highly efficient, and is therefore routinely used in sugarcane breeding programs around the world 15,27 . However, in this study, intergeneric hybrids belonging to Group C exhibited morphological characteristics similar to most hybrids in the remaining two Groups, despite lacking Erianthus 5S rDNA detection.…”

“…In wheat × rye intergeneric hybridization, disease resistance genes from rye were introduced into wheat; however, the resulting breadmaking quality of the hybrids was inferior to that of wheat 25,26 . Various beneficial traits of Erianthus are thought to be introgressed into intergeneric hybrids, such as a high polyphenol content in the roots 15 , nematode resistance 27 and drought tolerance 28 . However, information on the potentially valuable agronomic characteristics of intergeneric F 1 populations between Saccharum and E .…”

Evaluation of root-lesion nematode (Pratylenchus zeae) resistance assays for sugarcane accession lines

“…Details of trial procedures and design of experiments were described elsewhere (Bhuiyan and Garlick, 2021;Bhuiyan et al, 2014;Croft et al, 2015). In short, all accession lines were collected from the SRA germplasm collection, situated at Meringa regional station, Queensland.…”

“…The first component of that work was to develop methods for screening large numbers of sugarcane lines against root-lesion nematodes. Initial research by the authors determined trial conditions, suitable potting media, and extraction methods for root-lesion nematodes (Bhuiyan et al, 2014). The other important aspect was to formulate an assessment method for nematode resistance that is reliable and suitable for screening large numbers of progeny lines.…”

“…In Australia, the resistance of sugarcane accession lines is measured by determining the ability of the root-lesion nematode to reproduce in the roots of the test lines. The nematode reproduction is measured by the number of nematodes per plant at the harvest that has been inoculated with a certain number of nematodes at planting (Bhuiyan et al, 2016;Stirling et al, 2011). The relationships of the number of rootlesion nematodes per plant and its impact on root or shoot biomass were poorly understood.…”