Assessment of Renal Fibrosis with Diffusion-weighted MR Imaging: Study with Murine Model of Unilateral Ureteral Obstruction

Abstract

An ADC decrease in renal fibrosis is associated with an increased number of cells, including fibroblasts. ADC has the potential to serve as a sensitive noninvasive biomarker of renal fibrosis.

Klotho Inhibits Transforming Growth Factor-β1 (TGF-β1) Signaling and Suppresses Renal Fibrosis and Cancer Metastasis in Mice

“…1A) (27). The average volume of pelvis was not different between Klotho-treated and vehicle-treated mice (Fig.…”

“…Magnetic Resonance Imaging (MRI)-T2-weighed images and ADC maps were generated using a 7-T small-animal MR system (Varian, Palo Alto, CA) as previously described (27).…”

“…A part of the kidney was fixed in buffered 10% formalin and processed for standard paraffin sections. The sections were stained with hematoxylin-eosin for cell density measurement as previously described (27). The sections were also stained with Masson's trichrome to detect fibrotic area as blue staining, which was quantified by manually tracing the blue area in 5 randomly selected microscopic fields as in cell density measurements.…”

Renal Relevant Radiology

“…Decreases in ADC have been reported in RCC, although inflammatory lesions impose similar diffusion restrictions (19). A lower ADC might be consequent to increased cellularity that imposes barriers to free diffusion of water (19) or to accumulation of fibroblasts (20).…”

Renal Allograft Fibrosis: Biology and Therapeutic Targets

“…From personal experience, we know that kidney stiffness is much less altered compared to the liver during fibrosis, suggesting that elastography in kidneys might not be sensitive enough for assessment of fibrosis. A third approach suggested to measure renal fibrosis is diffusion-weighted MRI (427)(428)(429)(430)(431)(432)(433)(434)(435)(436)(437). Presently it is unclear whether any of these modalities specifically reflects renal fibrosis.…”