Assessment of recent alcohol consumption by detecting ethyl glucuronide and ethyl sulphate level among traffic accident patients

Dengiz, Huseyin; Dağlioğlu, Nebīle; Gören, İsmail Ethem

doi:10.1080/15389588.2020.1767777

Cited by 2 publications

(1 citation statement)

References 22 publications

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“…The main advantages of this sample material are that compounds with a relatively short lifetime in blood, but with a strong correlation to alcohol consumption, can be entrapped in the hair and are detectable for a longer time (also for years depending on the length of the hair) and at a relatively high concentration [ 64 , 65 ]. In particular, EtG and ethyl sulfate (EtS) are two non-oxidative ethanol metabolites ( Figure 1 ) secreted by the liver which are mainly used as markers of alcohol intake related to incidents [ 66 , 67 , 68 , 69 , 70 , 71 ]. These two markers for recent alcohol intake can be detected in the blood for approximately 10 h after a small to moderate alcohol intake and up to 5 days after large and repeated alcohol intakes [ 67 , 68 , 69 ].…”

Section: Head and Neck Cancer And Alcoholmentioning

confidence: 99%

Alcohol and Head and Neck Cancer: Updates on the Role of Oxidative Stress, Genetic, Epigenetics, Oral Microbiota, Antioxidants, and Alkylating Agents

et al. 2022

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Head and neck cancer (HNC) concerns more than 890,000 patients worldwide annually and is associated with the advanced stage at presentation and heavy outcomes. Alcohol drinking, together with tobacco smoking, and human papillomavirus infection are the main recognized risk factors. The tumorigenesis of HNC represents an intricate sequential process that implicates a gradual acquisition of genetic and epigenetics alterations targeting crucial pathways regulating cell growth, motility, and stromal interactions. Tumor microenvironment and growth factors also play a major role in HNC. Alcohol toxicity is caused both directly by ethanol and indirectly by its metabolic products, with the involvement of the oral microbiota and oxidative stress; alcohol might enhance the exposure of epithelial cells to carcinogens, causing epigenetic modifications, DNA damage, and inaccurate DNA repair with the formation of DNA adducts. Long-term markers of alcohol consumption, especially those detected in the hair, may provide crucial information on the real alcohol drinking of HNC patients. Strategies for prevention could include food supplements as polyphenols, and alkylating drugs as therapy that play a key role in HNC management. Indeed, polyphenols throughout their antioxidant and anti-inflammatory actions may counteract or limit the toxic effect of alcohol whereas alkylating agents inhibiting cancer cells’ growth could reduce the carcinogenic damage induced by alcohol. Despite the established association between alcohol and HNC, a concerning pattern of alcohol consumption in survivors of HNC has been shown. It is of primary importance to increase the awareness of cancer risks associated with alcohol consumption, both in oncologic patients and the general population, to provide advice for reducing HNC prevalence and complications.

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Section: Head and Neck Cancer And Alcoholmentioning

confidence: 99%

Alcohol and Head and Neck Cancer: Updates on the Role of Oxidative Stress, Genetic, Epigenetics, Oral Microbiota, Antioxidants, and Alkylating Agents

et al. 2022

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Determination of Ethyl Glucuronide and Ethyl Sulfate in Dried Blood Spots by UHPLC–MS-MS: Method Validation and Assessment of Ethanol Exposure in Postmortem Samples from Road Traffic Victims

Tegner

Ott

Guterres

et al. 2022

Journal of Analytical Toxicology

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The determination of ethyl glucuronide (EtG) and ethyl sulfate (EtS) in blood has been proposed in clinical and forensic applications to identify recent alcohol consumption. Also, there is a growing interest on the use of dried blood spots (DBS) in toxicological analysis, allowing increased stability of the analytes, and simplifying sample transportation and storage. This study presents the development and validation of a method for quantifying EtG and EtS in DBS by ultra-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS-MS). The DBS samples were extracted with a mixture of methanol and acetonitrile (80:20 v/v), and analyzed by UHPLC–MS-MS with electrospray source in negative mode, after separation with a fluoro-phenyl stationary phase. Validation was performed according to SWGTOX guidelines, with calibration ranges from 0.10 to 18 µg/mL for EtG and 0.02 to 6 µg/mL for EtS. The analytes were stable in DBS stored from –20 and 45°C for 21 days. The method was successfully applied to capillary and venous DBS samples from 20 volunteers after ethanol ingestion and to DBS samples from 99 fatal victims of road traffic injuries. Capillary DBS was comparable to venous DBS and fresh whole blood in Passing Bablok and Bland Altman analysis, with correlation coefficient > 0.91 (p < 0.001) for all comparisons. In postmortem application, the DBS EtG and EtS analysis indicated positive exposure to ethanol in 72.7% of the cases (EtG: 0.10 to 24.0 µg/mL and EtS: 0.03 to 4.11). The identification of ethanol consumption from blood alcohol concentrations (BAC) and EtG/EtS in DBS was in agreement in 98.6% of positive and 96.3% of negative cases (kappa 0.877, p < 0.001), indicating a high level of concordance with BAC in assessing alcohol use in postmortem samples.

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Assessment of recent alcohol consumption by detecting ethyl glucuronide and ethyl sulphate level among traffic accident patients

Cited by 2 publications

References 22 publications

Alcohol and Head and Neck Cancer: Updates on the Role of Oxidative Stress, Genetic, Epigenetics, Oral Microbiota, Antioxidants, and Alkylating Agents

Alcohol and Head and Neck Cancer: Updates on the Role of Oxidative Stress, Genetic, Epigenetics, Oral Microbiota, Antioxidants, and Alkylating Agents

Determination of Ethyl Glucuronide and Ethyl Sulfate in Dried Blood Spots by UHPLC–MS-MS: Method Validation and Assessment of Ethanol Exposure in Postmortem Samples from Road Traffic Victims

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