Assessment of real-time PCR as a method for determining the presence of Verticillium dahliae in different Solanaceae cultivars

Gayoso, Carmen; Ilárduya, Oscar Martı́nez de; Pomar, Federico; Cáceres, Fuencisla Merino de

doi:10.1007/s10658-007-9134-8

Cited by 51 publications

(30 citation statements)

References 29 publications

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“…Difference in the degree of aggressiveness among the different isolates suggested high variability among sampling populations independent of their original location. When applied real-time PCR techniques for detecting fungal mycelium in infected pepper, Gayoso et al (2007) found good correlation between the severity of the symptoms displayed by Verticillium-infected plants and the relative amount of fungal DNA measured as fungus/plant DNA ratio.…”

Section: Growth Physiology Metabolism and Fruit Yield Of Pepper Plamentioning

confidence: 99%

Arbuscular mycorrhizal fungi (AMF) as bioprotector agents against wilt induced by Verticillium spp. in pepper: a review

Goicoechea¹,

Garmendia²,

Sánchez‐Díaz³

et al. 2013

Span. j. agric. res.

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Verticillium dahliae Kleb. is a vascular pathogen that alters water status and growth of pepper plants and causes drastic reductions in yield. Its control is difficult because it can survive in field soil for several years. The application of arbuscular mycorrhizal fungi (AMF) as bioprotector agents against V. dahliae is an alternative to the use of chemicals which, in addition, is more respectful with the environment. The establishment of the mutualistic association of plant roots and AMF involves a continuous cellular and molecular dialogue between both symbionts that includes the preactivation of plant defense responses that may enhance the resistance or tolerance of mycorrhizal plants to soil-borne pathogens. Some AMF can improve the resistance of Capsicum annuum L. against V. dahliae. This is especially relevant for pepper cultivars (i.e. cv. Piquillo) that exhibit high susceptibility to this pathogen. Compared with non-mycorrhizal plants, mycorrhizal pepper can exhibit more balanced antioxidant metabolism in leaves along the first month after pathogen inoculation, which may contribute to delay both the development of disease symptoms and the decrease of photosynthesis in Verticillium-inoculated plants with the subsequent benefit for yield. In stems, mycorrhizal pepper show earlier and higher deposition of lignin in xylem vessels than nonmycorrhizal plants, even in absence of the pathogen. Moreover, AMF can induce new isoforms of acidic chitinases and superoxide dismutase in roots. Mycorrhizal-specif ic induction of these enzymatic activities together with enhanced peroxidase and phenylalanine ammonia-lyase in roots may also be involved in the bioprotection of Verticillium-induced wilt in pepper by AMF.Additional key words: biological control, Capsicum annuum, mycorrhizal symbiosis, Verticillium dahliae Kleb. ResumenRevisión. Los hongos micorrícico arbusculares (MA) como agentes bioprotectores del pimiento frente a la seca inducida por Verticillium spp.Verticillium dahliae Kleb. altera el estado hídrico y el crecimiento de las plantas de pimiento y reduce la producción del fruto. El empleo de hongos micorrícico arbusculares (MA) como bioprotectores contra V. dahliae es una alternativa al uso de productos químicos más respetuosa para el medio ambiente. La asociación entre las raí-ces de las plantas y los hongos MA conlleva un diálogo celular y molecular entre ambos simbiontes, que incluye la preactivación de respuestas de defensa en la planta, lo cual puede incrementar la resistencia o tolerancia de las plantas micorrizadas hacia los patógenos edáficos. Algunos hongos MA incrementan la resistencia del pimiento (Capsicum annuum L.) contra V. dahliae. Esto es especialmente relevante cuando el cultivar (cv. Piquillo, por ejemplo) es muy susceptible hacia el patógeno. En comparación con las plantas no micorrizadas, las hojas de las plantas de pimiento micorrizadas muestran un metabolismo antioxidante más equilibrado a lo largo del primer mes tras la inoculación del patógeno, lo que puede retrasar el desc...

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Section: Growth Physiology Metabolism and Fruit Yield Of Pepper Plamentioning

confidence: 99%

Arbuscular mycorrhizal fungi (AMF) as bioprotector agents against wilt induced by Verticillium spp. in pepper: a review

Goicoechea¹,

Garmendia²,

Sánchez‐Díaz³

et al. 2013

Span. j. agric. res.

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“…It is more accurate and less time consuming than conventional, end-point quantitative PCR because it monitors PCR products before reaction components become limiting. Currently, RT-qPCR technology is commonly used in plant pathology for the precise detection and quantification of pathogens in infected plants and infested soils (reviewed by Schena et al 2004b;Ma and Michailides 2007), and it has been used to quantify V. dahliae DNA as well (Schena et al 2004a;Atallah et al 2007;Gayoso et al 2007). RTqPCR has also been successfully implemented to quantify the biomass of V. dahliae D and ND pathotypes in infected olive plants (Mercado-Blanco et al 2003a;Markakis et al 2009).…”

Section: Pre-planting Measures To Control Vwomentioning

confidence: 99%

Verticillium wilt of olive: a case study to implement an integrated strategy to control a soil-borne pathogen

2010

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Olive (Olea europaea L.) is one of the first domesticated and cultivated tree species and has historical, social and economical relevance. However, its future as a strategic commodity in Mediterranean agriculture is threatened by diverse biotic (traditional and new/emerging pests and diseases) and abiotic (erosion, climate change) menaces. These problems could also be of relevance for new geographical areas where olive cultivation is not traditional but is increasingly spreading (i.e., South America, Australia, etc). One of the major constraints for olive cultivation is Verticillium wilt, a vascular disease caused by the soil-borne fungus Verticillium dahliae Kleb. In this review we describe how Verticillium wilt of olive (VWO) has become a major problem for olive cultivation during the last two decades. Similar to other vascular diseases, VWO is difficult to manage and single control measure are mostly ineffective. Therefore, an integrated disease management strategy that fits modern sustainable agriculture criteria must be implemented. Multidisciplinary research efforts and advances to understand this pathosystem and to develop appropriate control measures are summarized. The main conclusion is that a holistic approach is the best strategy to effectively control VWO, integrating biological, chemical, physical, and cultural approaches.

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“…Pérez-Artés et al 2005;DeBode and Van Poucke 2011;Bilodeau et al 2012) as well as for detection in plant samples (e.g. Schena et al 2004;Karajeh and Masoud 2006;Gayoso et al 2007). So far, however, these protocols have not been developed into procedures for routine screening of planting stock or fields to be planted with crops susceptible to Verticillium wilt.…”

Section: Genetic Diversity and Detection Of V Dahliaementioning

confidence: 99%

Verticillium wilt caused by Verticillium dahliae in woody plants with emphasis on olive and shade trees

2017

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Olive plantations and tree nurseries are economically and ecologically important agricultural sectors. However, Verticillium wilt, caused by Verticillium dahliae Kleb., is a serious problem in olive-growing regions and in tree nurseries worldwide. In this review we describe common and differentiating aspects of Verticillium wilts in some of the main economically woody hosts. The establishment of new planting sites on infested soils, the use of infected plant material and the spread of highly virulent pathogen isolates are the main reasons of increasing problems with Verticillium wilt in tree cultivation. Therefore, protocols for quick and efficient screening of new planting sites as well as planting material for V. dahliae prior to cultivation is an important measure to control Verticillium wilt disease. Furthermore, screening for resistant genotypes that can be included in breeding programs to increase resistance to Verticillium wilt is an important strategy for future disease control. Collectively, these strategies are essential tools in an integrated disease management strategy to control Verticillium wilt in tree plantations and nurseries.

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Assessment of real-time PCR as a method for determining the presence of Verticillium dahliae in different Solanaceae cultivars

Cited by 51 publications

References 29 publications

Arbuscular mycorrhizal fungi (AMF) as bioprotector agents against wilt induced by Verticillium spp. in pepper: a review

Arbuscular mycorrhizal fungi (AMF) as bioprotector agents against wilt induced by Verticillium spp. in pepper: a review

Verticillium wilt of olive: a case study to implement an integrated strategy to control a soil-borne pathogen

Verticillium wilt caused by Verticillium dahliae in woody plants with emphasis on olive and shade trees

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