Assessment of reactivity of three treponemal tests in non-treponemal non-reactive cases from sexually transmitted diseases clinic, antenatal clinic, integrated counselling and testing centre, other different outdoor patient departments/indoor patients of a tertiary care centre and peripheral health clinic attendees

Bala, Manju; Singh, Vikram; Muralidhar, Sumathi; Ramesh, V.

doi:10.4103/0255-0857.115638

Cited by 12 publications

(11 citation statements)

References 8 publications

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“…[ 7 ] However, many centers for sexually transmitted infections (STIs) in India perform only a single screening assay either VDRL or (rapid plasma reagin) RPR for serodiagnosis of syphilis. [ 8 ] Keeping above facts in mind, this study was undertaken to determine the seroprevalence of syphilis by VDRL test and to assess the biological false positivity rate in different patient groups from a tertiary care center in North India.…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence of syphilis by VDRL test and biological false positive reactions in different patient populations: Is it alarming? Our experience from a tertiary care center in India

Patwardhan

Bhattar

Rawat

2020

Indian J Sex Transm Dis

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Introduction: Many centers for sexually transmitted infections in India perform only a single screening assay for diagnosis of syphilis which may yield biological false positive (BFP) reactions. Aims and Objective: The aim of this study was to determine the true picture of seroprevalence of syphilis and BFP reactions in different patient groups. Materials and Methods: A total of 57,308 serial serum samples obtained over a period of 5 years from different patient groups were screened by venereal disease research laboratory (VDRL) test both qualitatively and quantitatively. VDRL reactive sera were confirmed by Treponema pallidum hemagglutination (TPHA) test. Results: The overall seroprevalence of syphilis by VDRL test was 1.27%, and BFP rate in test population was 0.14%. The rate of BFP reactions among total tested male (0.44%) and female (0.1%) patients differs significantly. Out of 733 VDRL reactive samples, 81 were BFP, i.e., BFP reaction is occurring at a frequency of 11% of the total VDRL reactive samples (ratio of 8:1 for true positives/BFP). Similarly, among antenatal cases, almost 24% of the total VDRL reactive samples were BFP, or for every 116 true positives, there were 37 (almost one-third) BFP. Conclusion: Although the overall seroprevalence of syphilis is low; the frequency of occurrence of BFP reactions is quite alarming. Hence, treponemal test must be used for confirmation of VDRL reactive sera.

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Section: Introductionmentioning

confidence: 99%

Seroprevalence of syphilis by VDRL test and biological false positive reactions in different patient populations: Is it alarming? Our experience from a tertiary care center in India

Patwardhan

Bhattar

Rawat

2020

Indian J Sex Transm Dis

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“…However, another study reported seroprevalence of syphilis to be 0.3% among Antenatal cases. 12 Other studies done from various parts of India which showed similar seropositivity among ANC cases were depicted in Table 5. 1,7,11,[13][14][15]…”

Section: Figure 3: Distribution Of Pregnant Females According To Theimentioning

confidence: 89%

Seroprevalence of syphilis among pregnant females attending antenatal clinic at a tertiary care hospital in North India

et al. 2020

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Background: Syphilis is a sexually transmitted disease caused by bacteria named Treponema palladium, subsp. pallidum. Nearly 1.36 million pregnant women are known to be affected by syphilis in the developing countries. When left untreated syphilis during pregnancy can result in adverse fetal outcomes such as spontaneous abortion and stillbirth. Objective of the study was present study was done to evaluate the seroprevalence of syphilis among pregnant females attending Antenatal Clinic (ANC).Methods: A hospital based cross-sectional study was done over a period of six months from January to June 2019. A total of 132 pregnant females were included in the study who attended Antenatal Clinic for routine checkup whose blood samples were sent to Microbiology Laboratory for screening for syphilis by Rapid Plasma Reagin (RPR) test.Results: Out of 132 samples of pregnant females screened for syphilis, none of the sample was found reactive for syphilis by RPR test. Maximum patients (57.6%) belonged to age group 20-25 years followed by (27.3%) of 26-30 years. 30.3% patients belonged to urban areas and 69.7% patients belonged to rural areas. Majority of patients (80.3%) belonged to first trimester, followed by (18.9%) patients to second trimester and (0.8%) patients to third trimester. 78.8% patients were from lower socio-economic class followed by 12.9% patients from middle class and 8.3% patients from upper class.Conclusions: Although zero percent syphilis prevalence was observed in this study, it is recommended that free screening for syphilis should be offered to all pregnant females visiting Antenatal Clinic.

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“…[12] A much lower seroprevalance of 0.3% was reported from an Indian study that evaluated the performance of three treponemal tests in VDRL/RPR nonreactive sera, within the context of a National Reference Laboratory for STIs. [13] Another study on antenatal mothers reported 8.5% active syphilis, 3.3% old or treated cases, 1% biological false positives, and 0.04% primary syphilis on the basis of RPR and TPHA test results. [14]…”

Section: Discussionmentioning

confidence: 99%

Utility of immunochromatographic assay as a rapid point of care test for screening of antenatal syphilis

Kashyap

Sagar

2015

Indian J Sex Transm Dis

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Background and Objectives:Syphilis is one of the most common preventable causes of adverse effects during pregnancy. Antenatal screening prevents the delay between diagnosis and treatment there by reducing the risk of congenital syphilis. The objective of this study was to evaluate the utility of an immunochromatographic assay as a point of care test for antenatal screening of syphilis.Materials and Methods:Sera of 200 antenatal mothers were evaluated for serodiagnosis of syphilis by the venereal disease research laboratory (VDRL), Treponema pallidum hemagglutination assay (TPHA) and SD BIOLINE Syphilis 3.0 test. The performance of SD BIOLINE Syphilis 3.0 test was compared with VDRL as screening assay and TPHA as a confirmatory test.Results:The antenatal prevalence of syphilis was found to be 2% by both VDRL and TPHA. The sensitivity, specificity, positive predictive value, and the negative predictive value of SD BIOLINE Syphilis 3.0 test were 75%, 100%, 100%, and 99.45%, respectively.Conclusions:Antenatal screening and treatment of maternal syphilis are cost-effective health interventions even under the low prevalence of infection. SD BIOLINE Syphilis 3.0 test, although having less sensitivity than the existing testing strategy, can have a tremendous impact on the disease burden if used prudently for the screening of antenatal mothers in peripheral health settings.

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Cited by 12 publications

References 8 publications

Seroprevalence of syphilis by VDRL test and biological false positive reactions in different patient populations: Is it alarming? Our experience from a tertiary care center in India

Seroprevalence of syphilis by VDRL test and biological false positive reactions in different patient populations: Is it alarming? Our experience from a tertiary care center in India

Seroprevalence of syphilis among pregnant females attending antenatal clinic at a tertiary care hospital in North India

Utility of immunochromatographic assay as a rapid point of care test for screening of antenatal syphilis

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