Assessment of RAPD Markers to Analyse the Genetic Diversity among Sunflower (Helianthus annuus L.) Genotypes

Abstract

Genetic diversity estimation among different species is an important tool for genetic improvement to maximize the yield, desirable quality, wider adaptation, pest and insect resistance that ultimately boosting traditional plant breeding methods. The most efficient way of diversity estimation is application of molecular markers. In this study, twenty random amplified polymorphic DNA (RAPD) primers were utilized to estimate the genetic diversity between ten sunflower genotypes. Overall 227 bands were amplified by 20 primers with an average of 11.35 bands per primer. RAPD data showed 86.34% polymorophic bands and 13.65% of monomorophic bands. Genetic similarity was ranged from 50.22% to 87.22%. The lowest similarity (50.22%) was observed between FH-352 and FH-359 and the maximum similarity 87.22% was observed between A-23 and G-46. Polymorphic information content (PIC) values were varying from 0.05 to 0.12 with a mean of 0.09. Cluster analysis based on RAPD results displayed two major distinct groups 1 and 2. Group-2 contains FH-352 which was the most diverse genotype, while group-1 consists of few sub groups with all other genotypes. Ample diversity was found in all the genotypes. Present study reveals novel information about sunflower genome which can be used in future studies for sunflower improvement.

Impact of Climate Change on Crops Adaptation and Strategies to Tackle Its Outcome: A Review

“…Genetic divergence analysis is used for polymorphism, inbreeding, assessment, assortment, and recombination to attain plant perfection, and is amongst the main aspects for defining accomplished inbreeding. Genetic divergence analysis is considered a very important method for the development of new cultivars based on genetic distance and similarities [ 120 , 121 ]. For genetic studies landraces are a significant source, for example, a wheat landrace kept in data bank comprises broader genetic variance and is a valuable basis for stress resistance as it contains cultivars adjustable to diverse environmental stress [ 122 ].…”

Genetic diversity assessment in selected genetic resources of Gmelina arborea Roxb using RAPD markers

“…Umesh Kanna (2001) also observed the same trend in Madhuca latifolia. Raza et al (2018) [32] reported that, the 86.34% polymorphism and 13.65% monomorphism was observed using twenty RAPD markers to estimate the genetic diversity among ten sunflower genotypes. All the accessions originated from the CPTs selected in Tamil Nadu were grouped into cluster-I.…”

Application of RAPD Parameters to Estimate the Genetic Dimension of Sunflower Genotypes

“…To the high proportion of variance between 14 genetic types of sunflower. The results reached by [25] indicated that when they studied 10 strains of sunflower using RAPD indicators, 20 primers were used to know the genetic changes and kinship between strains and through the analysis based on the results of RAPD, which included the strains in two main groups.…”