Assessment of protein release kinetics, stability and protein polymer interaction of lysozyme encapsulated poly(?,?-lactide-co-glycolide) microspheres

Ge, Jingping; Woo, Byung Ho; Kang, Feirong; Singh, Jagdish; DeLuca, Patrick P.

doi:10.1016/s0168-3659(01)00533-8

Cited by 137 publications

(87 citation statements)

References 30 publications

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“…It seems that the delivery of therapeutic proteins over a prolonged period is within reach. Reprinted from (Jiang et al, 2002), with permission from Elsevier. and from microparticles prepared by a s/o/w method after protein colyophilisation with PEG (triangles).…”

Section: Resultsmentioning

confidence: 99%

“…As only a few proteins such as tetanus toxoid (Jung et al, 2002) are stable in these conditions, some authors performed protein stability studies to assess the in vitro release study in an optimal release medium (e.g., acetate, citrate or TRIS-HCl buffers) ( Table 1). For instance, lysozyme, which was found to aggregate in phosphate buffer resulting in incomplete release, was stabilized in glycine buffer and exhibited a complete release (Figure 1) (Jiang et al, 2002).…”

Section: Influence Of the Release Mediummentioning

confidence: 99%

“…They attributed this slow release to protein aggregation in the polymer matrix. In contrast, a faster and complete release of Insulin-like Growth Factor-I (IGF-1) (Lam et al, 2000) and lysozyme (Jiang et al, 2002) was observed at acidic pH levels (pH 5.4 and pH 2.5, respectively), where these proteins are not prone to aggregation.…”

Section: Influence Of the Release Mediummentioning

confidence: 99%

“…Moreover, despite the use of a 'non' physiological buffer (such as acetate pH 5), the in vitro release allowed good in vitro-in vivo correlations (Figure 1) (Jiang et al, 2002;Jiang et al, 2003). In the second case, it was supposed that a physiological buffer could elicit better information on the in vivo release profile of the protein.…”

Section: Influence Of the Release Mediummentioning

confidence: 99%

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How to achieve sustained and complete protein release from PLGA-based microparticles?

Giteau

Venier-Julienne

Aubert-Pouëssel

et al. 2008

International Journal of Pharmaceutics

234

184

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One of the most challenging tasks in the delivery of therapeutic proteins from PLGAbased microparticles is the sustained and complete release of the protein in its native form.The mechanisms responsible for incomplete protein release from these devices are numerous and complex; the beneficial effect of different formulations has often been evaluated in vitro.Strategies employed for overcoming protein destabilization during the release step are reviewed in this paper. Proteins have been protected in the deleterious environment by adding stabilizers to the formulation, or by modifying the protein or the polymer. Alternatively, some strategies have aimed at avoiding the formation of the destabilizing environment. As experimental conditions may influence the results from in vitro release studies, we initially report precautions to avoid adverse effects.

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Section: Resultsmentioning

confidence: 99%

Section: Influence Of the Release Mediummentioning

confidence: 99%

Section: Influence Of the Release Mediummentioning

confidence: 99%

Section: Influence Of the Release Mediummentioning

confidence: 99%

See 2 more Smart Citations

How to achieve sustained and complete protein release from PLGA-based microparticles?

Giteau

Venier-Julienne

Aubert-Pouëssel

et al. 2008

International Journal of Pharmaceutics

234

184

View full text Add to dashboard Cite

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“…The "burst effect" is often seen as controversy as to whether this is due to near-surface entrapped drug or surface-absorbed drug [36]. The initial burst and release of drug from the nanotubes may be related to several factors including 1) high relative top surface area 2) increased drug diffusivity through tube walls/channels and 3) high porosity.…”

Section: Resultsmentioning

confidence: 99%

A New Nano-Platform for Drug Release via Nanotubular Aluminum Oxide

Noh¹,

Brammer²,

Choi³

et al. 2011

JBNB

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Enzyme‐Coated Mesoporous Silica Nanoparticles as Efficient Antibacterial Agents In Vivo

Wang

2013

Adv Healthcare Materials

128

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Despite the fact that pathogenic infections are widely treated by antibiotics in the clinic nowadays, the increasing risk of multidrug-resistance associated with abuse of antibiotics is becoming a major concern in global public health. The increased death toll caused by pathogenic bacterial infection calls for effective antibiotic alternatives. Lysozyme-coated mesoporous silica nanoparticles (MSNs⊂Lys) are reported as antibacterial agents that exhibit efficient antibacterial activity both in vitro and in vivo with low cytotoxicity and negligible hemolytic side effect. The Lys corona provides multivalent interaction between MSNs⊂Lys and bacterial walls and consequently raises the local concentration of Lys on the surface of cell walls, which promotes hydrolysis of peptidoglycans and increases membrane-perturbation abilities. The minimal inhibition concentration (MIC) of MSNs⊂Lys is fivefold lower than that of free Lys in vitro. The antibacterial efficacy of MSNs⊂Lys is evaluated in vivo by using an intestine-infected mouse model. Experimental results indicate that the number of bacteria surviving in the colon is three orders of magnitude lower than in the untreated group. These natural antibacterial enzyme-modified nanoparticles open up a new avenue for design and synthesis of next-generation antibacterial agents as alternatives to antibiotics.

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Assessment of protein release kinetics, stability and protein polymer interaction of lysozyme encapsulated poly(?,?-lactide-co-glycolide) microspheres

Cited by 137 publications

References 30 publications

How to achieve sustained and complete protein release from PLGA-based microparticles?

How to achieve sustained and complete protein release from PLGA-based microparticles?

A New Nano-Platform for Drug Release via Nanotubular Aluminum Oxide

Enzyme‐Coated Mesoporous Silica Nanoparticles as Efficient Antibacterial Agents In Vivo

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