Assessment of prestin level changes as an inner-ear biomarker in patients with idiopathic sudden sensorineural hearing loss

Saadat, Farshid; Jalali, Mir Mohammad; Akbari, Maryam

doi:10.1017/s0022215121003108

Cited by 3 publications

(1 citation statement)

References 16 publications

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“…Since OHCs are highly vulnerable to various insults, OHC-specific proteins are considered an excellent choice for revealing biomarkers 2 that are useful in detecting cochlear stress and damage. By using a sandwich enzyme-linked immunosorbent assay (ELISA), Prestin was detected in the bloodstream of humans [13][14][15], rats [16,17], guinea pigs [18,19], and mice [20]. Several reports suggested that Prestin in the bloodstream could be used as a biomarker for hearing loss such as idiopathic sudden sensorineural hearing loss [13-15, 21, 22], noise-induced hearing loss [16,17,[23][24][25], sensory hearing loss [26][27][28], age-related hearing loss [29], ototoxic regents induced hearing loss, such as HPβCD [30] and cisplatin [18][19][20]31], and also hearing loss observed in various diseases like Meniere's Disease and Vestibular Migraine [32], COVID-19 [33], lead poison [34], and even surgical related damage [21].…”

Section: Introductionmentioning

confidence: 99%

Outer Hair Cell Motor Protein, Prestin, Is Not a Reliable Serological Biomarker for Mouse Cochlear Damage

Zheng,

Zhou,

Fuentes

et al. 2024

Preprint

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The motor protein Prestin found in the outer hair cells (OHCs) of the inner ear is responsible for producing high sensitivity and sharp frequency selectivity for mammalian hearing. Some studies have suggested that Prestin could serve as a serological biomarker for cochlear damage since OHCs are highly vulnerable to damage from various sources of insults. However, the reported data are inconsistent and lack appropriate negative controls. To investigate this further, we measured Prestin quantities in the bloodstream using ELISA kits from different companies. Wildtype (WT) mice were exposed to different ototoxic treatments, including noise exposure and ototoxic reagents that kill OHCs rapidly. Prestin-knockout (KO) mice were used as a negative control. Our data show that some ELISA kits were not able to detect Prestin specifically. The ELISA kit that could detect Prestin protein from cochlear homogenates failed to detect Prestin in the bloodstream despite significant damage to OHCs in cochleae. Furthermore, the optical densities of samples, which correlate to Prestin quantities, were significantly influenced by hemolysis in the samples. In conclusion, Prestin from OHCs is not a sensitive and reliable serological biomarker for detecting cochlear damage in mice.

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Section: Introductionmentioning

confidence: 99%

Outer Hair Cell Motor Protein, Prestin, Is Not a Reliable Serological Biomarker for Mouse Cochlear Damage

Zheng,

Zhou,

Fuentes

et al. 2024

Preprint

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Prestin in Human Perilymph, Cerebrospinal Fluid, and Blood as a Biomarker for Hearing Loss

Gadenstaetter,

Krumpoeck,

Auinger

et al. 2024

Otolaryngol.--head neck surg.

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ObjectiveDetermining the concentration of prestin in human blood, cerebrospinal fluid (CSF), and perilymph (PL), and evaluating its suitability as a clinical biomarker for sensori‐neural hearing loss (SNHL).Study DesignHuman blood, CSF, and PL samples were intraoperatively collected from 42 patients with tumors of the internal auditory canal or with intracochlear tumors undergoing translabyrinthine or middle fossa tumor removal. Prestin concentration was measured using enzyme‐linked immunosorbent assay and linear regression analyses were performed to investigate its associations with audiological as well as vestibular test results.SettingTertiary referral center.ResultsThe median prestin concentration in blood samples of the 42 study participants (26 women, mean ± standard deviation age, 52.7 ± 12.5 years) was 1.32 (interquartile range, IQR, 0.71‐1.99) ng/mL. CSF prestin levels were significantly higher with 4.73 (IQR, 2.45‐14.03) ng/mL (P = .005). With 84.74 (IQR, 38.95‐122.00) ng/mL, PL prestin concentration was significantly higher compared to blood (P = .01) and CSF (P = .03) levels. Linear regression analyses showed significant associations of CSF prestin concentration with preoperative hearing levels (pure‐tone average and word recognition; P = .008, R2 = 0.1894; P = .03, R2 = 0.1857), but no correlations with blood or PL levels.Conclusion and RelevanceThis study's findings highlight the volatile nature of prestin levels and provide the first insights into this potential biomarker's concentrations in body fluids apart from blood. Future investigations should comprehensively assess human prestin levels with different etiologies of SNHL, prestin's natural homeostasis and systemic circulation, and its temporal dynamics after cochlear trauma. Finally, clinically approved detection kits for prestin are urgently required prior to considering a potential translational implementation of this diagnostic technique.

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Verification of Outer Hair Cell Motor Protein, Prestin, as a Serological Biomarker for Mouse Cochlear Damage

Zheng,

Zhou,

Fuentes

et al. 2024

IJMS

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The motor protein prestin, found in the inner ear’s outer hair cells (OHCs), is responsible for high sensitivity and sharp frequency selectivity in mammalian hearing. Some studies have suggested that prestin could be a serological biomarker for cochlear damage, as OHCs are highly vulnerable to damage from various sources. However, the reported data are inconsistent and lack appropriate negative controls. To investigate whether prestin can be used as a serological biomarker for cochlear damage or stress, we measured prestin quantities in the bloodstreams of mice using ELISA kits from different companies. Wildtype (WT) mice were exposed to different ototoxic treatments, including noise exposure and ototoxic reagents that rapidly kill OHCs. Prestin-knockout (KO) mice were used as a negative control. Our data show that some ELISA kits were not able to detect prestin specifically. The ELISA kit that could detect the prestin protein from cochlear homogenates failed to detect prestin in the bloodstream, despite there being significant damage to OHCs in the cochleae. Furthermore, the optical densities of the serum samples, which correlate to prestin quantities, were significantly influenced by hemolysis in the samples. In conclusion, Prestin from OHCs is not a sensitive and reliable serological biomarker for detecting cochlear damage in mice using ELISA.

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Assessment of prestin level changes as an inner-ear biomarker in patients with idiopathic sudden sensorineural hearing loss

Cited by 3 publications

References 16 publications

Outer Hair Cell Motor Protein, Prestin, Is Not a Reliable Serological Biomarker for Mouse Cochlear Damage

Outer Hair Cell Motor Protein, Prestin, Is Not a Reliable Serological Biomarker for Mouse Cochlear Damage

Prestin in Human Perilymph, Cerebrospinal Fluid, and Blood as a Biomarker for Hearing Loss

Verification of Outer Hair Cell Motor Protein, Prestin, as a Serological Biomarker for Mouse Cochlear Damage

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