Assessment of polymorphisms in mysostatin gene and their allele substitution effects showed weak association with growth traits in Iranian Markhoz goats

Abstract

SUMMARYPolymorphisms in the myostatin (MSTN) gene were detected in 150 female Iranian Markhoz goats. Two 573 base pairs (bp) and 475 bp fragments of the MSTN gene, which contains a deletion 5 bp indel (206 TTTTA/), in the region of exon 1 encoding the 5′ untranslated region (UTR) of the MSTN transcript, and two single-nucleotide polymorphisms (SNPs) of substitution (339T/A, 169T/G) in exon 1 and 3 regions, respectively, were amplified. The polymerase chain reaction (PCR) products were digested separately using restriction enzyme endonuclease DraI, HinIII and HindIII. The digestion results indicated AA and AB genotypes in the region of exon 1 encoding the 5′UTR of the MSTN transcript, AA, AT and TT genotypes in exon 1 and TT, TG and GG genotypes in exon 3. The SNPs loci were in Hardy–Weinberg disequilibrium but the deletion locus showed equilibrium in the Markhoz goat population. Evaluation of associations between the polymorphisms with the studied growth traits showed that the AA and GG genotypes of exons 1 and 3 have a significant positive effect on weight at 6 months of age (W6) and average daily gain (ADG) traits, but genotypes in the region of exon 1 encoding the 5′UTR of the MSTN transcript did not have any significant effect on the studied growth traits. The statistical analyses showed a positive and significant effect of the 339A allele (exon 1) for W6 and negative and significant effect of the 169G allele (exon 3) for ADG trait. Therefore, these results suggest that the MSTN gene could be a potential candidate gene that affects ADG and W6 traits in goats. More studies are needed to simultaneously consider variants of this region in a larger population to better understand MSTN gene effects on the economic traits in goat.

Myostatin (MSTN) Gene Indel Variation and Its Associations with Body Traits in Shaanbei White Cashmere Goat

“…Numerous studies and researches reported that there were various SNPs and indels loci in both domestic and exotic commercial breeds of goat, sheep, cattle, pig, rabbit, as well as poultry [36]. Additionally, plenty of research focused on a 5 bp indel in 5' UTR of MSTN gene within different goat breeds to uncover its distributions and effects on growth traits of goats [37]. A research detected distributions of the 5 bp indel in Black Bengal, Sirohi, Osmanabadi, Jakhrana, Jamunapari, Barbari, Marwari goat breeds [38].…”

Polymorphic variation and phylogeny of the myostatin gene in wild Punjab Urial (Ovis vignei punjabiensis)

“…The estimated weight of loin primal cuts, chum and hind leg were observed increased significantly[18]. Other previous studies reported three mutations on position number 224bp, 226bp and 242bp in MSTN exon 1 of makuei sheep that were found responsible for increase heart girth and leg circumference[19]…”

A Deletion Downstream of the CHCHD7 Gene Is Associated with Growth Traits in Sheep

“…However, both genotypes (wild-type and deletion genotype) were found in TS, LXBH, STHS, and LFTS sheep. This difference might be due to differences in breeding, including differences in geographic locations of breeds and various environmental factors [ 24 ], or perhaps, the number of individuals with heterozygous deletion genotypes was too small to detect in HS, SS, and AUW sheep. In previous studies, nontarget products were heteroduplexes [ 25 ], and heteroduplexes were only in individuals heterozygous for the mutation [ 26 ].…”