Assessment of plasma aminothiol levels and the association with recurrent atherothrombotic events in patients hospitalized for an acute coronary syndrome: a prospective study

Focks, Jeroen Jaspers; Schaik, A. Van; Clappers, Nick; Dijk, Erica; Oijen, Martijn G.H. van; Verheugt, Freek W.A.; Peters, Wilbert H.M.

doi:10.1515/cclm-2013-0103

Cited by 9 publications

(7 citation statements)

References 26 publications

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“… 29 , 30 This may be because cysteine is independently maintained from homocysteine and represents a more abundant and reactive aminothiol that on oxidation forms cystine, which is 30 times more abundant than homocysteine and perhaps is a more pathological component. Although some studies have shown association between total cysteine and CVD, 31 , 32 none until now has examined the individual oxidized and reduced components or their respective contribution to the oxidant burden. These data may thus offer a partial explanation for the failure of homocysteine targeted therapy and possible new treatment opportunities.…”

Section: Discussionmentioning

confidence: 99%

Novel Biomarker of Oxidative Stress Is Associated With Risk of Death in Patients With Coronary Artery Disease

et al. 2016

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Background Free radical scavengers have failed to improve patient outcomes promoting the concept that clinically important oxidative stress (OS) may be mediated by alternative mechanisms. We sought to examine the association of emerging aminothiol markers of non-free radical mediated oxidative stress with clinical outcomes. Methods and Results Plasma levels of reduced (cysteine and glutathione) and oxidized (cystine and glutathione disulphide) aminothiols were quantified by high performance liquid chromatography in 1411 patients undergoing coronary angiography (mean age 63 years, male 66%). All patients were followed for a mean of 4.7±2.1 years for the primary outcome of all-cause death (n=247). Levels of cystine (oxidized) and glutathione (reduced) were associated with risk of death (p<0.001 both) before and after adjustment for covariates. High cystine and low glutathione levels (>+1 SD & <−1 SD respectively) were associated with higher mortality (adjusted HR 1.63 (95% CI 1.19–2.21; HR 2.19 (95% CI 1.50–3.19), respectively) compared to those outside these thresholds. Furthermore, the ratio of cystine/glutathione was also significantly associated with mortality (adjusted HR 1.92 (95% CI 1.39–2.64) and was independent of and additive to hs-CRP level. Similar associations were found for other outcomes of cardiovascular death and combined death and myocardial infarction. Conclusions A high burden of OS, quantified by the plasma aminothiols, cystine, glutathione and their ratio is associated with mortality in patients with CAD, a finding that is independent of and additive to the inflammatory burden. Importantly, this data supports the emerging role of non-free radical biology in driving clinically important oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Novel Biomarker of Oxidative Stress Is Associated With Risk of Death in Patients With Coronary Artery Disease

et al. 2016

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“…Two prospective studies investigated whether GSH predicts CVD. GSH appeared independently and inversely associated with recurrent atherothrombotic events in a cohort of 375 survivors admitted for an ACS, followed for a median duration of 2.7 years (108). Similarly, in a sample from the Hunter Community Study free of CVEs, 5-year absolute cardiovascular risk score calculated using the Framingham Risk Equation was independently and inversely associated to plasma GSH levels (220).…”

Section: B Direct Antioxidantsmentioning

confidence: 90%

Atherothrombosis and Oxidative Stress: Mechanisms and Management in Elderly

Violi

Carnevale

Pignatelli

et al. 2017

Antioxidants & Redox Signaling

102

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“…The quantification of homocysteine (Hcy) and cysteine (Cys) is of prime importance for biological, clinical, and pharmaceutical research . Hcy is considered a risk factor for complications of cardiovascular diseases .…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis coupled with chloroform‐acetonitrile extraction for rapid and highly selective determination of cysteine and homocysteine levels in human blood plasma and urine

et al. 2017

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A rapid and selective method has been developed for highly sensitive determination of total cysteine and homocysteine levels in human blood plasma and urine by capillary electrophoresis (CE) coupled with liquid–liquid extraction. Analytes were first derivatized with 1,1′‐thiocarbonyldiimidazole and then samples were purified by chloroform–ACN extraction. Electrophoretic separation was performed using 0.1 M phosphate with 30 mM triethanolamine, pH 2, containing 25 μM CTAB, 2.5 μM SDS, and 2.5% polyethylene glycol 600. Samples were injected into the capillary (with total length 32 cm and 50 μm id) at 2250 mbar*s and subsequent injection was performed for 30 s with 0.5 M KОН. The total analysis time was less than 9 min, accuracy was 98%, and precision was <2.6%. The LOD was 0.2 μM for homocysteine and 0.5 μM for cysteine. The use of liquid–liquid extraction allowed the precision and sensitivity of the CE method to be significantly increased. The validated method was applied to determine total cysteine and homocysteine content in human blood plasma and urine samples obtained from healthy volunteers and patients with kidney disorders.

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Assessment of plasma aminothiol levels and the association with recurrent atherothrombotic events in patients hospitalized for an acute coronary syndrome: a prospective study

Cited by 9 publications

References 26 publications

Novel Biomarker of Oxidative Stress Is Associated With Risk of Death in Patients With Coronary Artery Disease

Novel Biomarker of Oxidative Stress Is Associated With Risk of Death in Patients With Coronary Artery Disease

Atherothrombosis and Oxidative Stress: Mechanisms and Management in Elderly

Capillary electrophoresis coupled with chloroform‐acetonitrile extraction for rapid and highly selective determination of cysteine and homocysteine levels in human blood plasma and urine

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