Assessment of photosystem II thermoluminescence as a tool to investigate the effects of dehydration and rehydration on the cyclic/chlororespiratory electron pathways in wheat and barley leaves

Bürling, Kathrin; Ducruet, Jean‐Marc; Cornic, Gabriel; Hunsche, Maurício; Cerović, Zoran G.

doi:10.1016/j.plantsci.2014.03.013

Cited by 8 publications

(20 citation statements)

References 56 publications

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“…In case of slow dehydration, the acclimation avoids the activation of cyclic pathways and preserves the pH gradients even under drought (Bürling et al 2014). Nevertheless, chloroplasts are a major site of damage when plants are exposed to severe stress (Walters 2005).…”

Section: Introductionmentioning

confidence: 99%

Acclimations to light quality on plant and leaf level affect the vulnerability of pepper (Capsicum annuum L.) to water deficit

2015

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We investigated the influence of light quality on the vulnerability of pepper plants to water deficit. For this purpose plants were cultivated either under compact fluorescence lamps (CFL) or light-emitting diodes (LED) providing similar photon fluence rates (95 µmol m(-2) s(-1)) but distinct light quality. CFL emit a wide-band spectrum with dominant peaks in the green and red spectral region, whereas LEDs offer narrow band spectra with dominant peaks at blue (445 nm) and red (665 nm) regions. After one-week acclimation to light conditions plants were exposed to water deficit by withholding irrigation; this period was followed by a one-week regeneration period and a second water deficit cycle. In general, plants grown under CFL suffered more from water deficit than plants grown under LED modules, as indicated by the impairment of the photosynthetic efficiency of PSII, resulting in less biomass accumulation compared to respective control plants. As affected by water shortage, plants grown under CFL had a stronger decrease in the electron transport rate (ETR) and more pronounced increase in heat dissipation (NPQ). The higher amount of blue light suppressed plant growth and biomass formation, and consequently reduced the water demand of plants grown under LEDs. Moreover, pepper plants exposed to high blue light underwent adjustments at chloroplast level (e.g., higher Chl a/Chl b ratio), increasing the photosynthetic performance under the LED spectrum. Differently than expected, stomatal conductance was comparable for water-deficit and control plants in both light conditions during the stress and recovery phases, indicating only minor adjustments at the stomatal level. Our results highlight the potential of the target-use of light quality to induce structural and functional acclimations improving plant performance under stress situations.

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Section: Introductionmentioning

confidence: 99%

Acclimations to light quality on plant and leaf level affect the vulnerability of pepper (Capsicum annuum L.) to water deficit

2015

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“…TL revealed significant differences between ecotypes upon drought-stress occurrence. Drought stress induced a significant shift of B band to lower temperature only in the BG ecotype, indicating that the part of the PSII centers were destabilized by drought in these plants (Peeva and Maslenkova, 2004; Bürling et al, 2014). The higher stability of IT thylakoid membranes positively correlated with increased isoprene emission, which increased only in IT drought-stressed samples.…”

Section: Discussionmentioning

confidence: 98%

“…However, the temperature maximum of B band returned to the value similar to control after re-watering, indicating no permanent impairment of PSII in recovering BG plants. The intensity of the AG band is also often found to increase in dehydrated leaves (Peeva et al, 2012; Bürling et al, 2014). However, AG intensity decreased in drought-stressed BG ecotype, and almost disappeared after re-watering, suggesting low capacity of the biochemical machinery to support the cyclic and chlororespiratory pathways (Bürling et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…The intensity of the AG band is also often found to increase in dehydrated leaves (Peeva et al, 2012; Bürling et al, 2014). However, AG intensity decreased in drought-stressed BG ecotype, and almost disappeared after re-watering, suggesting low capacity of the biochemical machinery to support the cyclic and chlororespiratory pathways (Bürling et al, 2014). On the contrary, no changes in AG band intensity and position were observed in IT leaves.…”

Section: Discussionmentioning

confidence: 99%

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Physiological and structural adjustments of two ecotypes ofPlatanus orientalisL. from different habitats in response to drought and re-watering

Velikova

Tsonev

Tattini

et al. 2018

Conservation Physiology

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“…In fact, the intensity of the AG band is considered as an indicator of a high ATP/ADP and NADPH/NADP + ratio in chloroplast (Krieger et al 1998). A raise in the assimilatory potential (NADPH + ATP) due to stoma closure under slow dehydration has been also correlated with the strong increase in the AG band observed in wheat cultivar (Bürling et al 2014). A decrease in the ratio between the intensity of the AG and B bands (I AG /I B ratio) observed in cold-sensitive pea varieties under cold conditions has also been correlated with a decrease in the assimilatory potential due to a cold-induced phosphate limitation in the chloroplast (Roman and Ducruet 2000).…”

Section: Introductionmentioning

confidence: 97%

The afterglow thermoluminescence band as an indicator of changes in the photorespiratory metabolism of the model legumeLotus japonicus

García‐Calderón

Betti

Márquez

et al. 2019

Physiologia Plantarum

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The afterglow (AG) luminescence is a delayed chlorophyll fluorescence emitted by the photosystem II that seems to reflect the level of assimilatory potential (NADPH+ATP) in chloroplast. In this work, the thermoluminescence (TL) emissions corresponding to the AG band were investigated in plants of the WT and the Ljgln2‐2 photorespiratory mutant from Lotus japonicus grown under either photorespiratory (air) or non‐photorespiratory (high concentration of CO2) conditions. TL glow curves obtained after two flashes induced the strongest overall TL emissions, which could be decomposed in two components: B band (tmax = 27–29°C) and AG band (tmax = 44–45°C). Under photorespiratory conditions, WT plants showed a ratio of 1.17 between the intensity of the AG and B bands (IAG/IB). This ratio increased considerably under non‐photorespiratory conditions (2.12). In contrast, mutant Ljgln2‐2 plants grown under both conditions showed a high IAG/IB ratio, similar to that of WT plants grown under non‐photorespiratory conditions. In addition, high temperature thermoluminescence (HTL) emissions associated to lipid peroxidation were also recorded. WT and Ljgln2‐2 mutant plants grown under photorespiratory conditions showed both a significant HTL band, which increased significantly under non‐photorespiratory conditions. The results of this work indicate that changes in the amplitude of IAG/IB ratio could be used as an in vivo indicator of alteration in the level of photorespiratory metabolism in L. japonicus chloroplasts. Moreover, the HTL results suggest that photorespiration plays some role in the protection of the chloroplast against lipid peroxidation.

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Assessment of photosystem II thermoluminescence as a tool to investigate the effects of dehydration and rehydration on the cyclic/chlororespiratory electron pathways in wheat and barley leaves

Cited by 8 publications

References 56 publications

Acclimations to light quality on plant and leaf level affect the vulnerability of pepper (Capsicum annuum L.) to water deficit

Acclimations to light quality on plant and leaf level affect the vulnerability of pepper (Capsicum annuum L.) to water deficit

Physiological and structural adjustments of two ecotypes ofPlatanus orientalisL. from different habitats in response to drought and re-watering

The afterglow thermoluminescence band as an indicator of changes in the photorespiratory metabolism of the model legumeLotus japonicus

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