Assessment of phagocytic activity in whole blood using laser flow cytometry

Gabrilovich, Dmitry I.; Serebrovskaya, L. V.

doi:10.1016/0022-1759(91)90382-p

Cited by 4 publications

(3 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Those cells were then secondarily labeled with DH59B (VMRD Inc.), which is specific for granulocytes and monocytes, and subsequently with FITC-labeled anti-mouse IgG, to confirm that the R3-gated cells in the FC analysis were granulocytes and monocytes. FC of FITC (FL1) and PI (FL2) mean fluorescence was measured before and after the addition of 50 l of a 0.4% solution of trypan blue (Invitrogen Life Technologies) to each tube in order to quench the fluorescent signals imparted by nonphagocytosed bacteria or by extracellular bacteria that adhere nonspecifically to the surface of the phagocytes (22).…”

Section: Nonspecific Immunostimulator Barodonmentioning

confidence: 99%

Immunostimulatory Effects of the Anionic Alkali Mineral Complex BARODON on Equine Lymphocytes

Koo

Ryu

Ahn

et al. 2006

Clin Vaccine Immunol

View full text Add to dashboard Cite

Previous studies have shown that the anionic alkali mineral complex BARODON has an immunoenhancing effect on pigs as an adjuvant and as a nonspecific immunostimulant. Likewise, the equine immune system has been defined with various monoclonal antibodies specific to equine leukocyte differentiation antigens to determine the possibility of enhancing equine resistance to respiratory diseases and promoting other immunostimulatory effects with the application of BARODON. Compared with the control group, after 3 weeks of treatment, BARODON-treated groups showed higher proportions of cells (P < 0.05) expressing major histocompatibility complex class II and CD2, CD4؉ , CD4 ؉ CD25 ؉ , CD8 ؉ , and CD8 ؉ CD25 ؉ T lymphocytes, dendritic cells, and surface immunoglobulin M ؉ B lymphocytes in peripheral blood, as well as enhanced cell proliferative responses with phytohemagglutinin and increased phagocytic activity against Streptococcus equi and Staphylococcus aureus strains with high antibiotic resistance, the bacteria frequently identified as etiologic agents of equine respiratory diseases at the Seoul Race Park in Seoul, Korea. This study shows that BAR-ODON may act as an immunostimulator and can be an effective alternative to antimicrobial feed additives for nonspecific improvements in equine immune responses, particularly against respiratory diseases.Equine respiratory disease is believed to be the second most important cause of poor performance, interruption of training, and premature retirement among performance horses (41,50,61,62,72). In Seoul, Korea, the Seoul Race Park experienced yearly epizootics of infectious upper respiratory diseases (IURD), with an estimated incidence of 29.6%, from 2001 to 2005. IURD affects the nasal passages and throat region and results in chronic coughing, exercise intolerance, weight loss, and nasal discharge

show abstract

Section: Nonspecific Immunostimulator Barodonmentioning

confidence: 99%

Immunostimulatory Effects of the Anionic Alkali Mineral Complex BARODON on Equine Lymphocytes

Koo

Ryu

Ahn

et al. 2006

Clin Vaccine Immunol

View full text Add to dashboard Cite

show abstract

“…The most frequently used particles in phagocytotic measurements are zymosan (1)(2)(3)(4)(5) and Staphylococcus aureus (1,2,6-9), labeled almost without exception with fluorescein isothiocyanate (FITC). FITC-labeled Streptococcus pneumoniae (10,11), Escherichia coli (12), Neisseria meningitis (13), latex particles (5,12,14,15), herpes simplex virus (16,17), and entire yeasts such as Saccharomyces cerevisiae (18)(19)(20), and Candida albicans (8,21,22) have also been used. In this study, zymosan was used as a phagocytosable particle.…”

mentioning

confidence: 99%

“…Phagocytotic methods presented in literature are frequently characterized by a high phagocytotic concentration in a low reaction volume (usually 2-5 3 10 6 /ml in 0.1-0.25 ml) (5,9,13,15,17,24,26,27). Another distinctive feature of many phagocytotic methods presented in the literature is that they often use only a single measuring time point instead of kinetic measurement (4)(5)(6)(7)10,11,13,17,(20)(21)(22)24,28,29). Opsonization of phagocytosable particles has been performed without exception by adding serum or plasma (5-20%) into a reaction mixture.…”

mentioning

confidence: 99%

Flow cytometric quantitative determination of ingestion by phagocytes needs the distinguishing of overlapping populations of binding and ingesting cells

2005

View full text Add to dashboard Cite

Background: The use of flow cytometry with fluorescently labeled particles provides the means to examine quantitatively the phagocytotic capacity of an individual phagocyte. This report describes an improved flow cytometric method of analysis for kinetic measurement of phagocytosis of fluorescein isothiocyanate (FITC)-labeled zymosan particles by human leukocytes. Methods: FITC-labeled zymosan was incubated with leukocyte suspension, and at selected time intervals fluorescence positive neutrophils were divided by phagocytotic gates into three subpopulations: neutrophils that were neither binding nor ingesting particles, neutrophils that were only binding particles (binding cells), and neutrophils that were binding and ingesting particles (ingesting cells). For the distinction between internalized and surface-bound FITC-labeled zymosan, trypan blue (1.2 mg/ml) was used to quench surface-bound fluorescence. Results: The technical challenges related to settings of phagocytotic gates and derivation of phagocytotic equa-

show abstract

Untitled

Flaminio

Rush

Davis

et al. 2002

Veterinary Research Communications

View full text Add to dashboard Cite

This paper describes a method for simultaneously measuring phagocytosis and oxidative burst activity in equine peripheral blood leukocytes by flow cytometry. Opsonized propidium iodide-labelled Staphylococcus aureus (PI-Sa) was used to measure the uptake of bacteria by equine phacocytes and the oxidative burst activity by oxidation of dihydrorhodamine 123. The requirements to achieve optimal activity of phagocytosis and oxidative burst are described. The advantage of the simultaneous technique is that it provides both independent and comparative values for phagocytosis and the oxidative burst, for the detection of impaired mechanisms of microbial destruction. Furthermore, the technique allows evaluation of opsonization activity in this context.

show abstract

Assessment of phagocytic activity in whole blood using laser flow cytometry

Cited by 4 publications

References 1 publication

Immunostimulatory Effects of the Anionic Alkali Mineral Complex BARODON on Equine Lymphocytes

Immunostimulatory Effects of the Anionic Alkali Mineral Complex BARODON on Equine Lymphocytes

Flow cytometric quantitative determination of ingestion by phagocytes needs the distinguishing of overlapping populations of binding and ingesting cells

Untitled

Contact Info

Product

Resources

About