Assessment of pesticides cytoxicity by means of bioelectric profiling of mammalian cells

Moschopoulou, Georgia; Dourou, Athanasia-Maria; Fidaki, Aggeliki; Kintzios, Spyridon

doi:10.1016/j.enmm.2017.10.004

Cited by 8 publications

(10 citation statements)

References 30 publications

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“…A narrower linear range of detection but greater sensitivity was determined for the amperometric approach, which required 15 min of exposure to cypermethrin. Quite recently, Moschopoulou et al [24] reported the application of a dual amperometric/potentiometric biosensor approach for the differential monitoring of the response of four different mammalian cell lines (two kidney, two neuroblastoma) against mixtures of pyrethroids, including cypermethrin. For this purpose, the authors applied a modification of the Bioelectric Recognition Assay (BERA) [20,25].…”

Section: Introductionmentioning

confidence: 99%

“…The BERA assay has been already been used for the detection of several pesticides (especially organophosphate and carbamates) in various food matrices [25][26][27]. In the study conducted by Moschopouolou et al [24], the authors were able to determine a unique pattern of response for each cell type, with amperometry being more reproducible than potentiometry. The pyrethroid mixture caused a concentration-dependent increase of bioelectric conductivity in neuroblastoma SK-N-SH cells, as wells as a considerable decrease in neuroblastoma N2a and kidney Vero cells.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of Cypermethrin Residues in Tobacco by a Bioelectric Recognition Assay (BERA) Neuroblastoma Cell-Based Biosensor

et al. 2019

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This study presents a bioelectric cell-based biosensor for the monitoring of the pyrethroid pesticide cypermethrin, a voltage-gated sodium channel blocker, in tobacco samples. For this purpose, neuroblastoma cells were used as biorecognition elements. The potential interference by the tobacco major alkaloid nicotine on the detection of cypermethrin was also studied. In addition, fluorescence microscopy revealed a specific pattern of neuroblastoma cell calcium efflux (Ca2+) after treatment with nicotine or cypermethrin. Finally, actual field-derived tobacco extracts were used for assessing matrix effects on the biosensor’s performance. The biosensor could detect cypermethrin in concentrations up to 1.5 μg mL−1 without being influenced by the presence of nicotine and possibly other tobacco alkaloids. Though not selective for cypermethrin, the neuroblastoma-based biosensor system appears to be a promising alternative to laborious analysis methodologies for rapid, high throughput and cost-efficient screening of this pyrethroid in tobacco samples in the near future.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Assessment of Cypermethrin Residues in Tobacco by a Bioelectric Recognition Assay (BERA) Neuroblastoma Cell-Based Biosensor

et al. 2019

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“…A customized biosensor, previously used specifically for BERA-based tests, was employed, allowing for either potentiometric or cyclic voltammetric measurements [17,18]. Using either approach, different concentrations of 2,4,6-TCA will produce a unique pattern of biosensor response, like a 'signature'.…”

Section: Biosensor Devicementioning

confidence: 99%

Comparative Study of a Cell-Based and Electrochemical Biosensor for the Rapid Detection of 2,4,6-Trichloroanisole in Barrel Water Extracts

Moschopoulou

Mavrikou

Valdés³

et al. 2018

Beverages

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(1) Background: Fungal metabolites such as haloanisoles (especially 2,4,6-tribromoanisole/ 2,4,6-TCA) are contaminants of cork and wood barrels, materials that are widely used in the wine industry. Thus, the accurate and timely detection of these substances is very important for this sector of beverage industry. (2) Methods: Potentiometry was used for the Bioelectric Recognition Assay (BERA)-based experimental approach, where changes in the electric properties of the Vero cells modified with anti-TCA antibodies were recorded in response to the presence of 2,4,6-TCA in different concentrations. Furthermore, a second electrochemical biosensor system based on the cyclic voltammetric (CV) measurement of a reaction taking place on a screen printed electrode was developed in parallel to the customized application and configuration of the cell-based system. (3) Results: The BERA cell-based biosensor was able to quantitatively differentiate among the lower 2,4,6-TCA concentrations (control, 0.25 and 1.25 ng/L) from spiked oak barrel water extracts in an entirely distinct and reproducible manner. In contrast, the CV method was not sensitive enough to differentiate between the samples. (4) Conclusions: The present study indicates that the BERA-based biosensor after further development and optimization could be used for the routine, high throughput detection of 2,4,6-TCA in oak barrel water extracts.

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“…The electrical properties of an organ can be used as a source of information about good health or bad an organ. Some studies show there is a difference between the electrical characteristics of normal and abnormal tissues [9,10].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Hair Spray Exposure to the Changes in Electrical Properties of Mice Organ

2020

IJITEE

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Human activities at this time tend to use substances that are pollutants and have an impact on health. Pollutants can enter the body through the process of respiration and will disrupt the organ function. Substances that are often used in daily life and potentially as pollutants are hairspray. They contain vinyl acetate and methacrylate complex polymers. Cocamide DEA surfactants in hairspray can cause cancer and produce toxins. The use of hairspray continuously will cause organ damage due to the emergence of secondary metabolites and free radicals. The diagnosis of organ damage in the latest research from various scientists is to utilize the electrical characteristics of the organ. The bioelectric characteristic that can be observed is dielectric constant. Based on the impact of organ damage and suspected changes in organ electrical properties due to exposure to pollutants, a study needs to be conducted to observe changes in the resistivity and dielectric constant in organ tissue in mice exposed to hairspray with cocamide DEA surfactant content. The results showed that the more concentration of exposure given causes increasing the level of damage to the organs. The increasing level of damage is very significant in the changing of the resistivity and dielectric constant value of mice organs. The changing of electrical properties of mice organs is possible due to damage to organ cells caused by increasing reactive oxygen species and free radicals due to oxidation reactions resulting from the interaction of cocamide DEA with cell components.

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Assessment of pesticides cytoxicity by means of bioelectric profiling of mammalian cells

Cited by 8 publications

References 30 publications

Assessment of Cypermethrin Residues in Tobacco by a Bioelectric Recognition Assay (BERA) Neuroblastoma Cell-Based Biosensor

Assessment of Cypermethrin Residues in Tobacco by a Bioelectric Recognition Assay (BERA) Neuroblastoma Cell-Based Biosensor

Comparative Study of a Cell-Based and Electrochemical Biosensor for the Rapid Detection of 2,4,6-Trichloroanisole in Barrel Water Extracts

The Effect of Hair Spray Exposure to the Changes in Electrical Properties of Mice Organ

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