Assessment of Pathogenic Potential, Virulent Genes Profile, and Antibiotic Susceptibility of <i>Proteus mirabilis</i> from Urinary Tract Infection

Hussein, Emad I.; Al‐Batayneh, Khalid; Masadeh, Majed M; Dahadhah, Fatina W.; Zoubi, Mazhar S. Al; Aljabali, Alaa A. A.; Alzoubi, Karem H.

doi:10.1155/2020/1231807

Cited by 21 publications

(23 citation statements)

References 19 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…hpmA , which is encoded by CriePir89, is mainly responsible for tissue damage [ 35 ]. Finally, rpoA gene associated with urovirulence and antibiotic efficiency [ 36 ] was revealed in this strain.…”

Section: Resultsmentioning

confidence: 99%

Multidrug-Resistant Proteus mirabilis Strain with Cointegrate Plasmid

et al. 2020

View full text Add to dashboard Cite

Proteus mirabilis is a component of the normal intestinal microflora of humans and animals, but can cause urinary tract infections and even sepsis in hospital settings. In recent years, the number of multidrug-resistant P. mirabilis isolates, including the ones producing extended-spectrum β-lactamases (ESBLs), is increasing worldwide. However, the number of investigations dedicated to this species, especially, whole-genome sequencing, is much lower in comparison to the members of the ESKAPE pathogens group. This study presents a detailed analysis of clinical multidrug-resistant ESBL-producing P. mirabilis isolate using short- and long-read whole-genome sequencing, which allowed us to reveal possible horizontal gene transfer between Klebsiella pneumoniae and P. mirabilis plasmids and to locate the CRISPR-Cas system in the genome together with its probable phage targets, as well as multiple virulence genes. We believe that the data presented will contribute to the understanding of antibiotic resistance acquisition and virulence mechanisms for this important pathogen.

show abstract

Section: Resultsmentioning

confidence: 99%

Multidrug-Resistant Proteus mirabilis Strain with Cointegrate Plasmid

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The rsb A gene expresses a membrane sensor that induces the production of extracellular polysaccharides. Also, it regulates the swarming phenomena and enhances the biofilm formation by P. mirabilis 23 , 50 , 52 , 53 .…”

Section: Discussionmentioning

confidence: 99%

atpD gene sequencing, multidrug resistance traits, virulence-determinants, and antimicrobial resistance genes of emerging XDR and MDR-Proteus mirabilis

Algammal

Hashem

Alfifi

et al. 2021

Sci Rep

View full text Add to dashboard Cite

Proteus mirabilis is a common opportunistic pathogen causing severe illness in humans and animals. To determine the prevalence, antibiogram, biofilm-formation, screening of virulence, and antimicrobial resistance genes in P. mirabilis isolates from ducks; 240 samples were obtained from apparently healthy and diseased ducks from private farms in Port-Said Province, Egypt. The collected samples were examined bacteriologically, and then the recovered isolates were tested for atpD gene sequencing, antimicrobial susceptibility, biofilm-formation, PCR detection of virulence, and antimicrobial resistance genes. The prevalence of P. mirabilis in the examined samples was 14.6% (35/240). The identification of the recovered isolates was confirmed by the atpD gene sequencing, where the tested isolates shared a common ancestor. Besides, 94.3% of P. mirabilis isolates were biofilm producers. The recovered isolates were resistant to penicillins, sulfonamides, β-Lactam-β-lactamase-inhibitor-combinations, tetracyclines, cephalosporins, macrolides, and quinolones. Using PCR, the retrieved strains harbored atpD, ureC, rsbA, and zapA virulence genes with a prevalence of 100%, 100%, 94.3%, and 91.4%, respectively. Moreover, 31.4% (11/35) of the recovered strains were XDR to 8 antimicrobial classes that harbored blaTEM, blaOXA-1, blaCTX-M, tetA, and sul1 genes. Besides, 22.8% (8/35) of the tested strains were MDR to 3 antimicrobial classes and possessed blaTEM, tetA, and sul1genes. Furthermore, 17.1% (6/35) of the tested strains were MDR to 7 antimicrobial classes and harbored blaTEM, blaOXA-1, blaCTX-M, tetA, and sul1 genes. Alarmingly, three strains were carbapenem-resistant that exhibited PDR to all the tested 10 antimicrobial classes and shared blaTEM, blaOXA-1, blaCTX-M, tetA, and sul1 genes. Of them, two strains harbored the blaNDM-1 gene, and one strain carried the blaKPC gene. In brief, to the best of our knowledge, this is the first study demonstrating the emergence of XDR and MDR-P.mirabilis in ducks. Norfloxacin exhibited promising antibacterial activity against the recovered XDR and MDR-P. mirabilis. The emergence of PDR, XDR, and MDR-strains constitutes a threat alarm that indicates the complicated treatment of the infections caused by these superbugs.

show abstract

“…Our results indicate a high prevalence of virulence factors among the studied isolates [ 23 ]. conducted a study on the virulence profile of P. mirabilis strains isolated from patients with UTI and found that 100% of the strains were positive for the mrpA , pmfA, and hmpA genes, whereas the zapA , ptA, and ucaA genes were found in 98.2%, 95.5%, and 95.5% of the strains, respectively [ 15 ]. also found high rates in their research: 98.4% of the strains were positive for the hpmA and atfA genes, and 92.1%, for the mrpA gene.…”

Section: Discussionmentioning

confidence: 99%

Virulence, resistance and clonality of Proteus mirabilis isolated from patients with community-acquired urinary tract infection (CA-UTI) in Brazil

Oliveira

Barbosa

Faustino

et al. 2021

Microbial Pathogenesis

View full text Add to dashboard Cite

Urinary tract infections (UTIs) are among the most common human infections, both in hospitals and in communities. Proteus mirabilis is known to cause community-acquired urinary tract infection (CA-UTI) and is an important causative agent of nosocomial UTIs. The pathogenesis of this species is related to its ability to manifest virulence factors, such as biofilms, adhesion molecules, urease, proteases, siderophores, and toxins. In this study, we investigated the virulence, sensitivity to antimicrobials, and clonal relationship of 183 strains isolated from the urine of CA-UTI patients in Londrina, Paraná State, Brazil. A total of 100% of the strains were positive for hpmA, ptA, zapA, mrpA, pmfA, ireA, and atfA virulence genes. The ucaA gene was positive in 81.4% of the cases. The strains showed high rates of sensitivity to the evaluated antimicrobials, and only one was ESBL-positive. All the tested bacteria showed the capacity to form biofilms: 73.2% had a very strong intensity, while 25.7% had a strong intensity, and 1.1% had a moderate intensity. Regarding clonality, 40 clonal clusters were found among the microorganisms tested. Our results showed that strains of P. mirabilis isolated from CA-UTI patients have several virulence factors. Although the urinary clinical isolates studied showed high sensitivity to antimicrobials, the strains showed a strong capacity to form biofilms, making antibiotic therapy difficult. In addition, it was observed that there were clones of P. mirabilis circulating in the city of Londrina.

show abstract

Assessment of Pathogenic Potential, Virulent Genes Profile, and Antibiotic Susceptibility of Proteus mirabilis from Urinary Tract Infection

Cited by 21 publications

References 19 publications

Multidrug-Resistant Proteus mirabilis Strain with Cointegrate Plasmid

Multidrug-Resistant Proteus mirabilis Strain with Cointegrate Plasmid

atpD gene sequencing, multidrug resistance traits, virulence-determinants, and antimicrobial resistance genes of emerging XDR and MDR-Proteus mirabilis

Virulence, resistance and clonality of Proteus mirabilis isolated from patients with community-acquired urinary tract infection (CA-UTI) in Brazil

Contact Info

Product

Resources

About