Assessment of paper spray ionization for quantitation of pharmaceuticals in blood spots

Manicke, Nicholas E.; Yang, Qian; Wang, He; Oradu, Sheran; Ouyang, Zheng; Cooks, R. Graham

doi:10.1016/j.ijms.2010.06.037

Cited by 170 publications

(187 citation statements)

References 23 publications

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“…Although not yet applied to lipids, PSI has also shown quantitative capabilities by doping the paper or sample with an internal standard [275]; such approaches may allow the rapid quantitation of lipid extracts and possible even tissues. Furthermore, it is possible that liquid extraction and spray-based approaches will find success for relative quantitation due to the ease in which internal standards may be added to the extraction or spray solvent [276], although to our knowledge this has yet to be reported for lipid analysis.…”

Section: Discussionmentioning

confidence: 99%

Surface analysis of lipids by mass spectrometry: More than just imaging

Ellis

Brown

Panhuis

et al. 2013

Progress in Lipid Research

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Mass spectrometry is now an indispensable tool for lipid analysis and is arguably the driving force in the renaissance of lipid research. In its various forms, mass spectrometry is uniquely capable of resolving the extensive compositional and structural diversity of lipids in biological systems. Furthermore, it provides the ability to accurately quantify molecular-level changes in lipid populations associated with changes in metabolism and environment; bringing lipid science to the "omics" age. The recent explosion of mass spectrometry-based surface analysis techniques is fuelling further expansion of the lipidomics field. This is evidenced by the numerous papers published on the subject of mass spectrometric imaging of lipids in recent years. While imaging mass spectrometry provides new and exciting possibilities, it is but one of the many opportunities direct surface analysis offers the lipid researcher. In this review we describe the current state-ofthe-art in the direct surface analysis of lipids with a focus on tissue sections, intact cells and thin-layer chromatography substrates. The suitability of these different approaches towards analysis of the major lipid classes along with their current and potential applications in the field of lipid analysis are evaluated. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 2 AbstractMass spectrometry is now an indispensable tool for lipid analysis and is arguably the driving force in the renaissance of lipid research. In its various forms, mass spectrometry is uniquely capable of resolving the extensive compositional and structural diversity of lipids in biological systems. Furthermore, it provides the ability to accurately quantify molecular-level changes in lipid populations associated with changes in metabolism and environment;bringing lipid science to the "omics" age. The recent explosion of mass spectrometry-based surface analysis techniques is fuelling further expansion of the lipidomics field. This is evidenced by the numerous papers published on the subject of mass spectrometric imaging of lipids in recent years. While imaging mass spectrometry provides new and exciting possibilities, it is but one of the many opportunities direct surface analysis offers the lipid researcher. In this review we describe the current state-of-the-art in the direct surface analysis of lipids with a focus on tissue sections, intact cells and thin-layer chromatography substrates.The suitability of these different approaches towards analysis of the major lipid classes along with their current and potential applications in the field of lip...

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Section: Discussionmentioning

confidence: 99%

Surface analysis of lipids by mass spectrometry: More than just imaging

Ellis

Brown

Panhuis

et al. 2013

Progress in Lipid Research

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“…Some noncapillary tips such as stainless steel needle [6,19], paper [13,[20][21][22][23], and wooden tips [14] enabled direct analysis of raw samples. Hiraoka and co-workers reported the sequential and exhaustive ionization of analytes on solid metal probe [24].…”

Section: E Lectrospray Ionization (Esi) Is a Powerful Massmentioning

confidence: 99%

Analytical Properties of Solid-substrate Electrospray Ionization Mass Spectrometry

Yao

2012

J. Am. Soc. Mass Spectrom.

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Abstract. Conventional electrospray ionization mass spectrometry (ESI-MS) uses a capillary for sample loading and ionization. Along with the development of ambient ionization techniques, ESI-MS using noncapillary emitters has attracted more interest in recent years. Following our recent report on ESI-MS using wooden tips (Anal. Chem. 83, 8201-8207 (2011)), the technique was further investigated and extended in this study. Our results revealed that the wooden tips could serve as a chromatographic column for separation of sample components. Sequential and exhaustive ionization was observed for proteins and salts on wooden tips with salts ionized sooner and proteins later. Nonconductive materials that contain microchannels/pores could be used as tips for ESI-MS analysis with sample solutions loaded to the sharp-ends only, since rapid diffusion of sample solutions by capillary action would enable the tips to become conductive. Tips of inert materials such as bamboo, fabrics, and sponge could be used for sample loading and ionization, while samples such as tissue, mushroom, and bone could form tips to induce ionization for direct analysis with application of a high voltage.

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“…As the solvent wicks through the sample, soluble components are extracted into the solvent, leaving behind the bulk of proteins and lipids [6]. A high voltage (3-5 kV) is applied to the moist paper, and an electrospray is induced at the sharp tip of the triangular shaped paper in the cartridge, which is held stationary in front of the inlet to the MS. As solvent evaporates, gas phase ions of the analyte molecules are generated which can then be detected by MS. PS-MS/MS has been successfully used for quantitative analysis of xenobiotics (8)(9)(10)) and endogenous compounds (11) from whole blood.…”

Section: Introductionmentioning

confidence: 99%