Assessment of Optimized Frozen/Thawed Semen Samples in Canines with the New A-Kinase Anchor Protein 4 Precursor Biomarker

Langlade, Camille; Buff, Samuel; Dias, Catia; Commin, Loris

doi:10.1089/bio.2020.0019

Cited by 4 publications

(3 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ProAKAP4 and its active form, AKAP4, are expressed in a variety of mammalian species, and they are highly conserved (~70%) in the animal kingdom [ 9 ]. They are present in the horse [ 13 ], bull [ 14 ], pig [ 15 ], ram [ 16 ], koala [ 17 ], crocodile [ 18 ], camel [ 19 ] and dog [ 20 , 21 , 22 ]. ProAKAP4 and AKAP4 constitute a unique fibrillar structure never observed in other flagellated cell types [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

ProAKAP4 Concentration Is Related to Sperm Motility and Motile Sperm Subpopulations in Frozen–Thawed Horse Semen

Dordas-Perpinyà

Yánez-Ortiz

Sergeant

et al. 2022

Animals

View full text Add to dashboard Cite

ProAKAP4 is the precursor of AKAP4 (A-kinase Anchor protein 4), the main structural protein of the fibrous sheath of sperm. The amount of proAKAP4 reflects the ability of spermatozoa to maintain the flagellum activity and functionality up to the site of fertilization and is positively correlated with progressive motility in several mammalian species. The aim of this study was to investigate the relationship between proAKAP4 concentration with horse sperm motility descriptors and spermatic motile subpopulations. For this purpose, a total of 48 ejaculates from 13 different stallions were analyzed. Spermatic motility descriptors were obtained by the CASA system, and four motile subpopulations (SP) with specific motility patterns were statistically identified. ProAKAP4 concentrations were evaluated by ELISA. The relationship between motility descriptors of sperm subpopulations and proAKAP4 concentrations was evaluated. Following a hierarchical cluster statistical analysis, ejaculates were divided into two groups according to their proAKAP4 concentrations, either having low proAKAP4 concentrations (5.06–35.61 ng/10M spz; n = 23) or high (39.92–82.23 ng/10M spz; n = 25) proAKAP4 concentrations (p < 0.001). ProAKAP4 concentrations were positively correlated (p < 0.05) with total and progressive motility, as well as with parameters of velocity. ProAKAP4 amount also showed a negative correlation (p < 0.05) with sperm motile subpopulation number 3, which was the subpopulation with the lowest velocity parameters. In conclusion, proAKAP4 concentration in stallion semen positively reflects sperm progressive motility with the functional velocity kinematic descriptors. Concentrations of proAKAP4 higher than 37.77 ng/10M spz were correlated with a very good quality frozen/thawed stallion semen.

show abstract

Section: Introductionmentioning

confidence: 99%

ProAKAP4 Concentration Is Related to Sperm Motility and Motile Sperm Subpopulations in Frozen–Thawed Horse Semen

Dordas-Perpinyà

Yánez-Ortiz

Sergeant

et al. 2022

Animals

View full text Add to dashboard Cite

show abstract

“…In canine frozen/thawed semen, the expression of the precursor was found to be highly variable and dependent on incubation time and straw size; a correlation between proAKAP4 levels and motility or other velocity parameters could not be demonstrated [78,86]. However, it was suggested that the degree of proAKAP4 expression is related to the recovery and maintenance of velocity [78]. The latter might be interesting for the prediction of the fertilizing capability of freeze-thawed samples; however, this hypothesis has to be proven using further analyses.…”

Section: Membrane Proteinsmentioning

confidence: 92%

“…In pigs, the stability of proAKAP4 after thawing proved to be a quality marker [85]. In canine frozen/thawed semen, the expression of the precursor was found to be highly variable and dependent on incubation time and straw size; a correlation between proAKAP4 levels and motility or other velocity parameters could not be demonstrated [78,86]. However, it was suggested that the degree of proAKAP4 expression is related to the recovery and maintenance of velocity [78].…”

Section: Membrane Proteinsmentioning

confidence: 99%

Canine Spermatozoa—Predictability of Cryotolerance

Schäfer-Somi

Colombo

Luvoni

2022

Animals

View full text Add to dashboard Cite

Markers of freezability allow the selection of ejaculates of good freezability. So far, most investigations were conducted in boars, bulls, rams and horses, with high economic interests triggering the efforts. The progress in dogs is comparably slow. A critical evaluation of the methods requires consideration of practicability, with most labs not even possessing a computer assisted sperm analyser (CASA); furthermore, small canine ejaculates mostly do not allow the use of large semen volumes. In dogs, modern markers of freezability no longer assess single membrane constituents or seminal plasma components but comprise tests of cell functionality and adaptability, energy metabolism, cluster analyses of kinetic and morphometric parameters, as well as DNA intactness. Identification of the most efficient combination of tests seems useful. At present, examination by CASA combined with cluster analysis of kinetic subgroups, JC-1 staining and COMET assay or staining with toluidine blue seem most appropriate; however, cell volumetry and other functional tests deserve better attention. A better understanding of spermatozoa energy metabolism might reveal new markers. This review focuses on the requirements and markers of freezability of canine semen, highlighting potential future candidates.

show abstract