Assessment of oocyte source for ovine nuclear transfer

McLaughlin, K. John; Pugh, A.; Logan, Kay; Tervit, H.R.

doi:10.1016/0093-691x(91)90216-z

Cited by 2 publications

(2 citation statements)

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“…Furthermore, the morphological quality of in vivo cultured morulae and blastocysts derived from in vitro and in vivo matured oocytes were similar. Hence, these results confirm earlier indications (McLaughlin et al 1991;Pugh et al 1992) that ovine in vitro matured oocytes can replace in vivo matured oocytes, as has recently been shown in cattlecloning programmes (Barnes et al 1993;Yang et al 1993;Heyman et al 1994). Two differences between in vivo and in vitro methods were observed:…”

Section: Discussionsupporting

confidence: 91%

“…The overall fusion rates in this study were similar to one report dealing with in vitro matured ovine oocytes (Pugh et al 1992), but lower than others have reported for ovine in vivo oocytes (Willadsen 1986;Wilmut and Smith 1989;McLaughlin et al 1990McLaughlin et al , 1991. The necessity of fine-tuning the electrofusion parameters for the specific system in order to obtain optimal results is thus highlighted.…”

Section: Discussionsupporting

confidence: 82%

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In vitro and in vivo Development of Cloned Ovine Embryos using in vitro and in vivo Matured Oocytes

Holm

Nagashima

Sun

et al. 1995

Reprod Domestic Animals

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ContentsCloning of sheep embryos by nucleus transplantation can be achieved by using in vivo matured (oviductal) oocytes and in vivo culture. However, these steps involve cumbersome procedures. Therefore, the effects of in vivo vs. the equivalent in vitro procedures on the pre-implantation development of cloned embryos were compared using: 1. In vivo oocytes and in vivo culture; 2. In vivo oocytes and in vitro culture; and 3. In vitro oocytes and in vitro culture. Selected embryos were transferred to recipients.Donor embryos and oviductal oocytes were collected from superovulated Merino ewes. In vivo matured oocytes were enucleated and fused with inserted blastomeres from donor embryos. In vitro matured oocytes were enucleated and allowed to age prior to blastomere insertion and electrofusion. Fused embryos were cultured for approximately 132 h either in vivo in ligated sheep oviducts or in vitro, and those developed beyond the eight cell stage were transferred to recipient ewes.More in vifro than in vivo matured oocytes fused (66 vs. 43%, p

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