Assessment of nitrogen fixation inPterocarpus erinaceusandP. lucensusing15N labeling techniques

Sylla, Samba Ndao; Ndoye, Ibrahima; Bâ, Amadou; Gueye, Mamadou; Dreyfus, B

doi:10.1080/15324989809381513

Cited by 7 publications

(3 citation statements)

References 18 publications

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“…Our results confirmed those of Gueye et al (1997) who observed that some seed sources of Faidherbia albida produced plants of similar dry weight, but different nitrogen fixing capacity. Our results also confirmed those of earlier studies showing that leaves can be indicative of nitrogen fixation by the whole plant (Danso et al 1995;Sylla et al 1998;Raddad et al 2005). Some studies, however, demonstrated that proportions of fixed nitrogen differed by plant part (Butler1987; Ladd 1981).…”

Section: Discussionsupporting

confidence: 95%

Symbiotic nitrogen fixation in black locust (Robinia pseudoacacia L.) seedlings from four seed sources

Moshki

Lamersdorf

2011

Journal of Forestry Research

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We conducted a greenhouse experiment to investigate the role of seed source in growth and symbiotic nitrogen fixation of black locust (Robinia pseudoacacia L). Seeds from different sources were planted in the same environmental conditions and inoculated with a suspension of mixed Rhizobium. We used the modified 15 N isotope dilution method to estimate biological nitrogen fixation of Robinia trees. Different Robinia seed sources differed significantly in terms of tissue dry weight (50.6−80.1 g), total N (1.31-2.16 g) and proportion of nitrogen derived from the atmosphere ( 0-51%). A higher nitrogen fixation rate of Robinia trees was associated with higher dry weight. Moreover, the leaves of Robinia proved to adequately represent the nitrogen fixation capacity of entire plants. Our results confirmed that assessment of seed sources is a useful way to improve the nitrogen fixation capacity and therefore the growth rate of Robinia.

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Section: Discussionsupporting

confidence: 95%

Symbiotic nitrogen fixation in black locust (Robinia pseudoacacia L.) seedlings from four seed sources

Moshki

Lamersdorf

2011

Journal of Forestry Research

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“…Ndoye et al (1995) studying different acacias including A. senegal using the 15 N isotope dilution technique found that leaves and stems had similar 15 N enrichments. Similarly, estimates of %N dfa do not differ significantly between different plant parts or between whole trees grown in greenhouse or in the field Gueye et al, 1997;Sylla et al, 1998). However, some authors reported differences in 15 N between different plant parts (Boddey et al, 2000).…”

Section: Discussionmentioning

confidence: 88%

Symbiotic nitrogen fixation in eight Acacia senegal provenances in dryland clays of the Blue Nile Sudan estimated by the 15N natural abundance method

et al. 2005

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The symbiotic biological N 2 fixation by Acacia senegal was estimated using the 15 N natural abundance (d 15 N) procedure on eight provenances collected from different environments and soil types grown in a clay soil in the Blue Nile region, Sudan. Balanites aegyptiaca (a non-legume) was used as a non-N 2 -fixing reference plant to allow 15 N-based estimates of the proportion of the Acacia N derived from atmospheric N 2 (N dfa ) to be calculated. Results show variation in leaf d 15 N between A. senegal and the reference plant and among years. The relative d 15 N values (&) were higher in B. aegyptiaca than in the N 2 -fixing acacia provenances. Provenances originally collected from clay soils fixed little N in the first year, but the amount fixed increased as the trees aged. All provenances showed a decrease in d 15 N with age. The N dfa varied between 24% (Mazmoom provenance) and 61% (Rahad provenance) 4 years after planting. There was no significant difference in d 15 N between provenance groups based on soil type or rainfall at original growing site. The amount of N dfa increased significantly with age in all provenances. The above-ground contribution of fixed N to foliage growth in a 4-year-old A. senegal was highest in the Rahad sand-soil provenance (46.7 kg N ha )1 ) and lowest in the Mazmoom clay-soil provenance (28.7 kg N ha )1 ). Our study represents the first use of the d 15 N method for estimating the N input by A. senegal to the clay plain soils of the gum belt in the Sudan.

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“…In contrast, ignoring roots may result in significant underestimation of nitrogen fixed in many nitrogen-fixing trees as have been reported in Leucaena leucocephala and Gliricidia sepium (Sanginga For each plant part, values in the same column followed by the same letter do not differ significantly at P= 0.05. CV = coefficient of variation et al, 1992), Acacia spp (Ndoye et al, 1995), Faidherbia albida (Gueye et al, 1997) and Pterocarpus spp (Sylla et al, 1999). These experiments were carried out in greenhouse conditions, but further studies are planned to estimate the actual nitrogen fixation of the Crotalaria species in the field.…”

Section: Discussionmentioning

confidence: 99%

Biological nitrogen fixation in Crotalaria species estimated using the 15N isotope dilution method

Samba¹,

Sylla²,

Neyra³

et al. 2002

Afr. J. Biotechnol.

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A greenhouse experiment was conducted to measure nitrogen fixation in three Crotalaria species : C. ochroleuca, C. perrottetii and C. retusa growing in Senegal by using 15 N direct isotope dilution technique. Two non-fixing plants, Senna obtusifolia and Senna occidentalis served as reference plants. The amount of nitrogen fixed two months after planting was obtained using the average of the two reference plants. The atom % 15 N excess in the Crotalaria species was significantly lower than that of the reference plants, indicating that significant nitrogen fixation occurred in the three plants. Significant differences were observed between the Crotalaria species; C. ochroleuca yielded more dry matter weight and total nitrogen than did C. perrottetti and C. retusa. The % nitrogen derived from atmosphere (%Ndfa) in leaves and stems was also higher in C. ochroleuca. There was no significant difference in %Ndfa in the whole plant between the three Crotalaria species (47% to 53%). In contrast, interspecific variability was observed based on the %Ndfa. C. ochroleuca significantly exhibited the higher amount of total nitrogen fixed, equivalent to 83 kg of nitrogen fixed per hectare. Based on these data, it was concluded that C. ochroleuca could be used in multiple cropping systems in Senegal for making more nitrogen available to other plants.

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Assessment of nitrogen fixation inPterocarpus erinaceusandP. lucensusing¹⁵N labeling techniques

Cited by 7 publications

References 18 publications

Symbiotic nitrogen fixation in black locust (Robinia pseudoacacia L.) seedlings from four seed sources

Symbiotic nitrogen fixation in black locust (Robinia pseudoacacia L.) seedlings from four seed sources

Symbiotic nitrogen fixation in eight Acacia senegal provenances in dryland clays of the Blue Nile Sudan estimated by the 15N natural abundance method

Biological nitrogen fixation in Crotalaria species estimated using the 15N isotope dilution method

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