Assessment of neutralizing antibodies elicited by a vaccine 
(Nakayama) strain of Japanese encephalitis virus in Taiwan

Shyu, W. R.-H.; Wang, Y.-C.; Chin, Chuan; Chen, W.-J.

doi:10.1017/s095026889700753x

Cited by 24 publications

(16 citation statements)

References 22 publications

(25 reference statements)

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“…This result coincides with the findings of a 1991 JE seroepidemiologic study in Taiwan. 40 Similar shifting of onset age was also observed in Korea and China after initiation of JE vaccination programs. 41,42 Vaccine efficacy after more than 2 doses of vaccine is estimated to be about 85%.…”

Section: Discussionsupporting

confidence: 60%

The epidemiology of Japanese encephalitis on Taiwan during 1966-1997.

Huang²,

Chien³

et al. 1999

The American Journal of Tropical Medicine and Hygiene

103

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Abstract. Japanese encephalitis (JE) is an endemic disease in Taiwan. A mass vaccination program of children against JE was first implemented in 1968. Along with general improvements in various aspects of living conditions over the years, the program has brought JE well under control. The main characteristics of JE epidemiology in Taiwan in the past 3 decades are as follows. The transmission mode remains unchanged-that is, the amplification stage of the virus in pigs is followed by a human epidemic each year. The frequency of JE incidence has dropped significantly.

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Section: Discussionsupporting

confidence: 60%

The epidemiology of Japanese encephalitis on Taiwan during 1966-1997.

Huang²,

Chien³

et al. 1999

The American Journal of Tropical Medicine and Hygiene

103

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“…An inactivated vaccine derived from Nakayama-NIH strain JE virus-infected mouse brain has remained dominant in the Taiwanese market up to now [1]. However, those who possess neutralizing antibodies induced by the Nakayama vaccine may not be efficiently protected from the infection by the wild strain virus [2].…”

Section: Introductionmentioning

confidence: 99%

Acute Flaccid Paralysis as an Unusual Presenting Symptom of Japanese Encephalitis: A Case Report and Review of the Literature

et al. 2007

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Japanese encephalitis (JE) is an endemic disease in Taiwan. Acute JE virus infection characterized by acute flaccid paralysis in an adult has never been reported in Taiwan. We report a young adult man who received four doses of JEV (Nakayama strain) vaccination in childhood, but still developed acute JE virus infection, characterized with acute flaccid paralysis.He presented with fever, headache, progressive muscle weakness, and respiratory paralysis requiring mechanical ventilator. Deep tendon reflexes were decreased except for the Achilles reflex. After supportive care, he was weaned from the mechanical ventilator and at discharge 1 month later, his muscle power level and deep tendon reflexes recovered partially. The diagnosis of JE was based on the presence of anti-JE virus IgM in the CSF and seroconversion of IgM and IgG by the ELISA method. Electrophysiological findings were described. From the experience of this case, we caution that a history of vaccination for JE with the Nakayama strain may not provide a complete protection against natural infection in the community; and in Taiwan or any area where JE remains an endemic disease, Japanese virus encephalitis infection should be considered as a differential diagnosis in any adult presenting with acute flaccid paralysis.

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“…It has been reported that the current JEV vaccines do not afford complete protection against challenge with heterologous strains of JEV [26,27] due to the antigenic differences between JEV strains of a wide range of genotypes. In this study, when Balb/c mice immunized with pJBE and inactivated vaccine were challenged with different strains of JEV, protective immunity induced by inactivated vaccine (Beijing-1 strain of JEV) could not provide strong protection against lethal heterologous JEV challenge.…”

Section: Discussionmentioning

confidence: 99%

Immunologic Analysis Induced by DNA Vaccine Encoding E Protein of Beijing-1 Strain Derived from Japanese Encephalitis Virus

Gao¹,

Zhou²,

Zhai³

et al. 2006

Intervirology

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Objective: We have compared the gene expression and DNA immunization efficacy encoding prME and E proteins of a different strain (JaGAr-01) derived from Japanese encephalitis virus. This study aimed to construct a recombinant encoding E protein of the Beijing-1 strain derived from Japanese encephalitis virus and analyze the humoral, cellular and protective immunity induced by the above recombinant. Methods: The recombinant pJBE containing E (1,500 bps) gene from the Beijing-1 strain of Japanese encephalitis virus was constructed and then transfected into the HepG2 cell line by liposome fusion. The expression of E (about 53 kD) protein in transfected cells was analyzed by Western blot using a specific anti-JEV-E antibody. BALB/c mice were vaccinated with 3 µg of pJBE by the gene-gun technique. JaGAr-01 and Beijing-1 strains (10⁵ PFU/100 µl) of Japanese encephalitis virus were given to BALB/c mice by intraperitoneal injection 3 weeks after double DNA immunization with a lethal virus challenge. BALB/c mice were observed for 21 days after challenge. An 80% plaque reduction neutralization test was performed to titrate the neutralization antibody before and after viral challenge. A lactate dehydrogenase activity release test was used to examine cytotoxic T lymphocyte activity after double DNA immunization. Results: The expression of about 53 kD protein associated with pJBE was determined in transfected HepG2 cells with specific anti-JEV-E antibody. A higher level of neutralization antibodies and the cytotoxicity effect were induced with pJBE immunization using the gene-gun technique, and were similar to those induced with inactivated vaccine derive from the Beijing-1 strain of Japanese encephalitis virus. Balb/c mice immunized with pJBE survived the challenge with the different strains of Japanese encephalitis virus; however, Balb/c mice immunized with inactivated vaccine did not survive the challenge with the JaGAr-01 strain of Japanese encephalitis virus at all. Conclusions: DNA vaccine containing the E protein gene derived from Japanese encephalitis virus can provide not only better efficacy including humoral and cellular immunity, but also cross-protection against infection with homologous and heterologous Japanese encephalitis virus.

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Assessment of neutralizing antibodies elicited by a vaccine (Nakayama) strain of Japanese encephalitis virus in Taiwan

Cited by 24 publications

References 22 publications

The epidemiology of Japanese encephalitis on Taiwan during 1966-1997.

The epidemiology of Japanese encephalitis on Taiwan during 1966-1997.

Acute Flaccid Paralysis as an Unusual Presenting Symptom of Japanese Encephalitis: A Case Report and Review of the Literature

Immunologic Analysis Induced by DNA Vaccine Encoding E Protein of Beijing-1 Strain Derived from Japanese Encephalitis Virus

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