Assessment of Neuroprotective Effects of Neurotropin in Cultured Rat Dorsal Root Ganglion Neurons Using High- Throughput Imaging

Abstract

Background: Neurotropin, a non-protein extract derived from inflamed rabbit skin following inoculation with vaccinia virus, is an analgesic agent. The mechanism of action of neurotropin has been partially clarified. No experimental study, however, has been undertaken to assess oxaliplatin-induced neurotoxicity using quantitative in vitro high-throughput image analysis. In the present study, to elucidate the action of neurotropin on dorsal root ganglion (DRG) neurons, we explored the antioxidative and anti-inflammatory activity of neurotropin. We assessed the viability and morphological changes in cultured rat DRG neurons treated with oxaliplatin and neurotropin.

Neurotropin protects rotator cuff tendon cells from lidocaine-induced cell death

“…Neurotropin (NTP) is a drug derived from the non-protein fraction extracted from the inflamed skin of rabbits after vaccinia virus administration [1,[16][17][18][19][20][21][22]. NTP has been safely used in Japan for more than 50 years to treat various chronic pain conditions, such as lower back pain, cervico-omo-brachial syndrome, postherpetic neuralgia, hyperesthesia of subacute myelo-optic neuropathy, and other painful conditions [23].…”

“…NTP significantly reduced bidirectional axonal transport in time- and concentration-dependent manners without affecting the neurite diameter [ 16 ]. NTP alleviated oxaliplatin-induced apoptosis via the apoptosis signal regulating kinase 1 (ASK1)-p38 signaling pathway in dorsal root ganglion neurons by inducing thioredoxin [ 19 ]. A T-2 mapping study demonstrated that NTP administration improved knee pain in osteoarthritis without affecting the intra-articular proteoglycan concentration [ 18 ].…”