Assessment of Mycobacterial, Propionibacterial, and Human Herpesvirus 8 DNA in Tissues of Greek Patients with Sarcoidosis

Gazouli, Maria; Ikonomopoulos, John; Trigidou, Rodoula; Foteinou, Marianthi; Kittas, Christos; Gorgoulis, Vassilis G.

doi:10.1128/jcm.40.8.3060-3063.2002

Cited by 66 publications

(48 citation statements)

References 30 publications

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“…The oldest hypothesis of mycobacterial association remains controversial but persists (M. tuberculosis complex and mycobacteria other than M. tuberculosis), but recent studies have found that propionibacterial DNA appears to be more prevalent than mycobacterial DNA in lymph nodes from patients with sarcoidosis in Japan and some European countries [12, 13]. However, these results differ from a series of Greek patients with sarcoidosis, in which propionibacterium-positive sarcoidosis patients were found to be a subgroup (43%) of the mycobacterial-positive sarcoidosis subjects (71%) [14]. In general, the results of studies using PCR methodologies vary greatly, with mycobacterial nucleic components being detected in frequencies ranging from 0 to 70% of affected sarcoidosis tissues (table 3) [12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32].…”

Section: Discussioncontrasting

confidence: 54%

High Prevalence of <i>Mycobacterium tuberculosis</i> DNA in Biopsies from Sarcoidosis Patients from Catalonia, Spain

Fité

Fernández‐Figueras²,

Prats³

et al. 2006

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Background: Sarcoidosis is a systemic granulomatous disease of unknown etiology. The presence of mycobacterial nucleic acid components in patients with sarcoidosis has been demonstrated with varying degrees of success. Objectives: The aim of this study was to estimate the presence of Mycobacterium tuberculosis DNA in tissues from sarcoidosis patients, in Catalonia, Spain, as well as to assess the long-term clinical course in these patients. Methods: Fifty-eight paraffin-embedded tissue biopsies corresponding to cases of sarcoidosis (n = 23), lung neoplasm (n = 23), and lung tuberculosis (n = 12) available in 1996 were analyzed in a retrospective study by means of a nested polymerase chain reaction using primers corresponding to the insertion element IS6110 of M. tuberculosis complex. For greater sensitivity, Southern blot hybridization was performed. Clinical data were recorded prior to and after PCR analysis (follow-up reported until 2002). Results:M. tuberculosis DNA was present in 9 out of 23 sarcoidosis biopsies (39%), in 1 out of 23 control patients (4%) (p < 0.01), and in all tissue samples from the 12 control patients with tuberculosis. To date, none of these sarcoidosis patients has developed tuberculosis over a mean (±SD) follow-up period of 11 (±3.4) years. Conclusion: In our setting, M. tuberculosis DNA is present in tissue biopsies of significantly more sarcoidosis patients than controls. However, these results do not demonstrate causality, although they may suggest a link between M. tuberculosis infection and sarcoidosis in some cases. Follow-up of these patients suggests that M. tuberculosis-DNA-positive sarcoidosis patients are not at greater risk of developing tuberculosis than M. tuberculosis-DNA-negative patients.

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Section: Discussioncontrasting

confidence: 54%

High Prevalence of <i>Mycobacterium tuberculosis</i> DNA in Biopsies from Sarcoidosis Patients from Catalonia, Spain

Fité

Fernández‐Figueras²,

Prats³

et al. 2006

Respiration

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“…In addition, formalin-fixed paraffin-embedded material (lung tissue) from 32 Greek patients with tuberculosis (T1 to T32) was analyzed in parallel as controls. DNA extraction was performed as previously described (4). To confirm the integrity of the DNAs, a 430-bp sequence of the human glyceraldehyde-3-phosphate dehydrogenase gene was amplified.…”

mentioning

confidence: 99%

Characterization of Mycobacterium tuberculosis Complex Isolates from Greek Patients with Sarcoidosis by Spoligotyping

et al. 2005

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Spoligotyping was undertaken with 38 Mycobacterium tuberculosis isolates from Greek sarcoidosis patients and 31 isolates from patients with tuberculosis. Fifty percent of the isolates from sarcoidosis patients and 16.13% of the isolates from patients with tuberculosis were represented by a unique pattern, whereas the remaining isolates belonged to seven shared types. Interestingly, half of the isolates from sarcoidosis patients did not resemble the spoligotypes of the isolates from patients with tuberculosis, most of which pertained to shared spoligotypes.

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“…Several lines of evidence support the idea that sarcoidosis results from exposures to possible environmental agents such as Mycobacterium tuberculosis (M. tuberculosis) and Propionibacterium acnes (P. acnes) [87][88][89]. M. tuberculosis or P. acnes associated with anti-TNF treatment was also reported [90,91].…”

Section: Anti-tnf-associated Sarcoidosismentioning

confidence: 93%

The Role of Type I IFN and TNF-α in the Pathogenesis of Sarcoidosis

Akahoshi¹

2013

Sarcoidosis

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Assessment of Mycobacterial, Propionibacterial, and Human Herpesvirus 8 DNA in Tissues of Greek Patients with Sarcoidosis

Cited by 66 publications

References 30 publications

High Prevalence of <i>Mycobacterium tuberculosis</i> DNA in Biopsies from Sarcoidosis Patients from Catalonia, Spain

High Prevalence of <i>Mycobacterium tuberculosis</i> DNA in Biopsies from Sarcoidosis Patients from Catalonia, Spain

Characterization of Mycobacterium tuberculosis Complex Isolates from Greek Patients with Sarcoidosis by Spoligotyping

The Role of Type I IFN and TNF-α in the Pathogenesis of Sarcoidosis

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