Assessment of mutagenic sensitivity in blackgram variety CO 6

Abstract

The present study was conducted to examine the mutagenic effect of 60 Co gamma rays and a combination of 60 Co gamma rays with EMS in blackgram variety CO 6. Based on the LD 50 dose, the seeds were treated with three different doses of gamma rays (200, 300, 400 Gy) and recurrent doses of 20 mM and 30 mM EMS were treated with gamma ray irradiated seeds for combination treatments. The M 1 generation was evaluated in the field for the following parameters viz., germination percentage, plant survival percentage, plant height at 30 DAS and maturity, pollen fertility and seed fertility. A dose-dependent decrease was noticed for all the characters under this study in M 1 generation. Overall, the maximum reduction was noticed at a higher dose of 400 Gy gamma rays and 400 Gy + 30 mM EMS mutagenic treatments. Among the mutagenic treatments, a combination of 60 Co gamma ray + EMS exhibited the maximum effect with a higher percentage of reduction in all the traits while increased pollen sterility was found to be associated with a corresponding increase in dose/concentration of mutagenic agents. A significant positive correlation was observed between viable mutation frequency and biological damage caused by mutagens in M 1 generation. Hence, the higher doses of 60 Co gamma rays (300 and 400 Gy) and 60 Co gamma rays +EMS (300 Gy+ 30; 400 Gy+ 20 and 400 Gy+ 30 mM) proved to be efficient in increasing the mutation frequency towards desirable directions.

Mutagenic effectiveness, efficiency and dose optimization of gamma rays in papaya (Carica papaya L.) varieties

“…This is in close agreement with the reports of Dutta et al (2021) in bitter gourd. Tamilzharasi et al (2021) reported that there was a substantial positive correlation between mutation frequency and biological damage in the M1 generation when exposed to gamma radiation in blackgram. Mutagenic effectiveness refers to the rate at which a mutation is induced as a function of the mutagenic dose.…”