Assessment of Multi Fragment Melting Analysis System (MFMAS) for the Identification of Food-Borne Yeasts

Abstract

Multi Fragment Melting Analysis System (MFMAS) is a novel approach that was developed for the species-level identification of microorganisms. It is a software-assisted system that performs concurrent melting analysis of 8 different DNA fragments to obtain a fingerprint of each strain analyzed. The identification is performed according to the comparison of these fingerprints with the fingerprints of known yeast species recorded in a database to obtain the best possible match. In this study, applicability of the yeast version of the MFMAS (MFMAS-yeast) was evaluated for the identification of food-associated yeast species. For this purpose, in this study, a total of 145 yeast strains originated from foods and beverages and 19 standard yeast strains were tested. The DNAs isolated from these yeast strains were analyzed by the MFMAS, and their species were successfully identified with a similarity rate of 95% or higher. It was shown that the strains belonged to 43 different yeast species that are widely found in the foods. A clear discrimination was also observed in the phylogenetically related species. In conclusion, it might be suggested that the MFMAS-yeast seems to be a highly promising approach for a rapid, accurate, and one-step identification of the yeasts isolated from food products and/or their processing environments.

Update of the list of QPS‐recommended biological agents intentionally added to food or feed as notified to EFSA 10: Suitability of taxonomic units notified to EFSA until March 2019

“…These 30 articles included description of 45 studies related to different yeast species with QPS status, of which 16 referred to Candida kefyr (teleomorph = Kluyveromyces marxianus ) (Afsarian et al., ; Awad et al., ; Bharathi, ; Diba et al., ; García‐Agudo et al., ; Hasan and Yassein, ; Kaur et al., ; Kesmen et al., ; Khedri et al., ; Nagy et al., ; Okmen et al., ; Omran and Mansori, ; de Paula et al., ; Sadrossadati et al., ; Shokohi et al., ; Simi et al., ), 8 to Saccharomyces cerevisiae including Saccharomyces boulardii (corresponding to 7 articles: Lazo‐Vélez et al., ; Ruelle et al., ; Hasan and Yassein, ; Kara et al., ; Kesmen et al., ; Ochiai et al., ; Teblick et al., ), 10 to Candida famata (teleomorph = Debaryomyces hansenii ) (Afsarian et al., ; Alobaid and Khan, ; Awad et al., ; Cen et al., ; Das et al., ; Diba et al., ; Hasan and Yassein, ; Kesmen et al., ; de Paula et al., ; Simi et al., ), 6 to Candida pelliculosa (synonymus = Pichia anomala , teleomorph = Wickerhamomyces anomalus ) (Ahmadsah et al., ; Arendrup et al., ; Cen et al., ; Jung et al., ; Kesmen et al., ; Soman et al., ), 1 to Candida utilis (teleomorph = Lindnera jadinii ) (Treguier et al., ), 1 to Hanseniaspora uvarum (Kesmen et al., ), 1 Kluyveromyces lactis (Kesmen et al., ) a nd 1 to Schizosaccharomyces pombe (Kesmen et al., ). One of these articles was considered relevant (Pfaller et al., ) to evaluate since it presented new data on antimycotic MIC breakpoints for azoles but it is not associated to any specific yeast TU.…”