Assessment of molecular diversity and evolutionary relationship of kenaf (Hibiscus cannabinus L.), roselle (H. sabdariffa L.) and their wild relatives

Diversity analysis based on agro-morphological traits and microsatellite based markers in global germplasm collections of roselle (Hibiscus sabdariffa L.)

“…Cross species transferability of SSRs (50%; 8 out of 16 markers tested) was higher compared to ISSR markers (30.55%; 11 out of 36 markers tested) in roselle. Satya et al (2013) reported nearly similar transferability (48%) of jute specific SSRs in kenaf and roselle. The results are in conformity with Barbara et al (2007) that reported higher cross species transferability of SSRs within a taxonomic group.…”

“…The results are in conformity with Barbara et al (2007) that reported higher cross species transferability of SSRs within a taxonomic group. In contrast to Satya et al (2013) and Bakasso et al (2014) very high polymorphism of undertaken molecular markers (ISSR: 92.3%, SSR: 96.87%) recorded in the species that indicates broad genetic variability among accessions under study and polymorphic nature of these markers. Satya et al (2013) reported 60% (ISSR) and 47% (SSR) polymorphism in 11 genotypes of roselle, Bakasso et al (2014) reported 29.78% (AFLP) polymorphism in 124 accessions of roselle.…”

“…A total of 36 ISSR (specific to Musa sp., Solanum tuberosum. and Universal) (Muthusamy et al, 2008;Satya et al, 2012bSatya et al, , 2013Zhang et al, 2013) and 16 SSR markers specific to Hibiscus aridicola (Zhang et al, 2011) and H. rosa-sinensis (Bruna et al, 2008) were screened for amplification in roselle. Out of these only 11 ISSR (Table 2) and 8 SSR markers (Table 3) that showed reliable amplification were selected for final genotyping.…”

“…Out of these only 11 ISSR (Table 2) and 8 SSR markers (Table 3) that showed reliable amplification were selected for final genotyping. For ISSR analysis protocol of Satya et al (2013) was followed with some modifications. The PCR mixture (25 l) consisted of 4 l of genomic DNA (20 ng/l), 0.5 l of dNTPs (10 mM) (Genaxy, India), 1 l of primer (10 M) (Xcelris, India), 0.3 l of Taq polymerase (5U/l) (Genaxy, India) and 1 l of MgCl 2 (50 mM) in 2.5 l of 10X PCR Buffer.…”

“…Although, a range of DNA based molecular marker like RAPD (Cheng et al, 2002), AFLP (Cheng et al, 2004;Coetzee et al, 2008;Kim et al, 2010), SRAP , ISSR and SSR Satya et al, 2013;Satya et al, 2014) have been employed across globe to quantify genetic diversity in an another cultivated species of the genus namely H. cannabinus but only few such reports are available in roselle (Torres-Morán et al, 2011;Omalsaad et al, 2014;Satya et al, 2013Satya et al, , 2014) that too based on limited population size (11-32 accessions) except Bakasso et al (2014) which evaluated genetic diversity and population structure of 124 accessions of roselle from Niger, Africa.…”