Assessment of microbial pools by an innovative microbiological technique during the co-composting of olive mill by-products

Casacchia, Teresa; Toscano, Pietro; Sofo, Adriano; Perri, Enzo

doi:10.4236/as.2011.22015

Cited by 4 publications

(4 citation statements)

References 26 publications

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“…Because of the biologically-generated heat (bio-heat) and mechanical operations, bio-drying piles progress through mesophilic, thermophilic, and cooling phases (Cai et al, 2013), which are accompanied by the evolution of bacterial communities. The composition of bacterial populations during composting, a similar process based on aerobic fermentation, has been reported; e.g., Pseudomonas and Bacillus are the main decomposers of complex polymers, while Lutaonella thermophila can metabolize many organic acids and amino acids, and Ureibacillus contributes to the degradation of organic compounds, especially cellulosic material (Casacchia et al, 2011;Kuok et al, 2012). Despite the previous studies of the bacterial populations in compost, variations in the bacterial communities that play key roles in the bio-drying process have not been fully investigated.…”

Section: Introductionmentioning

confidence: 99%

Bacterial communities and their association with the bio-drying of sewage sludge

et al. 2016

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a b s t r a c tBio-drying is a technology that aims to remove water from a material using the microbial heat originating from organic matter degradation. However, the evolution of bacterial communities that are associated with the drying process has not been researched systematically. This study was performed to investigate the variations of bacterial communities and the relationships among bacterial communities, water evaporation, water generation, and organic matter degradation during the bio-drying of sewage sludge. High-throughput pyrosequencing was used to analyze the bacterial communities, while water evaporation and water generation were determined based on an in situ water vapor monitoring device. The values of water evaporation, water generation, and volatile solids degradation were 412.9 g kg À1 sewage sludge bio-drying material (SSBM), 65.0 g kg À1 SSBM, and 70.2 g kg À1 SSBM, respectively.Rarefaction curves and diversity indices showed that bacterial diversity plummeted after the temperature of the bio-drying pile dramatically increased on d 2, which coincided with a remarkable increase of water evaporation on d 2. Bacterial diversity increased when the pile cooled. During the thermophilic phase, in which Acinetobacter and Bacillus were the dominant genera, the rates of water evaporation, water generation, and VS degradation peaked. These results implied that the elevated temperature reshaped the bacterial communities, which played a key role in water evaporation, and the high temperature also contributed to the effective elimination of pathogens.

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Section: Introductionmentioning

confidence: 99%

Bacterial communities and their association with the bio-drying of sewage sludge

et al. 2016

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“…When the composting process entered the cooling stage, some exogenous microorganisms, including Pseudomonas , Ruminofilibacter , Devosia , Persicitalea , and Leadbetterella entered and proliferated in the samples. Pseudomonas is widely distributed in nature and can decompose complex polymers, such as lignocellulose [ 48 ], Ruminofilibacter can degrade xylan [ 49 ], and Devosia is a genus of cellulolytic bacteria [ 50 ]. These genera may contribute to lignocellulose degradation during DGW composting.…”

Section: Resultsmentioning

confidence: 99%

Development of a consortium-based microbial agent beneficial to composting of distilled grain waste for Pleurotus ostreatus cultivation

Zhou

et al. 2021

Biotechnol Biofuels

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Background Pleurotus ostreatus is an edible mushroom popularly cultivated worldwide. Distilled grain waste (DGW) is a potential substrate for P. ostreatus cultivation. However, components in DGW restrict P. ostreatus mycelial growth. Therefore, a cost-effective approach to facilitate rapid P. ostreatus colonization on DGW substrate will benefit P. ostreatus cultivation and DGW recycling. Results Five dominant indigenous bacteria, Sphingobacterium sp. X1, Ureibacillus sp. X2, Pseudoxanthomonas sp. X3, Geobacillus sp. X4, and Aeribacillus sp. X5, were isolated from DGW and selected to develop a consortium-based microbial agent to compost DGW for P. ostreatus cultivation. Microbial agent inoculation led to faster carbohydrate metabolism, a higher temperature (73.2 vs. 71.2 °C), a longer thermophilic phase (5 vs. 3 days), and significant dynamic changes in microbial community composition and diversity in composts than those of the controls. Metagenomic analysis showed the enhanced microbial metabolisms, such as xenobiotic biodegradation and metabolism and terpenoid and polyketide metabolism, during the mesophilic phase after microbial agent inoculation, which may facilitate the fungal colonization on the substrate. In accordance with the bioinformatic analysis, a faster colonization of P. ostreatus was observed in the composts with microbial inoculation than in control after composting for 48 h, as indicated from substantially higher fungal ergosterol content, faster lignocellulose degradation, and higher lignocellulase activities in the former than in the latter. The final mushroom yield shared no significant difference between composts with microbial inoculation and control, with 0.67 ± 0.05 and 0.60 ± 0.04 kg fresh mushroom/kg DGW, respectively (p > 0.05). Conclusion The consortium-based microbial agent comprised indigenous microorganisms showing application potential in composting DGW for providing substrate for P. ostreatus cultivation and will provide an alternative to facilitate DGW recycling.

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“…In a study done by Casacchia et al (2011), different mixtures of OP, OMWW, and OPR were aerobically cocomposted under natural conditions in an olive orchard located in southern Italy. During the experiment, compost temperature showed a sharp increase for the first 40 to 60 d, followed by stabilization at 60 C and a decline after 150 d; in contrast, compost water content ranged from 50 to 55% to 25 to 30%.…”

Section: Using In Situ Compost Productionmentioning

confidence: 99%

Sustainable Soil Management in Olive Orchards

Sofo¹,

Palese²,

Casacchia³

et al. 2014

Emerging Technologies and Management of Crop Stress Tolerance

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Assessment of microbial pools by an innovative microbiological technique during the co-composting of olive mill by-products

Cited by 4 publications

References 26 publications

Bacterial communities and their association with the bio-drying of sewage sludge

Bacterial communities and their association with the bio-drying of sewage sludge

Development of a consortium-based microbial agent beneficial to composting of distilled grain waste for Pleurotus ostreatus cultivation

Sustainable Soil Management in Olive Orchards

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