Assessment of Microbial Communities and Their Relationship with Enzymatic Activity during Composting

Kazemi, Khoshrooz; Zhang, Baiyu; Lye, Leonard M.

doi:10.4236/wjet.2017.53b011

Cited by 5 publications

(4 citation statements)

References 16 publications

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“…The concentration of the protons exchanged during the oxidation-reduction processes was calculated from the concentration of the reduced 2,3,5-formazan [29]. DHA is an indicator of the total rate of cell metabolism and is directly proportional to the effectiveness and the rate of biodegradation of the organic matter [30][31][32][33].…”

Section: Analyzed Parameters and Methodsmentioning

confidence: 99%

Assessment of Denitrification and Nitrification Processes during Landfill Leachate Treatment

Schneider,

Yotinov,

Dinova

et al. 2023

Processes

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Landfill leachate is generated from waste degradation in landfill sites, and its treatment includes biological or combined treatment with physico-chemical methods. A frequently applied technology in the biological stage of landfill leachate treatment plants is based on denitrification and nitrification. Nevertheless, with the availability of a huge number of scientific reports, the management of denitrification and nitrification (D/N) processes in the frame of real wastewater treatment plants (WWTPs) is rather difficult and always remains a critical technological problem. This study aims to perform a comparative assessment between denitrification and nitrification processes during landfill leachate treatment in three different situations in the WWTP of the Municipal Enterprise for Waste Treatment in Sofia City, Bulgaria. The comparative assessment is based on the biotransformation effectiveness of ammonium ions in the course of nitrification and of nitrates in the course of denitrification. Mixed samples (wastewater and activated sludge) were taken from the sequencing batch reactors operating in the mode of denitrification and nitrification. All physico-chemical (pH, dissolved oxygen, organics concentration, BOD5:COD, nitrogen and phosphorous) and biological (sludge volume index, sludge biotic index, quantity of aerobic heterotrophs, denitrifiers and nitrifiers, total dehydrogenase activity) indicators were investigated in the samples from the biobasins in the mode of denitrification and nitrification. The conditions for the implementation of the two processes were compared, along with the state of the activated sludge in the different reactors. The obtained results showed that denitrification was the critical process in the technology on the base of D/N. A major factor that managed the denitrification was the lower concentration of biodegradable organic matter which led to a deformation of the activated sludge structure, a decrease in the count of heterotrophic microorganisms, a decrease in the total activity of the activated sludge and an inhibition of the nitrate reductase activity. The nitrification processes were accomplished with high intensity. The results confirmed that D/N could be optimized and controlled using specific wastewater treatment technology parameters and purposely applied indicators.

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Section: Analyzed Parameters and Methodsmentioning

confidence: 99%

Assessment of Denitrification and Nitrification Processes during Landfill Leachate Treatment

Schneider,

Yotinov,

Dinova

et al. 2023

Processes

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show abstract

“…All extracts and liquid samples were analyzed for metal concentration using an ICP-MS spectrometer (model Thermo iCAP TQ from ThermoFisher Scientific, Waltham, MA USA). Soil dehydrogenase enzyme was analyzed by reducing triphenyl-tetrazolium chloride to triphenyl-formazan and measuring the obtained solution photometrically at 546 nm [25,26].…”

Section: Sampling and Chemical Analysismentioning

confidence: 99%

Comprehensive study of electrokinetic-assisted phytoextraction of metals from mine tailings by applying direct and alternate current

Medina-Díaz

López‐Bellido

Alonso-Azcárate

et al. 2023

Electrochimica Acta

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“…Dehydrogenase activity (DH) was determined using the 2,3,5-Triphenyltetrazolium chloride (TTC) method according to [48] immediately after sampling. Briefly, approximately 5 g of frozen sediment was incubated with 5 mL of TTC solution (1%).…”

Section: Dehydrogenase and Extracellular Enzymatic Activities (Eeas)mentioning

confidence: 99%

Impact of Elevated Atmospheric CO2 in Spartina maritima Rhizosphere Extracellular Enzymatic Activities

Duarte

Baeta

Marques

et al. 2023

Water

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Atmospheric CO2 enrichment, which is caused to a large extent by anthropogenic activities, is known to interfere with sediment microbial communities via plant rhizospheres. The present work aimed to evaluate this interaction in Spartina maritima ((Curtis) Fernald.) rhizosediments, aiming to depict the impacts of atmospheric CO2 increase in the biogeochemical processes occurring in the rhizosphere of this pioneer and highly abundant Mediterranean halophyte. For this purpose, mesocosms trials were conducted, exposing salt marsh cores with S. maritima and its sediments to 410 and 700 ppm of CO2 while assessing rhizosediment extracellular enzymatic activities. An evident increase in dehydrogenase activity was observed and directly linked to microbial activity, indicating a priming effect in the rhizosphere community under increased CO2. Phosphatase showed a marked increase in rhizosediments exposed to elevated CO2, denoting a higher requirement of phosphate for maintaining higher biological activity rates. High sulphatase activity suggests a possible S-limitation (microbial or plant) due to elevated CO2, probably due to higher sulphur needs for protein synthesis, thus increasing the need to acquire more labile forms of sulphur. With this need to acquire and synthesize amino acids, a marked decrease in protease activity was detected. Most carbon-related enzymes suffered an increase under increased CO2. Overall, a shift in sediment extracellular enzymatic activity could be observed upon CO2 fertilization, mostly due to priming effects and not due to changes in the quality of carbon substrates, as shown by the sediment stable isotope signatures. The altered recycling activity of organic C, N, and P compounds may lead to an unbalance of these biogeochemical cycles, shifting the rhizosphere ecosystem function, with inevitable changes in the ecosystem services level.

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Assessment of Microbial Communities and Their Relationship with Enzymatic Activity during Composting

Cited by 5 publications

References 16 publications

Assessment of Denitrification and Nitrification Processes during Landfill Leachate Treatment

Assessment of Denitrification and Nitrification Processes during Landfill Leachate Treatment

Comprehensive study of electrokinetic-assisted phytoextraction of metals from mine tailings by applying direct and alternate current

Impact of Elevated Atmospheric CO2 in Spartina maritima Rhizosphere Extracellular Enzymatic Activities

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