Assessment of microbial activity and bacterial community composition in the rhizosphere of a copper accumulator and a non-accumulator

Wang, Yuanpeng; Li, Qingbiao; Shi, Jiyan; Qiu, Lin; Chen, Xincai; Wu, Weixiang; Chen, Yingxu

doi:10.1016/j.soilbio.2007.12.010

Cited by 94 publications

(43 citation statements)

References 30 publications

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“…The DGGE profile has shown that the structure of the bacterial community changed in heavy metal-amended samples. Similarly, Wang et al (2008) has also reported that the bacterial community structure was changed in the soils amended with Cu and cultivated with different plants. In our previous work, microbial community structure was also changed in heavy metal-amended soils (Khan et al 2007).…”

Section: Discussionmentioning

confidence: 85%

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Effects of Cd and Pb on soil microbial community structure and activities

Khan

Hesham

Qiao

et al. 2009

Environ Sci Pollut Res

319

132

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Background, aim, and scope Soil contamination with heavy metals occurs as a result of both anthropogenic and natural activities. Heavy metals could have long-term hazardous impacts on the health of soil ecosystems and adverse influences on soil biological processes. Soil enzymatic activities are recognized as sensors towards any natural and anthropogenic disturbance occurring in the soil ecosystem. Similarly, microbial biomass carbon (MBC) is also considered as one of the important soil biological activities frequently influenced by heavy metal contamination. The polymerase chain reaction-denaturing gradient gel electrophoresis (DGGE) has recently been used to investigate changes in soil microbial community composition in response to environmental stresses. Soil microbial community structure and activities are difficult to elucidate using single monitoring approach; therefore, for a better insight and complete depiction of the soil microbial situation, different approaches need to be used. This study was conducted in a greenhouse for a period of 12 weeks to evaluate the changes in indigenous microbial community structure and activities in the soil amended with different application rates of Cd, Pb, and Cd/Pb mix. In a field environment, soil is contaminated with single or mixed heavy metals; so that, in this research, we used the selected metals in both single and mixed forms at different application rates and investigated their toxic effects on microbial community structure and activities, using soil enzyme assays, plate counting, and advanced molecular DGGE technique. Soil microbial activities, including acid phosphatase (ACP), urease (URE), and MBC, and microbial community structure were studied. Materials and methods A soil sample (0-20 cm) with an unknown history of heavy metal contamination was collected and amended with Cd, Pb, and Cd/Pb mix using the CdSO 4 and Pb(NO 3 ) 2 solutions at different application rates. The amended soils were incubated in the greenhouse at 25±4°C and 60% water-holding capacity for 12 weeks. During the incubation period, samples were collected from each pot at 0, 2, 9, and 12 weeks for enzyme assays, MBC, numeration of microbes, and DNA extraction. Fumigationextraction method was used to measure the MBC, while plate counting techniques were used to numerate viable heterotrophic bacteria, fungi, and actinomycetes. Soil DNAs were extracted from the samples and used for DGGE analysis. Results ACP, URE, and MBC activities of microbial community were significantly lower (p <0.05) in the metal-amended samples than those in the control. The enzyme inhibition extent was obvious between different incubation periods and varied as the incubation proceeded, and the highest rate was detected in the samples after 2 weeks. However, the lowest values of ACP and URE activities (35.6% and 36.6% of the control, respectively) were found in the Cd 3 /Pb 3 -treated sample after 2 weeks. Similarly, MBC was strongly decreased in both Cd/Pbamended samples and highest reduction (52.4%) was Envir...

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Section: Discussionmentioning

confidence: 85%

“…These techniques have recently been used to investigate changes in soil microbial community composition (Claudia et al 2003;Oliveira and Pampulha 2006;Wang et al 2008;Moreels et al 2008). The DGGE technique is used for screening microbial community composition and also changes in response to environmental stresses (Mette and Neils 2002).…”

mentioning

confidence: 99%

Effects of Cd and Pb on soil microbial community structure and activities

Khan

Hesham

Qiao

et al. 2009

Environ Sci Pollut Res

319

132

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“…Heavy metals in bioavailable forms adversely affect soil microbes by reducing their populations and changing the community structure and diversity (Renella et al 2005). The adverse impacts are reflected in terms of decreased soil enzyme activities (Belyaeva et al 2005) and interferences in plant-soil-metals association (Wang et al 2008). Kandeler et al (1996) noted that heavy metals reduce functional diversity of the affected soil microbial communities.…”

Section: Introductionmentioning

confidence: 99%

Influence of Biochar on Microbial Activities of Heavy Metals Contaminated Paddy Fields

Cui¹,

Yan²,

Yang³

et al. 2013

BioResources

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dBiochar (BC) amendments might decrease the bioavailability of metals in soils that are contaminated with heavy metals. In general, soil microbial communities are sensitive to changes in soil property changes. Microbial communities were tested in a Cd-and Pb-polluted paddy field in southern China. BC was applied as a basal soil amendment before rice transplantation in 2009. The BC was applied at rates of 0, 10, 20, and 40 tons per hectare. Soil heavy metal fractions with sequential extraction procedure, soil microorganisms, and enzymes were monitored in 2011. The soil pH and soil organic carbon (SOC) were significantly increased by 2% to 5% and 16% to 51% under BC amendment, respectively. Compared to the non-BC treatment, the cadmium (Cd) and lead (Pb) acid-soluble fraction concentrations were significantly decreased by 15.3% to 26.7% and 18.2% to 30.9%. The Cd and Pb reducible fraction were decreased by 13.5% to 25.6% and 21.9% to 23.53%.The Cd and Pb oxidizable fraction by 15.4% to 69.2% and 22.7% to 29.3% with BC application, respectively. The populations of actinomycetes and fungi were increased by 19.0% to 38.5% and 3.7 to 9.3 times, respectively. Meanwhile, BC significantly increased the cellulose, urine enzyme, neutral phosphatase, and sucrase activities by 117.4% to 178.3%, 31.1% to 37.6%, 29.7% to 193.8%, and 36.5% to 328.6%, respectively. BC amendment offers a basic option to reduce Cd and Pb bioavailability and change the fractions. The BC also increases microorganism quantity and soil enzyme activity.

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“…The conventional phytoremediation way has focused on metal hyperaccumulator or accumulator plants, which can grow in heavy metal contaminated soils, and is capable of accumulating potentially phytotoxic elements to concentrations much higher than the normal plants growing in the same environment [1], [3], [5]. At present, more than 400 species of accumulator plants have been found [4], [5].…”

Section: Introductionmentioning

confidence: 99%

“…At present, more than 400 species of accumulator plants have been found [4], [5]. However, most of these accumulator plants usually had very high heavy metal contents, so after harvested the plants usually need incineration treatment [4].…”

Section: Introductionmentioning

confidence: 99%

Study on Tolerance and Accumulation Potential of Biofuel Crops for Phytoremediation of Heavy Metals

Oh¹,

Li²,

Hong-yan³

et al. 2013

IJESD

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Abstract-Phytoremediation is a cost-effective and environment-friendly technology that uses plants for environmental remediation. In order to promote practical application of phytoremediation, an income generation phytoremediation strategy was suggested using biofuel crops for utilization and phytoremediation of contaminated soils. In this study, the tolerance and accumulation potential to heavy metals of two biofuel plants and two accumulator plants were investigated in order to evaluate their suitability for phytoremediation. Two biofuel crops, maize (Zea mays L.) and sunflower (Helianthus annuus L.), and two accumulator plants, Elsholtzia splendens (ES) and Kummerowia striata (KS) were exposed to the hydroponic mixtures of Pb, Cu and Cd. Results showed that, at the 0.01 mol/L application level of Pb, Cu and Cd, sunflower had the highest accumulation ability for Pb, Cu and Cd in the shoot part compared to other three plants, and maize had a similar accumulation level to ES and KS. Sunflower also had much higher shoot/root ratios of accumulation for Cu and Cd than maize. However, sunflower showed lower tolerance to Pb, Cu and Cd contamination than maize, ES and KS. This study provided useful data supporting the use of biofuel plants in phytoremediation.

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Assessment of microbial activity and bacterial community composition in the rhizosphere of a copper accumulator and a non-accumulator

Cited by 94 publications

References 30 publications

Effects of Cd and Pb on soil microbial community structure and activities

Effects of Cd and Pb on soil microbial community structure and activities

Influence of Biochar on Microbial Activities of Heavy Metals Contaminated Paddy Fields

Study on Tolerance and Accumulation Potential of Biofuel Crops for Phytoremediation of Heavy Metals

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