Assessment of Metal - Induced Inhibition of Soybean Urease as a Tool for Measuring Heavy Metals in Aqueous Samples

Magomya, A. M.; Barminas, J. T.; Osemeahon, S. A.

doi:10.9790/5736-1006026170

Cited by 2 publications

(1 citation statement)

References 28 publications

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“…Reported order of degree of inhibitions for soil urease by various heavy metals is: Ag > Hg > Cu > Ni > Co > Cd > Fe > Zn > Pb (Preininger and Wolfbeis 1996). Another study suggested the inhibition order as Cu 2+ > As 3+ > Cr 3+ > Cd 2+ > Zn 2+ > Pb 2+ in case of urease extracted from soybeans (Magomya et al 2017). Enzyme inhibition by heavy metals is a well-studied research area.…”

Section: Cr 6+ and Fe 2+mentioning

confidence: 99%

Inhibition assays of free and immobilized urease for detecting hexavalent chromium in water samples

et al. 2019

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The present work describes the inhibition studies of free as well as immobilized urease by different heavy metals. Porous silicon (PS) films prepared by electrochemical etching were used for urease immobilization by physical adsorption. The enzyme was subjected to varying concentrations of Cr 6+ , Cr 3+ , Cu 2+ , Fe 2+ , Cd 2+ and Ni 2+ and analyzed for the variation in the activity. To study the effect of other heavy metals on the interaction of urease and Cr 6+ , free as well as immobilized urease was subjected to the combination of each metal ion with Cr 6+. Results proved the sensitivity of free as well as immobilized urease towards heavy metals by observed reduction in activity. Immobilized urease showed less degree of inhibition compared to free urease when tested for inhibition by individual metal ions and in combination with Cr 6+. IC 50 values were found higher for inhibition by the combination of metal ions with Cr 6+. Interaction of heavy metal ions with functional groups in active site of urease and limitations of mass transfer are the two factors responsible for the variation in activity of urease. Relation between the variation of urease activity and amount of heavy metals can be applied in biosensor development for determining the concentration of Cr 6+ present in the water samples.

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Section: Cr 6+ and Fe 2+mentioning

confidence: 99%