Assessment of membrane toxicity using [3H]arachidonic acid release in U937 cells

Klöcking, H.-P.; Schlegelmilch, Ulf; Klöcking, R.

doi:10.1016/0887-2333(94)90065-5

Cited by 14 publications

(3 citation statements)

References 2 publications

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“…Histological study is used to evaluate RHOE alterations due to cytotoxic or inflammatory effects of RAMEB. The results are compared to tissue exposure to active control 1% SDS which is known to be extensively toxic 28–30. Buccal epithelial cell turnover being about 5–6 days, repeated exposures of RHOE to RAMEB are carried out during 5 days to mimic as much as possible in vivo conditions.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Buccal Methyl-β-Cyclodextrin Toxicity on Human Oral Epithelial Cell Culture Model

Boulmedarat

Bochot

Lesieur

et al. 2005

Journal of Pharmaceutical Sciences

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Section: Discussionmentioning

confidence: 99%

Evaluation of Buccal Methyl-β-Cyclodextrin Toxicity on Human Oral Epithelial Cell Culture Model

Boulmedarat

Bochot

Lesieur

et al. 2005

Journal of Pharmaceutical Sciences

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“…SDS is an anionic detergent, known to disrupt membrane integrity (Gould et al 2000, Klocking et al 1994), and is, like cadmium, copper, and lead, a U.S. Environmental Protection Agency‐approved reference contaminant (Klemm et al 1994). The 4‐h IC 50 concentration of SDS needed to inhibit the recovery of bioluminescence in P. lunula by 50% fits well with other reported sensitivities and variability in various organisms (Bierkens et al 1998, Gormly et al 1996, Gulec et al 1997, Hooten and Carr 1998, Kraus et al 1998, Langdon et al 1996, Lussier et al 1999, Nalecz‐Jawecki et al 1997, Villegas‐Navarro et al 1999).…”

Section: Discussionmentioning

confidence: 99%

Effects of Metals and Organic Contaminants on the Recovery of Bioluminescence in the Marine Dinoflagellate Pyrocystis Lunula (Dinophyceae)1

Heimann

Matuszewski

Klerks

2002

Journal of Phycology

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Pyrocystis lunula Schütt is a unicellular photoautotrophic dinoflagellate, commonly found in marine environments, displaying circadian‐controlled bioluminescence. Because of this species' characteristics, effects of pollutants on bioluminescence in P. lunula may make for an easy and simple bioassay that would be valuable for toxicity testing and the protection of coastal resources. This study therefore investigated the short‐term effects of metals and organic pollutants on the recovery of the bioluminescent potential in P. lunula. Recovery of bioluminescence was strongly inhibited in a dose‐dependent manner by all reference contaminants tested, the system being most sensitive to copper and cadmium (4‐h IC50s 0.96 and 1.18 μM, respectively), followed by phenanthrene, lead, SDS, and nickel (4‐h IC50s 1.64, 12.8, 15.6, and 73.1 μM, respectively), whereas relatively high concentrations of phenol were needed to elicit a response (4‐h IC50 1.64 mM). Except for exposure to lead and nickel, the inhibitory effects of cadmium, copper, and all organic pollutants were reversible, with P. lunula recovering 80%–100% of its bioluminescence potential after a period of 72 h in uncontaminated medium. Our results show that the restoration of bioluminescence in P. lunula is sensitive to the reference contaminants tested and obtains highly reproducible results.

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“…SDS is an anionic detergent, known to disrupt membrane integrity (Gould et al 2000, Klocking et al 1994, and is, like cadmium, copper, and lead, a U.S. Environmental Protection Agency-approved reference contaminant (Klemm et al 1994). The 4-h IC 50 concentration of SDS needed to inhibit the recovery of bioluminescence in P. lunula by 50% fits well with other reported sensitivities and variability in various organisms (Bierkens et al 1998, Gormly et al 1996.…”

Section: Discussionmentioning

confidence: 99%

Effects of Metals and Organic Contaminants on the Recovery of Bioluminescence in the Marine Dinoflagellate Pyrocystis Lunula (Dinophyceae)1

Heimann¹,

Matuszewski²,

Klerks³

2002

J Phycol

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Pyrocystis lunulaSchütt is a unicellular photoautotrophic dinoflagellate, commonly found in marine environments, displaying circadian-controlled bioluminescence. Because of this species' characteristics, effects of pollutants on bioluminescence in P. lunula may make for an easy and simple bioassay that would be valuable for toxicity testing and the protection of coastal resources. This study therefore investigated the short-term effects of metals and organic pollutants on the recovery of the bioluminescent potential in P. lunula. Recovery of bioluminescence was strongly inhibited in a dose-dependent manner by all reference contaminants tested, the system being most sensitive to copper and cadmium (4-h IC 50 s 0.96 and 1.18 M, respectively), followed by phenanthrene, lead, SDS, and nickel (4-h IC 50 s 1.64, 12.8, 15.6, and 73.1 M, respectively), whereas relatively high concentrations of phenol were needed to elicit a response (4-h IC 50 1.64 mM). Except for exposure to lead and nickel, the inhibitory effects of cadmium, copper, and all organic pollutants were reversible, with P. lunula recovering 80%-100% of its bioluminescence potential after a period of 72 h in uncontaminated medium. Our results show that the restoration of bioluminescence in P. lunula is sensitive to the reference contaminants tested and obtains highly reproducible results.

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Assessment of membrane toxicity using [3H]arachidonic acid release in U937 cells

Cited by 14 publications

References 2 publications

Evaluation of Buccal Methyl-β-Cyclodextrin Toxicity on Human Oral Epithelial Cell Culture Model

Evaluation of Buccal Methyl-β-Cyclodextrin Toxicity on Human Oral Epithelial Cell Culture Model

Effects of Metals and Organic Contaminants on the Recovery of Bioluminescence in the Marine Dinoflagellate Pyrocystis Lunula (Dinophyceae)1

Effects of Metals and Organic Contaminants on the Recovery of Bioluminescence in the Marine Dinoflagellate Pyrocystis Lunula (Dinophyceae)1

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