Assessment of maturation status of tumor-infiltrating dendritic cells in invasive ductal carcinoma of the breast: relation with vascular endothelial growth factor expression

Deeb, Nevine M.F. El; Mehanna, Radwa A.

doi:10.5146/tjpath.2013.01186

Cited by 5 publications

(8 citation statements)

References 26 publications

(34 reference statements)

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“…Thus, the increased osmotic fragility of mDCs is more sensitive, and the decreased hypo-osmolality resistance of mDCs induced by VEGF cytokine may lead to their cytolysis in TME. This might be one of the reasons for less infiltration of mDCs in tumor tissue and draining lymph nodes and/or for a decline of local immune response in tumors [46,47]. …”

Section: Resultsmentioning

confidence: 99%

“…In addition, the contact area between mDCs and naive T cells would became smaller as the reduced deformability, which might impair the formation of immune synapses and eventually reduce the activation efficiency of T cells in lymph node. VEGF expression is correlated directly with positive nodal metastases and correlated inversely with the count of mDCs [47], and thus, there are different degrees of influence on mDCs in various tumor patients.…”

Section: Resultsmentioning

confidence: 99%

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Biophysical Properties and Motility of Human Mature Dendritic Cells Deteriorated by Vascular Endothelial Growth Factor through Cytoskeleton Remodeling

Xue

Long

et al. 2016

IJMS

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Dendritic cells (DCs), the most potent antigen-presenting cells, play a central role in the initiation, regulation, and maintenance of the immune responses. Vascular endothelial growth factor (VEGF) is one of the important cytokines in the tumor microenvironment (TME) and can inhibit the differentiation and functional maturation of DCs. To elucidate the potential mechanisms of DC dysfunction induced by VEGF, the effects of VEGF on the biophysical characteristics and motility of human mature DCs (mDCs) were investigated. The results showed that VEGF had a negative influence on the biophysical properties, including electrophoretic mobility, osmotic fragility, viscoelasticity, and transmigration. Further cytoskeleton structure analysis by confocal microscope and gene expression profile analyses by gene microarray and real-time PCR indicated that the abnormal remodeling of F-actin cytoskeleton may be the main reason for the deterioration of biophysical properties, motility, and stimulatory capability of VEGF-treated mDCs. This is significant for understanding the biological behavior of DCs and the immune escape mechanism of tumors. Simultaneously, the therapeutic efficacies may be improved by blocking the signaling pathway of VEGF in an appropriate manner before the deployment of DC-based vaccinations against tumors.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Biophysical Properties and Motility of Human Mature Dendritic Cells Deteriorated by Vascular Endothelial Growth Factor through Cytoskeleton Remodeling

Xue

Long

et al. 2016

IJMS

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“…On the contrary, high abundance (above the median) of resting DCs is associated with worse prognosis [ 26 ]. Several molecules expressed or secreted by BC cells, such as polyamines [ 23 ], growth factors [ 27 , 28 ], interleukins and cytokines [ 28 , 29 ], were found to affect DCs properties.…”

Section: Discussionmentioning

confidence: 99%

“…Lopez et al observed high densities of these and DC-LAMP + cell populations in high-grade triple-negative BC [ 32 ]. In contrast, other authors suggested that there are associations between more mature DCs and low-grade IBC [ 27 ] or no relationship between CD1a + DCs and IBC grade [ 27 , 31 ]. Therefore, it appears that the distribution of DCs subsets in tumors of different grades remains similar during the transition from DCIS to IBC.…”

Section: Discussionmentioning

confidence: 99%

Dendritic Cell Subpopulations Are Associated with Morphological Features of Breast Ductal Carcinoma In Situ

Szpor,

Streb,

Glajcar

et al. 2023

IJMS

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Ductal carcinoma in situ (DCIS) is the preinvasive form of breast cancer (BC). It is disputed whether all cases of DCIS require extensive treatment as the overall risk of progression to BC is estimated at 40%. Therefore, the crucial objective for researchers is to identify DCIS with significant risk of transformation into BC. Dendritic cells (DC) are professional antigen presenting cells and as such play a pivotal role in the formation of immune cells that infiltrate in breast tumors. The aim of this study was to investigate the relationship between the density of DCs with different superficial antigens (CD1a, CD123, DC-LAMP, DC-SIGN) and various histopathological characteristics of DCIS. Our evaluation indicated that CD123+ and DC-LAMP+ cells were strongly associated with maximal tumor size, grading and neoductgenesis. Together with CD1a+ cells, they were negatively correlated with hormonal receptors expression. Furthermore, the number of DC-LAMP+ cells was higher in DCIS with comedo necrosis, ductal spread, lobular cancerization as well as comedo-type tumors, while CD1a+ cells were abundant in cases with Paget disease. We concluded that different subpopulations of DCs relate to various characteristics of DCIS. Of the superficial DCs markers, DC-LAMP seems particularly promising as a target for further research in this area.

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“…It has previously been reported that incubation of tumor-associated DCs with VEGF and oncostatin M can direct progenitor cell differentiation away from the DC pathway to an ELC fate ( 11 ). Furthermore, the tumor microenvironment can induce the ELD of iDCs, but not of mature DCs ( 22 – 24 ). While these previous studies have examined the effect of DC differentiation stage on the transition to ELCs, to the best of our knowledge, there is no previous study that addresses whether the degree of differentiation of tumor cells can influence the ELD of iDCs.…”

Section: Discussionmentioning

confidence: 99%

JAK/STAT3 signaling pathway mediates endothelial-like differentiation of immature dendritic cells

et al. 2015

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Endothelial-like differentiation (ELD) of dendritic cells (DCs) is a poorly understood phenomenon. The present study evaluated the effect on the ELD of DCs by using human esophageal squamous cell carcinoma (ESCC) cells with high or poor differentiation. The results demonstrated that KYSE450 (highly differentiated) and KYSE70 (poorly differentiated) cell supernatants induce the differentiation of immature DCs (iDCs), derived from healthy adult volunteers, away from the DC pathway and towards an endothelial cell (EC) fate. This effect was strongest in the cells treated with the KYSE70 supernatant. During the ELD of iDCs, sustained activation of JAK (janus tyrosine kinase)/STAT3 (signal transducer and activator of transcription 3) signaling was detected. Incubation of iDCs with the JAK inhibitor, AG490 blocked JAK/STAT3 phosphorylation and iDC differentiation. These results suggested that the JAK/STAT3 signaling pathway mediates ELD of iDCs. Furthermore, the poorly differentiated ESCC cells may have a greater effect on the ELD of iDCs than highly differentiated ESCC cells.

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Assessment of maturation status of tumor-infiltrating dendritic cells in invasive ductal carcinoma of the breast: relation with vascular endothelial growth factor expression

Cited by 5 publications

References 26 publications

Biophysical Properties and Motility of Human Mature Dendritic Cells Deteriorated by Vascular Endothelial Growth Factor through Cytoskeleton Remodeling

Biophysical Properties and Motility of Human Mature Dendritic Cells Deteriorated by Vascular Endothelial Growth Factor through Cytoskeleton Remodeling

Dendritic Cell Subpopulations Are Associated with Morphological Features of Breast Ductal Carcinoma In Situ

JAK/STAT3 signaling pathway mediates endothelial-like differentiation of immature dendritic cells

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