Assessment of long term ecotoxicity of urban stormwaters using a multigenerational bioassay on Ceriodaphnia dubia: A preliminary study

Abstract

Standardized ecotoxicity bioassays show some limits to assess properly long-term residual toxicity of complex mixture of pollutants often present at low concentration, such as stormwaters. Among invertebrate organisms used for ecotoxicity testing, the microcrustacean Ceriodaphnia dubia (C. dubia) is considered as one of the most sensitive, especially regarding reproduction impairment as a toxicity endpoint. Consequently, this work explores the interest to perform a multigenerational assay based on the study of the reproduction of C. dubia to assess long-term ecotoxicity of complex mixture, using stormwater samples. With this in mind, a battery of standardized bioassays (Daphnia magna mobility, Pseudokirchneriella subcapitata population growth, Heterocypris incongruens growth and one generation C. dubia reproduction inhibition assays) was performed in parallel to a three generation C. dubia reproduction inhibition assay on 2 stormwater samples. Results highlighted that while all standardized bioassays failed to reveal residual toxicity in the stormwater samples, the C. dubia multigenerational assay exhibited an higher sensitivity than the previous ones. No adverse effect was observed for the first exposed generation, but an increase in mortality and a reproduction disturbance was obtained in the second and third exposed generation depending of the sample. Further experiments are now needed to optimize the exposure protocol of this multigenerational assay.

Potential Impacts of Oil and Grease on Algae, Invertebrates and Fish in the Bujagali Hydropower Project Area

“…Heterocypris incongruens); and reproduction of one generation of a water flea in the class Branchiopoda (i.e. Ceriodaphnia dubia) (Gosset et al, 2018). These scholars however, did not observe any adverse effect on the first exposed generation, but an increase in mortality and a reproduction disturbance was obtained in the second and third exposed generations.…”

“…These scholars however, did not observe any adverse effect on the first exposed generation, but an increase in mortality and a reproduction disturbance was obtained in the second and third exposed generations. The same scholars (Gosset et al, 2018) also undertook a preliminary assessment of long term eco toxicity of urban storm waters using a multigenerational bioassay on Ceriodaphnia dubia. This micro crustacean (C. dubia) is considered one of the most sensitive, especially regarding reproduction impairment as a toxicity endpoint.…”

Macroinvertebrados aquáticos como bioindicadores do impacto do cultivo de cana-de-açúcar no Estado de São Paulo: estrutura da comunidade e toxicidade dos sedimentos

“…Em peixes, a fertilidade dos adultos na geração P e a eclodibilidade na geração F1 foi reduzida em exposição a diferentes pesticidas (HOLDWAY et al, 2008). Para Ceriodaphnia dubia, não foram observados efeitos adversos para a primeira geração, mas perturbações na reprodução começaram a ser observadas a partir da segunda e terceira geração, em exposição às águas pluviais urbanas (GOSSET et al, 2018). Oliveira-Filho et al (2009) também mostraram efeitos adversos da exposição de endosulfan na produção de ovos e na massa de ovos colocadas pela geração F0 de caracóis, além de observarem efeitos de má formação e redução da eclosão da geração seguinte.…”