Assessment of lncRNA DANCR, miR-145-5p and NRAS axis as biomarkers for the diagnosis of colorectal cancer

Bahreini, Fatemeh; Saidijam, Massoud; Mousivand, Zahra; Najafi, Rezvan; Afshar, Saeid

doi:10.1007/s11033-021-06373-2

Cited by 9 publications

(4 citation statements)

References 29 publications

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“…Recent evidence has shown that lncRNAs have the ability to regulate complex and diverse biological functions, and they have important significance for different tumor types and for diagnosis, treatment and prognosis [ 14 , 15 ]. In the last few years, multiple studies have established DANCR roles in promoting cancer tumor growth and migration among different cancers [ 16 , 17 ].…”

Section: Discussionmentioning

confidence: 99%

“…DANCR, as a lncRNA, has many targets or targets, which might regulate CRC in different ways. Previous researches have reported that DANCR, as biomarkers for the diagnosis of colorectal cancer, might facilitate the growth and metastasis of CRC by regulating the epithelial-mesenchymal transition (EMT) process [ 14 , 24 ]. Consistently, our results showed that miR-508-5p targets DANCR directly through bioinformatics analysis and dual-luciferase analysis.…”

Section: Discussionmentioning

confidence: 99%

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Activation of lncRNA DANCR by H3K27 acetylation regulates proliferation of colorectal cancer cells

Han,

Shan,

Huang

et al. 2024

Discov Onc

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The long noncoding DANCR functions as a tumor oncogene in many cancers, including colorectal cancer (CRC). However, the molecular mechanism of DANCR in CRC has not been explored. This study probed the function and potential mechanism by which DANCR contributes to the progression of CRC. The obtained data indicated that DANCR is overexpressed in CRC tissues and cell lines. Knockdown of DANCR hindered CRC cell proliferation, which was mediated by cyclin D1 and CDK4. Bioinformatic analysis, luciferase reporter assays and subcellular fractionation verified that DANCR directly binds to miR-508-5p. Moreover, DANCR acts as a miR-508-5p ceRNA to regulate expression of ATF1. In addition, upregulation of DANCR is attributed to H3K27 acetylation at the promoter region. In conclusion, our study confirmed that activation of lncRNA DANCR by H3K27 acetylation has an oncogenic role in CRC progression and provides a potential therapeutic target for CRC.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Activation of lncRNA DANCR by H3K27 acetylation regulates proliferation of colorectal cancer cells

Han,

Shan,

Huang

et al. 2024

Discov Onc

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“…Additionally, functional experiments showed that miR-145-5p attenuated proliferation, migration and invasion in the Eca109 cell line. In fact, much evidence has revealed that miR-145-5p functioned as a tumor suppressor and that the expression levels of miR-145-5p were extremely downregulated in certain types of cancers to avoid antioncogenic functions ( 10 , 17 , 19 - 21 ). The results of our study also show that the miR-145-5p level is significantly associated with the progression of ESCC.…”

Section: Discussionmentioning

confidence: 99%

The role of miR-145-5p in esophageal squamous cell carcinoma tumor-associated macrophages and selection of immunochemotherapy

Lin¹,

Wu²,

Zhu³

et al. 2022

J Thorac Dis

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Background The impact of miR-145-5p in immune infiltration and the potential application in esophageal squamous cell carcinoma (ESCC) immunochemotherapy remains unknown. Methods Transcriptomic data for ESCC tissues and normal tissues and clinical materials of patients with ESCC were obtained from The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) databases. The differences in mRNA levels in cancer tissues and noncancerous tissues were analyzed, and we subsequently investigated the association between miR-145-5p expression and the key parameters of ESCC progression and prognosis. Additionally, cytological experiments were performed to evaluate the biological functions of miR-145-5p. Pathways potentially affected by miR-145-5p were analyzed by Gene Set Enrichment Analysis (GSEA) and REACTOME. We also analyzed the function of miR-145-5p in immune infiltration through the TIMER2 (Tumor Immune Estimation Resource) database. Results The analysis of gene chip data from the TCGA database and GEO database (including GSE13937 and GSE43732) showed that the expression of miR-145-5p is downregulated in ESCC (P<0.05) and that patients with high miR-145-5p levels had lower survival rates (P<0.05). The expression of miR-145-5p was significantly correlated with the disease-free survival (DFS) rate (P<0.05) and M stage (P<0.05) in the TCGA database and was significantly correlated with the T stage (P<0.05) and TNM stage (P<0.05) in the GSE13937 database. Functional experiments showed that miR-145-5p attenuated proliferation (P<0.05), migration (P<0.01) and invasion (P<0.01) in the Eca109 cell line. Both GSEA gene enrichment and REACTOME gene enrichment revealed that miR-145-5p was associated with tumor signaling pathways and immune signaling pathways. Immune infiltration analysis revealed that the expression level of miR-145-5p was significantly correlated with the infiltration level of macrophages (P<0.05) and was positively correlated with the level of gene markers of M2 macrophages and tumor-associated macrophages (P<0.05). Conclusions MiR-145-5p acts as a tumor suppressor microRNA in ESCC and is an important noncoding RNA in the high M2-like tumor-associated macrophage infiltration of ESCC. Assessing the miR-145-5p level in ESCC samples has translational meaning, which help illustrate the immune infiltration status, predict the prognostic outcome, and select the type of immunochemotherapy.

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“…DANCR promotes HSP27 expression and facilitates the proliferation and metastasis of CRC cells by inhibiting miR-577 [61]. Various microRNAs, including miR-518a-3p [63], miR-145-5p [65] and miR-185-5p [12], were reported to interact competitively with DANCR, contributing to the aggressive progression of CRC.…”

Section: Colorectal Cancermentioning

confidence: 99%

New evidence for a role of DANCR in cancers: a comprehensive review

Yuan,

Xu,

Zhang

et al. 2024

J Transl Med

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Cancer remains a leading cause of mortality and poses a substantial threat to public health. Studies have revealed that Long noncoding RNA DANCR is a cytoplasmic lncRNA whose aberrant expression plays a pivotal role in various cancer types. Within tumour biology, DANCR exerts regulatory control over crucial processes such as proliferation, invasion, metastasis, angiogenesis, inflammatory responses, cellular energy metabolism reprogramming, and apoptosis. By acting as a competitive endogenous RNA for miRNAs and by interacting with proteins and mRNAs at the molecular level, DANCR contributes significantly to cancer progression. Elevated DANCR levels have also been linked to heightened resistance to anticancer drugs. Moreover, the detection of circulating DANCR holds promise as a valuable biomarker for aiding in the clinical differentiation of different cancer types. This article offers a comprehensive review and elucidation of the primary functions and molecular mechanisms through which DANCR influences tumours.

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Assessment of lncRNA DANCR, miR-145-5p and NRAS axis as biomarkers for the diagnosis of colorectal cancer

Cited by 9 publications

References 29 publications

Activation of lncRNA DANCR by H3K27 acetylation regulates proliferation of colorectal cancer cells

Activation of lncRNA DANCR by H3K27 acetylation regulates proliferation of colorectal cancer cells

The role of miR-145-5p in esophageal squamous cell carcinoma tumor-associated macrophages and selection of immunochemotherapy

New evidence for a role of DANCR in cancers: a comprehensive review

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