Assessment of liver ischemia reperfusion injury in mice using hepatic T<sub>2</sub> mapping: Comparison with histopathology

Hueper, Katja; Lang, H.; Hartleben, Björn; Gutberlet, Marcel; Derlin, Thorsten; Getzin, Tobias; Chen, Rongjun; Abou-Rebyeh, H.; Lehner, Frank; Meier, Martin; Haller, Hermann; Wacker, Frank; Rong, Song; Gueler, Faikah

doi:10.1002/jmri.26057

Cited by 12 publications

(9 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Native T1 relaxation times of the liver have, so far, not been studied extensively, and studies are limited to either patients with liver fibrosis or classifications according to the Child–Pugh score [ 1 , 5 , 6 , 7 , 8 ]. Equally, up to now, only very few and mainly preclinical studies evaluating T2 values have been published regarding the liver [ 9 , 10 ], with even fewer clinical approaches [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Correlation of Native Liver Parenchyma T1 and T2 Relaxation Times and Liver Synthetic Function Tests: A Pilot Study

et al. 2021

View full text Add to dashboard Cite

MR relaxometry increasingly contributes to liver imaging. Studies on native relaxation times mainly describe relation to the presence of fibrosis. The hypothesis was that relaxation times are also influenced by other inherent factors, including changes in liver synthesis function. With the approval of the local ethics committee and written informed consent, data from 94 patients referred for liver MR imaging, of which 20 patients had cirrhosis, were included. Additionally to standard sequences, both native T1 and T2 parametric maps and T1 maps in the hepatobiliary phase of gadoxetate disodium were acquired. Associations with laboratory variables were assessed. Altogether, there was a negative correlation between albumin and all acquired relaxation times in cirrhotic patients. In non-cirrhotic patients, only T1 values exhibited a negative correlation with albumin. In all patients, bilirubin correlated significantly with post-contrast T1 relaxation times, whereas native relaxation times correlated only in cirrhotic patients. Evaluating patients with pathological INR values, post-contrast relaxation times were significantly higher, whereas native relaxation times did not correlate. In conclusion, apart from confirming the value of hepatobiliary phase T1 mapping, our results show a correlation of native T1 with serum albumin even in non-cirrhotic liver parenchyma, suggesting a direct influence of liver’s synthesis capacity on T1 relaxation times.

show abstract

Section: Introductionmentioning

confidence: 99%

Correlation of Native Liver Parenchyma T1 and T2 Relaxation Times and Liver Synthetic Function Tests: A Pilot Study

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Previously, T2 mapping was widely used in detecting and evaluating the degree of tissue edema in inflammatory and ischemic processes as well as degeneration of bones and joints (18)(19)(20). In terms of differentiating malignant from benign lesions, studies had examined its potential value on the tumors involved prostate, breast, and liver, and positive results were obtained (7)(8)(9)(10).…”

Section: Discussionmentioning

confidence: 99%

T2 mapping in orbital masses: preliminary study on differential diagnostic ability of T2 relaxation time

Zhou

et al. 2019

Acta Radiol

View full text Add to dashboard Cite

Background T2 mapping has been proven to be useful in tumor characterization. As to orbital masses, its diagnostic value needs to be investigated. Purpose To evaluate the usefulness of T2 mapping in orbital masses and the ability of T2 relaxation time in differentiating malignant from benign orbital masses. Material and Methods Forty-seven patients with solid orbital masses (33 benign and 14 malignant) who underwent T2 mapping examination for preoperative assessment were enrolled in the current study. T2 mapping was acquired using 16 TE values (range 12–192 ms; delta TE 12 ms). Mean T2 relaxation time was calculated based on the whole mass region of interest and compared between the malignant and benign groups using the unpaired t-test. Receiver operating characteristic curve analysis was adopted to calculate its diagnostic value. Results Malignant orbital masses showed significantly lower T2 relaxation time than benign masses (76.4 ± 13.0 ms vs. 119.1 ± 20.4 ms; P < 0.001). If setting a T2 relaxation time of 89.5 ms as the threshold value, optimal differentiating performance could be achieved (area under the curve 0.936; sensitivity 100.0%; specificity 87.9%; accuracy 91.5%; positive predictive value 77.8%; negative predictive value 100%). Conclusion T2 mapping and its derived T2 relaxation time could provide quantitative information and serve as a supplementary imaging marker for differentiating malignant from benign orbital masses.

show abstract

“…Both Magnetic Resonance Imaging (MRI) and Phasor Fluorescence Lifetime Imaging (FLIM) are promising modalities to evaluate liver function and injury. T2 mapping by MRI has previously been shown as a feasible, non-invasive technique in assessing acute liver injury ( 4 ), while FLIM has been demonstrated to assess for liver injury more accurately via the evaluation of steatosis, fibrosis ( 5 ), and glycolytic activity ( 6 ) in experimental models.…”

Section: Introductionmentioning

confidence: 99%

Oral N-acetylcysteine decreases IFN-γ production and ameliorates ischemia-reperfusion injury in steatotic livers

Liggett¹,

Kang²,

Ranjit³

et al. 2022

Front. Immunol.

View full text Add to dashboard Cite

Type 1 Natural Killer T-cells (NKT1 cells) play a critical role in mediating hepatic ischemia-reperfusion injury (IRI). Although hepatic steatosis is a major risk factor for preservation type injury, how NKT cells impact this is understudied. Given NKT1 cell activation by phospholipid ligands recognized presented by CD1d, we hypothesized that NKT1 cells are key modulators of hepatic IRI because of the increased frequency of activating ligands in the setting of hepatic steatosis. We first demonstrate that IRI is exacerbated by a high-fat diet (HFD) in experimental murine models of warm partial ischemia. This is evident in the evaluation of ALT levels and Phasor-Fluorescence Lifetime (Phasor-FLIM) Imaging for glycolytic stress. Polychromatic flow cytometry identified pronounced increases in CD45+CD3+NK1.1+NKT1 cells in HFD fed mice when compared to mice fed a normal diet (ND). This observation is further extended to IRI, measuring ex vivo cytokine expression in the HFD and ND. Much higher interferon-gamma (IFN-γ) expression is noted in the HFD mice after IRI. We further tested our hypothesis by performing a lipidomic analysis of hepatic tissue and compared this to Phasor-FLIM imaging using “long lifetime species”, a byproduct of lipid oxidation. There are higher levels of triacylglycerols and phospholipids in HFD mice. Since N-acetylcysteine (NAC) is able to limit hepatic steatosis, we tested how oral NAC supplementation in HFD mice impacted IRI. Interestingly, oral NAC supplementation in HFD mice results in improved hepatic enhancement using contrast-enhanced magnetic resonance imaging (MRI) compared to HFD control mice and normalization of glycolysis demonstrated by Phasor-FLIM imaging. This correlated with improved biochemical serum levels and a decrease in IFN-γ expression at a tissue level and from CD45+CD3+CD1d+ cells. Lipidomic evaluation of tissue in the HFD+NAC mice demonstrated a drastic decrease in triacylglycerol, suggesting downregulation of the PPAR-γ pathway.

show abstract

Assessment of liver ischemia reperfusion injury in mice using hepatic T₂ mapping: Comparison with histopathology

Abstract: 1 Technical Efficacy: Stage 2 J. Magn. Reson. Imaging 2018;48:1586-1594.

Cited by 12 publications

References 36 publications

Correlation of Native Liver Parenchyma T1 and T2 Relaxation Times and Liver Synthetic Function Tests: A Pilot Study

Correlation of Native Liver Parenchyma T1 and T2 Relaxation Times and Liver Synthetic Function Tests: A Pilot Study

T2 mapping in orbital masses: preliminary study on differential diagnostic ability of T2 relaxation time

Oral N-acetylcysteine decreases IFN-γ production and ameliorates ischemia-reperfusion injury in steatotic livers

Contact Info

Product

Resources

About

Assessment of liver ischemia reperfusion injury in mice using hepatic T2 mapping: Comparison with histopathology

Abstract: 1 Technical Efficacy: Stage 2 J. Magn. Reson. Imaging 2018;48:1586-1594.

Cited by 12 publications

References 36 publications

Correlation of Native Liver Parenchyma T1 and T2 Relaxation Times and Liver Synthetic Function Tests: A Pilot Study

Correlation of Native Liver Parenchyma T1 and T2 Relaxation Times and Liver Synthetic Function Tests: A Pilot Study

T2 mapping in orbital masses: preliminary study on differential diagnostic ability of T2 relaxation time

Oral N-acetylcysteine decreases IFN-γ production and ameliorates ischemia-reperfusion injury in steatotic livers

Contact Info

Product

Resources

About

Assessment of liver ischemia reperfusion injury in mice using hepatic T₂ mapping: Comparison with histopathology