“…The frequency of differentiated and pyknotic cells, condensed chromatin, karyorrhectic, karyolytic and binucleated cells were determined upon analysis of 1000 cells. The frequency of genotoxicity biomarkers (MNi – micronuclei and NBUDs – nuclear buds) were scored in a minimum of 2000 differentiated cells and reported per 1000 differentiated cells [ 38 , 39 ]. Analysis was done according to scoring criteria proposed by Thomas et al (2009) [ 27 ].…”