Assessment of light transmission aggregometry on the routine coagulation analyzer Sysmex CS-2500 using CE-marked agonists from Hyphen Biomed

Bret, V.-E.; Pougault, B.; Guy, Alexandre; Castet, Sabine; Huguenin, Yoann; Pillois, Xavier; James, Chloé; Fiore, Mathieu

doi:10.1080/09537104.2018.1528346

Cited by 7 publications

(8 citation statements)

References 9 publications

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“…With both LTA and release assays, single agonist abnormalities are usually a false positive and are much less predictive of a bleeding disorder than multiple agonist abnormalities . Some laboratories perform LTA on automated coagulation analyzers, which appears to provide comparable findings to traditional LTA . Many laboratories incorrectly interpret LTA findings that show normal variants or false positives (eg, reduced MA with a single agonist), and/or fail to consider all potential causes for multiple agonist abnormalities .…”

Section: Resultsmentioning

confidence: 99%

“…26,27,31 Some laboratories perform LTA on automated coagulation analyzers, which appears to provide comparable findings to traditional LTA. 32 Many laboratories incorrectly interpret LTA findings that show normal variants or false positives (eg, reduced MA with a single agonist), and/or fail to consider all potential causes for multiple agonist abnormalities. 33 Such errors could be prevented by following guideline recommendations on LTA interpretation.…”

Section: Interpretation Of Platelet Function Tests Performed With Mmentioning

confidence: 99%

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Update on diagnostic testing for platelet function disorders: What is practical and useful?

Hayward

Moffat

Brunet

et al. 2019

Int J Lab Hematology

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Introduction Platelet function disorders (PFD) are an important group of bleeding disorders that require validated and practical laboratory strategies for diagnosis. Methods This review summarizes the authors’ experiences, current literature, and an international survey to evaluate the practices of diagnostic laboratories that offer tests for PFD. Results Blood counts, blood film review, and aggregation tests are the most commonly performed investigations for PFD and help determine whether there is thrombocytopenia and/or defective platelet function due to a variety of causes. The performance characteristics of tests for PFD, and the level of evidence that these tests detect bleeding problems, are important issues to determine where tests are useful for diagnostic or correlative purposes, or research only uses. Platelet aggregation assays, and quantitative analysis of platelet dense granule numbers, are tests with good performance characteristics that detect abnormalities associated with increased bleeding in a significant proportion of individuals referred for PFD investigations. Lumiaggregometry estimates of platelet adenosine triphosphate release show greater variability which limits the diagnostic usefulness. Diagnostic laboratories report that fiscal and other constraints, including a lack of high‐quality evidence, limit their ability to offer an expanded test menu for PFD. Conclusion PFD are clinically important bleeding disorders that remain challenging for diagnostic laboratories to investigate. While some PFD tests are well validated for diagnostic purposes, gaps in scientific evidence and resource limitations influence diagnostic laboratory decisions on which PFD tests to offer.

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Section: Resultsmentioning

confidence: 99%

Section: Interpretation Of Platelet Function Tests Performed With Mmentioning

confidence: 99%

Update on diagnostic testing for platelet function disorders: What is practical and useful?

Hayward

Moffat

Brunet

et al. 2019

Int J Lab Hematology

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“…Furthermore, promising results are accomplished with platelet aggregation studies under flow conditions [28]. Advantages of these new methods are the use of whole blood and low sample preparation, but they also require trained technicians and specialized equipment [29][30][31][32][33][34]. Likewise, standardization of the traditional LTA method is still essential here.…”

Section: Discussionmentioning

confidence: 99%

Harmonizing light transmission aggregometry in the Netherlands by implementation of the SSC-ISTH guideline

et al. 2020

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Light transmission aggregometry (LTA) is considered the gold standard method for evaluation of platelet function. However, there are a lot of variation in protocols (pre-analytical procedures and agonist concentrations) and results. The aim of our study was to establish a national LTA protocol, to investigate the effect of standardization and to define national reference values for LTA. The SSC guideline was used as base for a national procedure. Almost all recommendations of the SSC were followed e.g. no adjustment of PRP, citrate concentration of 109 mM, 21 needle gauge, fasting, resting time for whole blood and PRP, centrifugation time, speed and agonists concentrations. LTA of healthy volunteers was measured in a total of 16 hospitals with 5 hospitals before and after standardization. Results of more than 120 healthy volunteers (maximum aggregation %) were collected, with participating laboratories using 4 different analyzers with different reagents. Use of low agonist concentrations showed high variation before and after standardization, with the exception of collagen. For most high agonist concentrations (ADP, collagen, ristocetin, epinephrine and arachidonic acid) variability in healthy subjects decreased after standardization. We can conclude that a standardized Dutch protocol for LTA, based on the SSC guideline, does not result in smaller variability in healthy volunteers for all agonist concentrations.

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“…The instrument then carries out automated light transmission aggregometry. In instances where it has been compared head-to-head with traditional light transmission aggregometry ( Table 2 ), the Sysmex CS-2000i has shown repeatability, reproducibility and agreement with traditional aggregometers [ 15 , 16 , 17 , 18 ]. While the technology is certainly advantageous in terms of labour requirement, it still calls for an appreciable volume of platelet-rich plasma (140 µL per test) albeit less than traditional aggregometry (200–500 µL per test) [ 16 ].…”

Section: Light Transmission Aggregometry Revisitedmentioning

confidence: 99%

Section: Light Transmission Aggregometry Revisitedmentioning

confidence: 99%

Advances in Platelet Function Testing—Light Transmission Aggregometry and Beyond

Blanc

Mullier²,

Vayne

et al. 2020

JCM

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Platelet function testing is essential for the diagnosis of hemostasis disorders. While there are many methods used to test platelet function for research purposes, standardization is often lacking, limiting their use in clinical practice. Light transmission aggregometry has been the gold standard for over 60 years, with inherent challenges of working with live dynamic cells in specialized laboratories with independent protocols. In recent years, standardization efforts have brought forward fully automated systems that could lead to more widespread use. Additionally, new technical approaches appear promising for the future of specialized hematology laboratories. This review presents developments in platelet function testing for clinical applications.

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Assessment of light transmission aggregometry on the routine coagulation analyzer Sysmex CS-2500 using CE-marked agonists from Hyphen Biomed

Cited by 7 publications

References 9 publications

Update on diagnostic testing for platelet function disorders: What is practical and useful?

Update on diagnostic testing for platelet function disorders: What is practical and useful?

Harmonizing light transmission aggregometry in the Netherlands by implementation of the SSC-ISTH guideline

Advances in Platelet Function Testing—Light Transmission Aggregometry and Beyond

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