Assessment of laser Doppler perfusion imager’s <i>in vivo</i> reliability: Can it be used for a prospective analysis?

Caselli, Antonella; Bedi, Damanpreet S.; O’Connor, Colleen; Shah, Chirag; Veves, Aristidis

doi:10.2351/1.1494084

Cited by 5 publications

(3 citation statements)

References 16 publications

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“…Blood perfusion is commonly assessed by non-invasive Laser Doppler Imaging (LDI), which measures blood flow through backscattering a laser beam that interacts with moving red blood cells [12,13]. While the non-invasive nature of LDI is highly desirable, it suffers from a lack of standardisation, poor temporal resolution, and can only penetrate tissue to a maximum depth of 10 mm [10].…”

Section: Introductionmentioning

confidence: 99%

In vitro development and in vivo application of a platinum-based electrochemical device for continuous measurements of peripheral tissue oxygen

Finnerty

Bolger

2018

Bioelectrochemistry

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Acute limb ischaemia is caused by compromised tissue perfusion and requires immediate attention to reduce the occurrence of secondary complications that could lead to amputation or death. To address this, we have developed a novel platinum (Pt)-based electrochemical oxygen (O) device for future applications in clinical monitoring of peripheral tissue ischaemia. The effect of integrating a Pt pseudo-reference electrode into the O device was investigated in vitro with an optimum reduction potential of -0.80V. A non-significant (p=0.11) decrease in sensitivity was recorded when compared against an established Pt-based O sensor operating at -0.65V. Furthermore, a biocompatible clinical sensor (ClinOX) was designed, demonstrating excellent linearity (R=0.99) and sensitivity (1.41±0.02nAμM) for O detection. Significant rapid decreases in the O current during in vivo ischaemic insults in rodent limbs were reported for Pt-Pt (p<0.001) and ClinOX (p<0.01) and for ClinOX (p<0.001) in porcine limbs. Ex vivo sensocompatibility investigations identified no significant difference (p=0.08) in sensitivity values over 14days of exposure to tissue homogenate. The Pt-Pt based O design demonstrated high sensitivity for tissue ischaemia detection and thus warrants future clinical investigation.

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Section: Introductionmentioning

confidence: 99%

In vitro development and in vivo application of a platinum-based electrochemical device for continuous measurements of peripheral tissue oxygen

Finnerty

Bolger

2018

Bioelectrochemistry

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“…In a recent study, Caselli et al (2002) concluded that LDI is a reliable and reproducible technique to assess the skin blood flow with a coefficient of variation of 16%. The error for the vessel diameter measurement by the automated detection algorithm is 0.5 pixel (Sun et al, 1995), or 8 m for the resolution of the digitized micrographs in this study.…”

Section: Discussionmentioning

confidence: 99%

Volumetric flow mapping for microvascular networks by bimodality imaging with light microscope and laser doppler imager

Sun

Verbeuren

Vallez

et al. 2004

Microscopy Res & Technique

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A method was developed to produce a composite image of microvascular networks with grayscales proportional to volumetric flows. Velocities in arterioles and venules were assessed with a high-resolution laser Doppler imager (LDI). The vascular structures were quantified from the micrograph with a computerized vessel detection algorithm. After registering the detected vascular network with the LDI scan, volumetric flows were calculated along the centerlines of the vessels. In vivo data were obtained from the hamster cheek pouch in 6 studies. Flow continuity of the flow map was evaluated by comparing the main flow (Q) with the sum of branch flows (Qs), averaging over the respective vessel segments incident to each bifurcation. The method was reproducible across the 6 studies with the correlation coefficient (r) between Qs and Q ranging from 0.913 to 0.986. In all, over 20,000 flow estimates from 360 vessel segments (24-160 microm in diameter) at 166 bifurcations were analyzed. With flow normalized between 0 and 1, the linear regression yielded: Qs = 1.03 Q + 0.006; r = 0.952, n = 166, P < 0.0005. The bimodality imaging method exploits a large amount of velocity and diameter data, and therefore should be useful for studying heterogeneous flows in the microvasculature.

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“…This technique is capable of noninvasive and continuous recording of blood perfusion in tissue. A major limitation of laser Doppler technique is its inability to image tissue deeper than 1 mm [14][15][16][17][18]. Infrared thermography graphically depicts temperature gradients over a given body surface area.…”

Section: Introductionmentioning

confidence: 99%

Measurement of blood perfusion using photoacoustic, ultrasound, and strain imaging

Mallidi

Karpiouk

Aglyamov

et al. 2007

Photons Plus Ultrasound: Imaging and Sensing 2007: The Eighth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acou

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In many clinical and research applications including cancer diagnosis, tumor response to therapy, reconstructive surgery, monitoring of transplanted tissues and organs, and quantitative evaluation of angiogenesis, sequential and quantitative assessment of microcirculation in tissue is required. In this paper we present an imaging technique capable of spatial and temporal measurements of blood perfusion through microcirculation. To demonstrate the developed imaging technique, studies were conducted using phantoms with modeled small blood vessels of various diameters positioned at different depths. A change in the magnitude of the photoacoustic signal was observed during vessel constriction and subsequent displacement of optically absorbing liquid present in the vessels. The results of the study suggest that photoacoustic, ultrasound and strain imaging could be used to sequentially monitor and qualitatively assess blood perfusion through microcirculation.

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Assessment of laser Doppler perfusion imager’s in vivo reliability: Can it be used for a prospective analysis?

Cited by 5 publications

References 16 publications

In vitro development and in vivo application of a platinum-based electrochemical device for continuous measurements of peripheral tissue oxygen

In vitro development and in vivo application of a platinum-based electrochemical device for continuous measurements of peripheral tissue oxygen

Volumetric flow mapping for microvascular networks by bimodality imaging with light microscope and laser doppler imager

Measurement of blood perfusion using photoacoustic, ultrasound, and strain imaging

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