Assessment of Lactic Acid Bacteria and Enterobacteriaceae Counts in Bulgarian Probiotic Products by TEMPO® System and ISO Methods

Abstract

Submit Manuscript | http://medcraveonline.com pathogenic bacteria. Therefore, rigorous hygienic precautions and adequate monitoring of the process environment and the finished product should be in place in order to ensure the quality and safety of the final product.The common microbiological parameters monitored for quality and safety of dairy products are applicable to probiotic products as well. Of these, Enterobacteriaceae is usually tested as a hygiene indicator intended to verify the hygiene programs applied at the production premises [2,3].Microbiological testing for the presence of Enterobacteriaceae is largely applied for dairy products, and some pathogenic species of this family, such as E. sakazakii, represent a significant risk related to powdered milk products. A FAO meeting report [4] states that the presence of Enterobacteriaceae in packaged powdered infant formulas is not due to the organisms surviving the process, but rather as a result of recontamination which is due to post-heating processes such as drying, conveying, tipping, mixing with additional ingredients and filling or packaging. Recontamination could also be related to the presence of microorganisms in the processing environment and to equipment that is in contact with the product [5].Dry dairy-based probiotic formulations are of the same nature as powdered milk products in terms of food matrices and production process, and therefore testing for Enterobacteriaceae as a hygienic indicator should be applied for these products as well, with certain exceptions, when a representative of Enterobacteriaceae (e.g. E. coli Nissle 1917) is an intended part of the probiotic formulation.

Antimicrobial Activity and Prevention of Bacterial Biofilm Formation of Silver and Zinc Oxide Nanoparticle-Containing Polyester Surfaces at Various Concentrations for Use

“…The TEMPO system is an automated most probable number (MPN), which is based on the measurements of fluorescence method for enumerating aerobic bacteria and specific pathogens in foods [48][49][50]. Further, our results are in accordance with those observed by Katase and Tsumura [51], Blagoeva et al [52], and Łobacz et al [53], who compared the results of the TEMPO system with the corresponding methods to enumerate total aerobic bacteria, coliforms, Enterobacteriaceae, yeast and mold, S. aureus, and lactic acid bacteria. Moreover, comparisons of different methods for quantifying L. monocytogenes biofilms have shown that among the indirect counting methodologies, the one that gives the best results is the TEMPO system [54].…”

Assessing Viability and Stress Tolerance of Probiotics—A Review

“…The most probable number method is based on that a liquid medium turns turbid during bacterial growth (Bibiloni et al, 2001;Sutton, 2010;Angelov, 2014). By making a 10-fold serial dilution of a bacterial cell solution in replicates, the point will be reached where the solution is so diluted that only a few of the replicates of a certain dilution give rise to turbidity.…”

“…The number of tubes with confirmed turbidity, thus growth, at the different dilutions can be used to estimate the number of viable cells in the original sample by utilizing premade tables based on statistics using 95% confidence intervals. Although not traditionally used for viability measurements in probiotics, there are a few examples (Bibiloni et al, 2001;Angelov, 2014).…”