Assessment of Kidney Function Indices in Male Albino Wistar Rats Administered Ethanol Stem Extract of Dennettia tripetala (Pepper fruit)

Nwankpa, Promise; Cn, Ekweogu; Egwurugwu, JN; Og, Chukwuemeka; Cc, Etteh; Pc, Ugwuezumba; Fc, Emengagha

doi:10.4172/2167-0501.1000242

Cited by 4 publications

(3 citation statements)

References 13 publications

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“…The renal toxicity induced by Pausinystalia yohimbe bark extract is indicative of some degree of toxicity associated with the use of the extract and could be a result of high amounts in the extract, of bioactive substances including yohimbine [14], alkaloids, flavonoids, terpenoids, resins, tannins and saponins [42], some of which have been implicated in toxicity when taken beyond tolerable limits. This result supports the result of Nwankpa et al [34] on ethanol stem extract of Dennettia tripetala in Wistar rats. Their report suggested that the presence of tissue-damaging alkaloids and saponins may have contributed to renal toxicity.…”

Section: Discussionsupporting

confidence: 92%

“…Kuwabara et al [32] in their study identified elevated serum sodium as an independent risk factor for the development of chronic kidney disease. The findings of Nwankpa et al [34] and Oyetunji et al [35] on the effects of Dennettia tripetala stem extract and Newbouldia laevis leaf on Wistar rats are supported by this result. Their report suggested that the extracts may have contributed to renal damage via hypernatraemic effect.…”

Section: Discussionsupporting

confidence: 66%

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Effects of Graded Doses of Ethanolic Extract of Pausinystalia Yohimbe Stem Bark on Biochemical and Hematological Indices In the Kidneys of Female Wistar Rats

Okwakpam

Ken-Ezihuo

P.C.

et al. 2023

IJBcRR

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Pausinystalia yohimbe is a medicinal plant widely used as an aphrodisiac agent in Nigeria and other parts of West Africa, but little is known about its effect on renal function after chronic use. This study was designed to investigate the effect of ethanolic extract of Pausinystalia yohimbe stem bark on renal function parameters such as serum creatinine, urea, electrolyte, oxidative stress markers, and hematological parameters in female Wistar rats. Ethanolic extract of Pausinystalia yohimbe stem bark was administered orally to 3 groups of 5 female rats for 21 days as follows: group1 which served as control received distilled water while groups 2 and 3 received 400mg/kg and 800mg/kg body weight of the extract respectively. Thereafter the rats were sacrificed, and blood and kidney tissues were collected for biochemical analysis. The result showed that there were no significant changes in urea, creatinine, potassium, chloride, and bicarbonate levels while sodium level was significantly increased (p<0.05) when compared to the control. SOD and CAT tissue activities did not show any significant change (p<0.05) while there was a significant increase (p<0.05) in GSH and a significant reduction (p<0.05) in MDA. Also, there was no significant change (p<0.05) in WBC, PCV, and platelets, however, there was a significant decrease (p<0.05) in RBC and hemoglobin levels. The histopathology of the kidney showed that no significant changes were observed between treatment groups and control except for the 800 mg/kg body weight of the extract-treated group where there was mild multifocal vacuolar degeneration of some renal tubular epithelial cells. The result suggests that the chronic use of the ethanolic extract of Pausinystalia yohimbe stem bark protects the kidney against oxidative stress but adversely affects the functional capacities of the kidney and formation of erythropoietin in the stem cells and may also contribute to the increasing number of persons with high blood pressure.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 66%

Effects of Graded Doses of Ethanolic Extract of Pausinystalia Yohimbe Stem Bark on Biochemical and Hematological Indices In the Kidneys of Female Wistar Rats

Okwakpam

Ken-Ezihuo

P.C.

et al. 2023

IJBcRR

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“…aminotransferase (ALT) [38]. Other procedures adopted were as described for creatinine [39], urea [39], and uric acid [40]. Serum insulin concentration determination was achieved based on the method described by [32], which used an ELISA kit from Sweden in a multiple plate ELISA reader (Winooski, Vermont, USA).…”

Section: Plos Onementioning

confidence: 99%

Spilanthes filicaulis (Schumach. & Thonn.) C.D. Adams leaves protects against streptozotocin-induced diabetic nephropathy

Ojo,

Ogunlakin,

Akintayo

et al. 2024

PLoS ONE

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Background and objective Diabetic neuropathy (DN) is a complex type of diabetes. The underlying cause of diabetic nephropathy remains unclear and may be due to a variety of pathological conditions resulting in kidney failure. This study examines the protective effect of the methanolic extract of Spilanthes filicaulis leaves (MESFL) in fructose-fed streptozotocin (STZ)-induced diabetic nephropathy and the associated pathway. Methods Twenty-five rats were equally divided randomly into five categories: Control (C), diabetic control, diabetic + metformin (100 mg/kg), diabetic + MESFL 150 mg/kg bw, and diabetic + MESFL 300 mg/kg bw. After 15 days, the rats were evaluated for fasting blood glucose (FBG), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), urea, uric acid, serum creatinine, reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), and lipid peroxidation (MDA). Gene expression levels of cyclic adenosine monophosphate (cAMP), protein kinase A (PKA), cAMP response element-binding (CREB), cFOS and the antiapoptotic protein Bcl-2 were examined. Results We observed that MESFL at 150 and 300 mg/kg bw significantly downregulated the protein expression of cAMP, PKA, CREB, and cFOS and upregulated the Bcl-2 gene, suggesting that the nephroprotective action of MESFL is due to the suppression of the cAMP/PKA/CREB/cFOS signaling pathway. In addition, MESFL increases SOD and CAT activities and GSH levels, reduces MDA levels, and reduces renal functional indices (ALP, urea, uric acid, and creatinine). Conclusion Therefore, our results indicate that MESFL alleviates the development of diabetic nephropathy via suppression of the cAMP/PKA/CREB/cFOS pathways.

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Preclinical antidiabetic and antioxidant effects of Erythrophleum africanum (benth.) harms in streptozotocin-induced diabetic nephropathy

Ojo,

Ajeigbe,

Ogunlakin

et al. 2024

Journal of Complementary and Integrative Medicine

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Objectives This study investigated the antidiabetic effects of the methanolic extract of E. africanum (MEEA) stem bark on streptozotocin (STZ)-induced diabetic nephropathy (DN) in Wistar rats. Methods The in vitro enzyme (α-amylase) inhibitory activity of MEEA was measured using a standard procedure. Diabetic rats with fasting blood glucose above 250 mg/dL were considered diabetic and were divided into the following groups: control (distilled water-treated), diabetic-control, diabetic metformin (100 mg/kg), diabetes + MEEA (150 mg/kg), and diabetes + MEEA (300 mg/kg) via oral gavage once daily for 14 days. At the end of the experimental period, kidney tissues were collected for biochemical and histological analyses. Kidney apoptosis and marker gene expression were measured by real-time quantitative PCR. Results MEEA exhibited α-amylase inhibitory effects. MEEA significantly (p<0.05) reduced the STZ-induced increases in blood glucose, serum urea, serum creatinine, uric acid, alanine aminotransferase, alkaline phosphatase, and malondialdehyde and increased the STZ-induced decreases in superoxide dismutase, catalase, and reduced glutathione. In addition, MEEA protects against DN by significantly downregulating the mRNA expression of cyclic adenosine monophosphate (cAMP), protein kinase A (PKA), cAMP-response binding protein (CREB), and cFOS and upregulating B-cell lymphoma 2 (Bcl-2), suggesting that the nephroprotective ability of MEEA is due to the modulation of the cAMP/PKA/CREB/cFOS signaling pathway. Furthermore, MEEA treatment protected against histopathological alterations observed in diabetic rats. Conclusions The data from this study suggest that MEEA modulates glucose homeostasis and inhibits redox imbalance in DN rats.

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Assessment of Kidney Function Indices in Male Albino Wistar Rats Administered Ethanol Stem Extract of Dennettia tripetala (Pepper fruit)

Cited by 4 publications

References 13 publications

Effects of Graded Doses of Ethanolic Extract of Pausinystalia Yohimbe Stem Bark on Biochemical and Hematological Indices In the Kidneys of Female Wistar Rats

Effects of Graded Doses of Ethanolic Extract of Pausinystalia Yohimbe Stem Bark on Biochemical and Hematological Indices In the Kidneys of Female Wistar Rats

Spilanthes filicaulis (Schumach. & Thonn.) C.D. Adams leaves protects against streptozotocin-induced diabetic nephropathy

Preclinical antidiabetic and antioxidant effects of Erythrophleum africanum (benth.) harms in streptozotocin-induced diabetic nephropathy

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