Assessment of Kidney Function in Mouse Models of Glomerular Disease

Stevens, M.F.; Oltean, Sebastian

doi:10.3791/57764-v

Cited by 2 publications

(1 citation statement)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The serum was stored in a refrigerator (4–8°C) for blood urea, creatinine, sodium, potassium, and chloride analysis. 54 , 55 …”

Section: Methodsmentioning

confidence: 99%

Nephroprotective Effect of the Leaf Extract of Ajuga remota Benth Against Gentamicin-Induced Nephrotoxicity in Swiss Albino Mice

Akinaw,

P Nair,

Usure

et al. 2024

JEP

View full text Add to dashboard Cite

Background Drug-induced kidney injury was among the most common renal damages, from which gentamicin occupies around 25% of this injury. Gentamicin-induced renal damage is caused by increased free radicals with subsequent amplified inflammation. Ajuga remota leaf extract has many phytochemicals with antioxidant activities, which may improve gentamicin-induced renal damage. Thus, we aimed to investigate the nephroprotective effect of Ajuga remota leaf methanolic extract on gentamicin-induced nephrotoxicity in Swiss Albino Mice. Methods An experimental study design was used on 30 experimental mice randomly allocated in six groups: Group I, II, II, IV, and VI, among which mice were given only distilled water, only gentamicin, 600 mg/kg Ajuga remota leaf extract only, gentamicin along with 200 mg/kg extract, gentamicin with 400 mg/kg extract and gentamicin with 600 mg/kg extract, respectively. At the end of the experiment, the mice were sacrificed after being anaesthetized, and blood samples were collected through a cardiac puncture for renal function tests while the kidneys were removed for histopathological evaluation. The data were entered into Epidata version 4.6 and exported to SPSS version 25 for further analysis using one-way analysis of variance. Statistical significance was set at p < 0.05. Results Group II mice had significantly higher levels of serum creatinine and blood urea levels compared to group I and III. The body weight of the mice in group V and group VI showed a significant increase compared with Group II. Serum creatinine and blood urea levels were reduced significantly in the Ajuga remota leaf extract administered group of mice compared to group II. Abnormal kidney architectural changes were seen among group II mice; however, those changes were improved after administration of Ajuga remota leaf methanolic extract. Conclusion Methanol extract of Ajuga remota leaf provided effective protection against gentamicin-induced oxidative renal damage through its antioxidant effects.

show abstract

“…The serum was stored in a refrigerator (4–8°C) for blood urea, creatinine, sodium, potassium, and chloride analysis. 54 , 55 …”

Section: Methodsmentioning

confidence: 99%

Nephroprotective Effect of the Leaf Extract of Ajuga remota Benth Against Gentamicin-Induced Nephrotoxicity in Swiss Albino Mice

Akinaw,

P Nair,

Usure

et al. 2024

JEP

View full text Add to dashboard Cite

show abstract

Animal Models of Diabetic Nephropathy

Azizah

Bahtiar

Angelina

2023

Asian J Pharm Clin Res

View full text Add to dashboard Cite

Diabetic nephropathy (DN) is the most common complication of diabetes mellitus (DM). It is characterized by high blood glucose levels or hyperglycemia and is accompanied by changes in lipid, carbohydrate, and protein metabolism which can lead to an increased risk of complications due to vascular disease. DN is probably the most insidious among these complications, causing substantial morbidity and mortality. In this article, we will review the literature on animal models of diabetes. We will discuss several species as animal models for Type 1 and 2 diabetes, including zebrafish, rabbits, mice, rats, and rat models. This article also provides various methods used in research with model animals and presents the required result for studying diabetic DN.

show abstract

Assessment of Kidney Function in Mouse Models of Glomerular Disease

Cited by 2 publications

References 1 publication

Nephroprotective Effect of the Leaf Extract of Ajuga remota Benth Against Gentamicin-Induced Nephrotoxicity in Swiss Albino Mice

Nephroprotective Effect of the Leaf Extract of Ajuga remota Benth Against Gentamicin-Induced Nephrotoxicity in Swiss Albino Mice

Animal Models of Diabetic Nephropathy

Contact Info

Product

Resources

About