Assessment of Key Factors Impacting Variability in AAV Vector Genome Titration by Digital PCR
Guangyu Wang,
Qiang Ma,
Changlong Wei
et al.
Abstract:Recombinant adeno-associated virus (rAAV) has emerged as a prominent vector for in vivo gene therapy, owing to its distinct advantages. Accurate determination of the rAAV genome titer is crucial for ensuring the safe and effective administration of clinical doses. The evolution of the rAAV genome titer assay from quantitative PCR (qPCR) to digital PCR (dPCR) has enhanced accuracy and precision, yet practical challenges persist. This study systematically investigated the impact of various operational factors on… Show more
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