Background: The current rapid prevalence of the novel coronavirus (COVID-19) pandemic over the world is associated with a considerable level of scared, anxiety and panic among the public, in particular, healthcare providers (HCPs) as the most vulnerable group at risk for SARS-CoV-2 infection. To protect and enhance HCPs' capacity to carry out their duties in responding to COVID-19, healthcare authorities should keep them away from stress and anxiety and improve their knowledge, attitude, and practice towards COVID-19 to ensure their compliance to infection control precautions. Therefore, this study aimed to explore knowledge, attitudes, anxiety, and preventive behaviours among Yemeni HCPs towards COVID-19.Methods: A descriptive, web-based-cross-sectional study was used. It was conducted among a number of 1244 Yemeni HCPs. The COVID-19 related questionnaire was designed using Google forms. The link of the survey was sent to the respondents via emails, WhatsApp messages and WhatsApp groups as well as Telegram and other social media. The data collection started on 22nd April 2020, at 6 PM and closed on 26th April 2020 at 11 AM.Results: The results indicated that out of 1244 of the Yemeni HCPs participating in this study, 61.3% were male and more than (67%) were in the age group of 20 to 30 years old with a mean of 29.25±6.75. Most of them (85.9%) had a bachelor degree or above and had 10 years of working experience or less (88.2%). Besides, although 57.1% of the respondents obtained their information via social and news media and 60.3% had never attended lectures/discussions about COVID-19, the results revealed that the majority of respondents had an adequate level of knowledge, an optimistic attitude, moderate anxiety and high performance in preventive behaviors (60.3%, 85.00%, 50.80 & 87.60%, respectively) toward the COVID-19 pandemic.Conclusion: Although the Yemeni HCPs exhibited an adequate level of knowledge, an optimistic attitude, moderate anxiety and high performance in preventive behaviours toward the COVID-19 pandemic, the results showed gaps in some themes, particularly in their knowledge and attitude on COVID-19 pandemic which need to be updated or refreshed.